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EMAGE:11380

Upp1 uridine phosphorylase 1 ( MGI:1097668)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11380 EMAGE:11380 EMAGE:11380 EMAGE:11380 EMAGE:11380
"Pseudo-wholemount" of euxassay_006779. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006779_01 euxassay_006779_02 euxassay_006779_03 euxassay_006779_04
EMAGE:11380 EMAGE:11380 EMAGE:11380 EMAGE:11380 EMAGE:11380
euxassay_006779_05 euxassay_006779_06 euxassay_006779_07 euxassay_006779_08 euxassay_006779_09
EMAGE:11380 EMAGE:11380 EMAGE:11380 EMAGE:11380 EMAGE:11380
euxassay_006779_10 euxassay_006779_11 euxassay_006779_12 euxassay_006779_13 euxassay_006779_14
EMAGE:11380 EMAGE:11380 EMAGE:11380 EMAGE:11380 EMAGE:11380
euxassay_006779_15 euxassay_006779_16 euxassay_006779_17 euxassay_006779_18 euxassay_006779_19
EMAGE:11380 EMAGE:11380 EMAGE:11380 EMAGE:11380 EMAGE:11380
euxassay_006779_20 euxassay_006779_21 euxassay_006779_22 euxassay_006779_23 euxassay_006779_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11380Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11380_wholemount_strong.wlz
11380_wholemount_moderate.wlz
11380_wholemount_weak.wlz
11380_wholemount_possible.wlz
11380_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11380_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 13 14 17 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8176
Entity Detected:Upp1, uridine phosphorylase 1 ( MGI:1097668)
Sequence:sense strand is shown

>T8176
TTTACAACAGCCTAGCTCTGCATTCTTGACCACGTCCACACGGCCATTCATCTCTGGTCTTGACGAAGTG
ATTGACTGGTGGTCTCTGACACTCTGGAAGCCTTCTCGCGACGTGGACATCGGTCTCTGACAGTCCTCCT
ACAGTGAGAACATGGCAGCTACAGGAACTGAAGCAAAGGACCTTGAAAACCATCACAATGACTGCTTCAT
CCAACTTTCAAATCCAAACATAGCAGCAATGAAGGAAGACGTGCTCTACCATTTCAACCTCAGCACTAGC
ACACACGATTTCCCTGCTATGTTTGGAGACGTGAAGTTTGTGTGTGTTGGTGGAAGCTCTTCCCGGATGA
ACACCTTCATCAAGTATGTGGCTGCAGAGCTGGGCCTTGACCATCCAGGGAAGGAATATCCCAACATCTG
TGCAGGCACTGACCGCTACGCCATGTATAAAGCCGGGCCCGTGCTGTCTGTGAGCCATGGCATGGGCATT
CCTTCCATCGGCATCATGTTGCACGAGCTCATCAAGATGCTGTACCATGCCAGGTGCTCCAACATCACCA
TCATCCGCATTGGCACTTCAGGCGGGATAGGTCTGGAGCCTGGCTCTGTTGTCATCACGCAGCAGGCAGT
GAATGAGTGCTTCAAGCCGGAGTTTGAGCAGATTGTCCTGGGAAAACGGGTGATTCGCAACACCAACCTG
GACGCGCAGCTGGTTCAGGAGTTGGTGCAGTGCTCCTCAGACCTGAATGAGTTCCCCATGGTTGTGGGCA
ACACCATGTGCACCTTGGACTTCTATGAGGGGCAAGGCCGTCTGGATGGCGCCCTCTGCTCCTACACAGA
GAAAGACAAACAGTCTTATCTGCGTGCAGCCCATGCCGCGGGTGTCCGAAATATCGAGATGGAATCTTCG
GTGTTTGCCACCATGTGCAGTGCCTGTGGCCTGAAAGCGGCTGTGGTGTGTGTCACCCTTCTGGACCGTC
TGCAAGGAGACCAGATCAACACTCCCCACGATGTGCTTGTGGAATACCAGCAGAGACCACAGCGGCTAGT
GGGCCACTTCATCAAGAAGAGCCTGGGACGGGCCTGAGCCCTGTGCTTTTCCGCCCCGGAGAAGCCTCTG
CAACTCCTGGGCAATAAATCTATTTTTTCATAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5144694 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5144694 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11384 same embryo
 EMAGE:11385 same embryo
 EMAGE:11383 same embryo
 EMAGE:11382 same embryo
 EMAGE:11381 same embryo
 EurExpress:euxassay_006779 same experiment
 MGI:4829112 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS