EMAGE mouse gene expression spatial database

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EMAGE:11372

Lrrc33 leucine rich repeat containing 33 ( MGI:2445095)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006774. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006774_01	euxassay_006774_02	euxassay_006774_03	euxassay_006774_04

euxassay_006774_05	euxassay_006774_06	euxassay_006774_07	euxassay_006774_08	euxassay_006774_09

euxassay_006774_10	euxassay_006774_11	euxassay_006774_12	euxassay_006774_13	euxassay_006774_14

euxassay_006774_15	euxassay_006774_16	euxassay_006774_17	euxassay_006774_18	euxassay_006774_19

euxassay_006774_20	euxassay_006774_21	euxassay_006774_22	euxassay_006774_23	euxassay_006774_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11372_wholemount_strong.wlz

11372_wholemount_moderate.wlz

11372_wholemount_weak.wlz

11372_wholemount_possible.wlz

11372_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11372_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

moderate

spotted

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

thymus primordium

strong

homogeneous

strong expression: see section 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7360
Entity Detected:	Lrrc33, leucine rich repeat containing 33 ( MGI:2445095)
Sequence:	sense strand is shown >T7360 CTGATGCCCCAGAGCGAGTAGGCAGCGCAGCAGCGGCAAGTGCTGTCCAGGAGGCTGGGGCCCTGGAGAC GATGCGCCCCGCGGAGCCGCCTGGGCCTGCGGGAGCAAGCTGTGCCTGAGATGGAGTTCCCGCCCCTCTG GCTTTGCCTGGGTTTTCACTTCTTGATTGTGGAATGGAGGAGCGGACCTGGGACAGCCACTGCAGCTTCC CAAGGAGGCTGCAAGGTGGTCGACGGAGTCGCTGACTGCCGGGGTCTGAACCTTGCTTCGGTACCCAGCA GCCTCCCACCCCATTCCCGGATGCTCATTCTGGATGCTAACCCTCTCAAGGACCTGTGGAACCATTCCCT CCAGGCCTACCCACGCCTGGAGAACCTCAGCCTGCATAGCTGTCACCTGGACCGTATCAGCCACTACGCC TTCCGAGAGCAAGGCCACTTACGCAACCTGGTTCTGGCAGACAACCGCCTCTCGGAGAACTACAAGGAGT CAGCCGCAGCACTCCACACCCTGCTGGGACTTCGGAGACTGGACTTGTCTGGAAACTCCCTGACCGAAGA CATGGCGGCTCTCATGCTTCAGAACCTCTCCTCGTTGGAGGTTGTGTCCTTGGCAAGGAATACCCTCATG AGGCTCGACGACTCTATCTTTGAGGGCCTGGAACACCTTGTAGAGCTAGATTTGCAGAGAAACTACATCT TTGAGATCGAGGGCGGGGCGTTTGACGGCTTGACTGAGCTGAGGCGTCTCAACCTTGCCTATAACAACCT CCCCTGCATTGTGGACTTCAGCCTCACCCAGTTACGGTTCCTCAACGTCAGTTATAACATCCTAGAGTGG TTCCTGGCAGCCAGGGAGGAGGTGGCCTTCGAATTGGAGATACTGGACCTGTCTCACAACCAGCTGCTCT TCTTCCCACTCTTGCCTCAGTGCGGCAAGCTGCACACCCTCCTGCTCCAGGACAACAACATGGGCTTCTA CAGGGAGCTTTACAACACCTCCTCCCCGCAGGAGATGGTGGCCCAGTTCCTTCTTGTGGACGGCAATGTG ACTAACATCACAACTGTCAACCTCTGGGAGGAGTTTTCCTCCAGCGACCTGTCAGCTCTTCGCTTCCTGG ACATGAGCCAAAACCAGTTCCGGCACTTGCCGGATGGTTTCCTGAAGAAGACACCTTCCCTTTCCCACCT GAACCTCAACCAGAACTGCCTGAAGATGCTCCACATCCGGGAGCACGAGCCGCCGGGAGCACTCACCGAG CTGGATCTGAGCCACAACCAGTTGGCAGAGCTGCACCTGGCTCCGGGGCTCACGGGCTCCCTCAGGAACC TCCGGGTGTTCAACCTGAGTTCCAACCAGCTCCTGGGGGTCCCCACTGGCCTTTTCGACAATGCCAGCAG CATCACTACAATCGACATGAGTCACAATCAGATCTCACTTTGTCCTCAGATGGTGCCCGTAGACTGGGAG GGGCCTCCCAGTTGTGTGGATTTCAGAAACATGGGCTCTTTGAGGAGCCTTTCTCTGGACGGCTGTGGGC TGAAGGCATTACAAGACTGCCCGTTCCAGGGGACCTCCCTCACTCATTTAGACCTGTCTAGCAACTGGGG GGTTCTGAATGGGAGCATCAGCCCTCTCTGGGCTGTTGCCCCGACATTACAGGTCCTGTCTCTCAGGGAC GTGGGCCTTGGTTCTGGCGCTGCAGAGATGGACTTCTCTGCGTTTGGGAATCTGCGGGCGTTGGATCTGT CGGGAAACTCCCTGACCAGCTTCCCAAAGTTCAAGGGCAGTTTGGCCCTTCGGACTCTCGACCTCCGCAG AAACTCTCTCACGGCCCTCCCTCAGAGGGTTGTGTCCGAGCAGCCTCTGAGGGGTCTGCAGACCATCTAC CTCAGCCAGAACCCTTATGACTGCTGTGGGGTGGAAGGATGGGGGGCCCTGCAGCAGCACTTCAAGACTG TTGCGGACTTGTCCATGGTCACTTGCAACCTCTCTTCCAAGATCGTCCGTGTGGTGGAGCTGCCCGAAGG CCTGCCTCAGGGCTGTAAGTGGGAACAGGTGGACACTGGTCTCTTCTACCTCGTGCTCATCCTGCCCAGC TGCCTCACCCTGCTGGTGGCCTGTACTGTCGTCTTCCTCACTTTTAAGAAGCCTTTGCTTCAGGTCATCA AGAGCCGCTGCCACTGGTCCTCCATATACTGACCCGTGTGCCAAGGCTAGAGACTTGGTTTTTCCTCGAG GATGCGTCTCTCCGCTGGATCTTTACTTTTGCAGGGGTCGAGTGTGATGCATTGAAGGTTAAAACTGAAA TTTGAAAGAGTTCCATCCTCAGTCCCATTAACTTCTCCTCCCATCCGTGTGATTTATCCTCATTGTCCTG GTGAAATATTTATTAAACGACATTCTGTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4192329 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4192329 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11374 same embryo
	EMAGE:11371 same embryo
	EMAGE:11373 same embryo
	EMAGE:11370 same embryo
	EurExpress:euxassay_006774 same experiment
	MGI:4825991 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source