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EMAGE:11362

Btbd3 BTB (POZ) domain containing 3 ( MGI:2385155)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11362 EMAGE:11362 EMAGE:11362 EMAGE:11362 EMAGE:11362
"Pseudo-wholemount" of euxassay_006662. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006662_01 euxassay_006662_02 euxassay_006662_03 euxassay_006662_04
EMAGE:11362 EMAGE:11362 EMAGE:11362 EMAGE:11362 EMAGE:11362
euxassay_006662_05 euxassay_006662_06 euxassay_006662_07 euxassay_006662_08 euxassay_006662_09
EMAGE:11362 EMAGE:11362 EMAGE:11362 EMAGE:11362 EMAGE:11362
euxassay_006662_10 euxassay_006662_11 euxassay_006662_12 euxassay_006662_13 euxassay_006662_14
EMAGE:11362 EMAGE:11362 EMAGE:11362 EMAGE:11362 EMAGE:11362
euxassay_006662_15 euxassay_006662_16 euxassay_006662_17 euxassay_006662_18 euxassay_006662_19
EMAGE:11362 EMAGE:11362 EMAGE:11362 EMAGE:11362 EMAGE:11362
euxassay_006662_20 euxassay_006662_21 euxassay_006662_22 euxassay_006662_23 euxassay_006662_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11362Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11362_wholemount_strong.wlz
11362_wholemount_moderate.wlz
11362_wholemount_weak.wlz
11362_wholemount_possible.wlz
11362_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11362_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
diaphragm
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 11 16 17 18 19 20 21 22 23 24
forearm mesenchyme
strong strong
regionalstrong expression: see section 01 02
upper arm mesenchyme
strong strong
regionalstrong expression: see section 02 03
forelimb digit 1 phalanx
strong strong
regionalstrong expression: see section 04 05 06
forelimb digit 2 phalanx
strong strong
regionalstrong expression: see section 04 05 06
forelimb digit 3 phalanx
strong strong
regionalstrong expression: see section 04
hand mesenchyme
strong strong
regionalstrong expression: see section 03
hindlimb digit 1 phalanx
strong strong
regionalstrong expression: see section 05
hindlimb digit 2 phalanx
strong strong
regionalstrong expression: see section 05 06 07 08 09
hindlimb digit 3 phalanx
strong strong
regionalstrong expression: see section 05 06 07 08 09
hindlimb digit 4 phalanx
strong strong
regionalstrong expression: see section 05 06 07 08 09
hindlimb digit 5 phalanx
strong strong
regionalstrong expression: see section 07 08
knee mesenchyme
strong strong
regionalstrong expression: see section 01 02
lower leg mesenchyme
strong strong
regionalstrong expression: see section 03 04
upper leg mesenchyme
strong strong
regionalstrong expression: see section 02
pectoralis major
strong strong
regionalstrong expression: see section 11 12 13 14 15 16 17 18 19 20 21 22
pectoralis minor
strong strong
regionalstrong expression: see section 11 12 13 14 15 16 17 18 19 20 21 22
adenohypophysis
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16
hypothalamus mantle layer
strong strong
regionalstrong expression: see section 16 18 moderate expression: see section 07 08 09 10 11 12 13 15
diencephalon lateral wall mantle layer
strong strong
regionalstrong expression: see section 16 18 moderate expression: see section 07 08 09 10 11 12 13 15 19
telencephalon mantle layer
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 14 15 16 23 24 moderate expression: see section 11 12 13 17 18 19 20 21 22
telencephalon marginal layer
strong strong
regionalstrong expression: see section 01
olfactory cortex marginal layer
moderate moderate
regionalmoderate expression: see section 12 20
medulla oblongata basal plate mantle layer
strong strong
regionalstrong expression: see section 08 09 10 11 13 14 15 16 17
rest of cerebellum marginal layer
strong strong
regionalstrong expression: see section 03 04 06 07 08 09 10 11 13 14 15 16 17 18 20 moderate expression: see section 05 21
pons mantle layer
strong strong
regionalstrong expression: see section 06 07 17 18
midbrain mantle layer
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 15 16 17 18 19 moderate expression: see section 05
midbrain marginal layer
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 06 07 18 weak expression: see section 19
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 09 18 weak expression: see section 08 19 20 21 22 23
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 09 17 18 weak expression: see section 08
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 07 09 weak expression: see section 08 19 20
ventral grey horn
strong strong
regionalstrong expression: see section 15 16 17 moderate expression: see section 08 09 10 11 12 13 14
cervico-thoracic ganglion
moderate moderate
regionalmoderate expression: see section 09 10 weak expression: see section 17
cervical ganglion
moderate moderate
regionalmoderate expression: see section 09 weak expression: see section 17
thoracic ganglion
weak weak
regionalweak expression: see section 14
anterior naris
moderate moderate
regionalmoderate expression: see section 17
posterior naris
moderate moderate
regionalmoderate expression: see section 17 18
tongue epithelium
strong strong
regionalstrong expression: see section 11 12 13 14 15 16 17 18 19 20
lower lip
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 19 20 21 22
not examined not examined
regionalnot examined expression: see section 11
upper lip
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
trachea cartilaginous ring
strong strong
regionalstrong expression: see section 13 14
axial skeleton
moderate moderate
regionalmoderate expression: see section 12 13 14 15 16
clavicle
strong strong
regionalstrong expression: see section 10 17
axial skeleton tail region
moderate moderate
regionalmoderate expression: see section 15 16 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7062
Entity Detected:Btbd3, BTB (POZ) domain containing 3 ( MGI:2385155)
Sequence:sense strand is shown

>T7062
CACAACTTCACCGGCCGGCCGTGGCCGCACTGACTCGCTGGGCTCGCCACATCTTGCTAACCTAATTCAG
AAAGCAAGCGCCTCAGCTCTCTCTCTGCCTGCCTCCTTTGCTCAGCTTTGCAGGTGATCCAGAGAACGGC
TGGTTTTATGCGCAAGTAAGAAAACCAGGAATCATGATGGGAATGTATTTAACAGATCCGGTCCTGGATA
AAACTTAAAGCCAGTTTCTCTTCAACATAGTGAAAAGGTCACCTTGCCTGGCTGGGAAAAGCAGGAAGAA
AGCAGCCTGCCGGTTTCTGTTAACATCTTAGCTCCAGCTAGCCTCTTTGCCTTGATGTTTGAGCAGTTTT
GCGAGATCTAGCTCTAAAGAGAGACATCCTGTACTCATGGTAGATGATAAAGAAAAGAACATGAAATGTC
TCACCTTCTTCTTGATGCTTCCAGAGACAGTAAAGAACAGGTCCAAAAAAGGTTCAAAGAAAGCCAACAG
CAGCGGTGGCGGCGGCGGCGGCGGCAGCGTTGGCAGTGGGAGCAGCAAGTTGCCCCCAGTTTGTTATGAA
ATAATTACCTTGAAGACTAAAAAGAAGAAGAAGATGGCTGCTGATATATTCCCCCGTAAGAAGCCAGCCA
ACTCCAGCAGCACCACTGTCCAGCAGCAGCACCAGCACCAGCACAACCTCTGTAACAACAACCTCATCCC
GGCCCCCAACTGGCAGGGTCTTTATCCCACCATCCGAGAAAGAAATGCGGTGATGTTCAATAATGACTTG
ATGGCAGACGTACATTTTGTGGTTGGGCCACCAGGTGGGACTCAGCGGTTGCCAGGACACAAATACGTCC
TCGCTGTCGGGAGCTCAGTGTTCCATGCAATGTTTTACGGAGAACTCGCTGAGGACAAAGATGAAATCCG
AATCCCAGACGTCGAACCTGCTGCCTTCCTCGCCATGCTGAAATACATCTATTGTGATGAAATTGACTTG
GCTGCAGACACAGTGCTGGCCACACTGTATGCTGCCAAAAAGTACATAGTTCCTCACCTCGCCAGAGCCT
GTGTTAATTTCCTGGAGACCAGCCTAAGCGCCAAGAACGCCTGCGTACTCCTCTCCCAGAGCTGCCTGTT
TGAGGAGCCGGACCTGACACAGCGTTGCTGGGAGGTGATTGATGCCCAGGCCGAGTTAGCGCTCAAGTCT
GAGGGATTCTGTGACATCGACTTCCAGACACTCGAAAGTATCCTCCGCAGGGAAACGCTGAATGCCAAAG
AAATAGTGGTTTTTGAGGCAGCCCTGAACTGGGCTGAGGTAGAATGCCAGCGGCAAGATCTGGCCCTGAG
CATTGAAAATAAACGCAAGGTCCTAGGAAAGGCCCTGTACTTGATCCGCATCCCCACCATGGCCCTGGAT
GACTTTGCAAATGGTGCTGCACAGTCTGGGGTATTGACTCTCAATGAGACCAATGACATCTTCCTCTGGT
ACACTGCATCCAAAAAACCAGAGCTGCAGTTTGTGAGTAAAGCCCGCAAGGGCCTGGTCCCCCAGCGTTG
CCACCGATTTCAGTCATGTGCCTATCGGAGCAACCAGTGGCGCTATCGGGGTCGCTGTGACAGCATCCAG
TTTGCTGTTGATAAGAGGGTGTTCATCGCAGGCTTTGGGCTGTACGGCTCCAGCTGTGGCTCTGCCGAGT
ACAGCGCAAAGATCGAACTCAAACGGCAGGGCGTTGTCCTGGGCCAGAACTTGAGCAAGTACTTCTCAGA
CGGATCCAGCAATACCTTCCCTGTGTGGTTTGAGTATCCAGTGCAGATTGAGCCGGACACCTTCTACACA
GCCAGTGTAGTGCTGGACGGCAATGAACTCAGCTACTTTGGGCAGGAAGGCATGACGGAAGTTCAGTGTG
GCAAGGTGACTGTCCAGTTCCAGTGCTCCTCAGACAGCACCAATGGCACTGGTGTACAGGGAGGGCAGAT
CCCCGAACTCATATTCTACGCCTGAACTTTTCTTCTTTTATGCAGCATGAGGTGCCCACATGGGCCCTCC
TTGCCTGACGCTTAGCTCATCTGCAGATATGTGGTCAGCACAGGTAATTTGTAATGAATGAAGCAGTAGG
CAGTTTCTAATGTCTTTGAACCTTTTAATTATGTACAGGCTAAAATGCAACATTCCGCTTTTAACTCTAT
GCTTAGAAGAGCCAAGTTCTTACTCAGGCTTTGTGGGTTTCGAATTGCGTCTGTACGCCTAGGGAGATCG
TGTGCTTTTCCAAGTGCCCACCAACCTCCTACTTCTGTACCTCTGAAGAAGATTTTGGATCAACTTGAAT
TTCCTTTGATGAATGGAATTATGTATCCTAAAGTAGTATCATTATTTTTAAACAGAACCCCTTTCTCTCA
ATAAATAAATAAAAGCAAAAACAAAGAACAGGTAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4011475 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4011475 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11363 same embryo
 EurExpress:euxassay_006662 same experiment
 MGI:4823514 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS