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EMAGE:11414

Tox thymocyte selection-associated high mobility group box ( MGI:2181659)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11414 EMAGE:11414 EMAGE:11414 EMAGE:11414 EMAGE:11414
"Pseudo-wholemount" of euxassay_006612. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006612_01 euxassay_006612_02 euxassay_006612_03 euxassay_006612_04
EMAGE:11414 EMAGE:11414 EMAGE:11414 EMAGE:11414 EMAGE:11414
euxassay_006612_05 euxassay_006612_06 euxassay_006612_07 euxassay_006612_08 euxassay_006612_09
EMAGE:11414 EMAGE:11414 EMAGE:11414 EMAGE:11414 EMAGE:11414
euxassay_006612_10 euxassay_006612_11 euxassay_006612_12 euxassay_006612_13 euxassay_006612_14
EMAGE:11414 EMAGE:11414 EMAGE:11414 EMAGE:11414 EMAGE:11414
euxassay_006612_15 euxassay_006612_16 euxassay_006612_17 euxassay_006612_18 euxassay_006612_19
EMAGE:11414 EMAGE:11414 EMAGE:11414 EMAGE:11414 EMAGE:11414
euxassay_006612_20 euxassay_006612_21 euxassay_006612_22 euxassay_006612_23 euxassay_006612_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11414Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11414_wholemount_strong.wlz
11414_wholemount_moderate.wlz
11414_wholemount_weak.wlz
11414_wholemount_possible.wlz
11414_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11414_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21 22 23 24
forearm mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02
forelimb digit 1 phalanx
moderate moderate
regionalmoderate expression: see section 04 05 21 22
forelimb digit 2 phalanx
moderate moderate
regionalmoderate expression: see section 04 05 21 22
forelimb digit 3 phalanx
moderate moderate
regionalmoderate expression: see section 04 05 21 22
hindlimb digit 1 metatarsal
moderate moderate
regionalmoderate expression: see section 04 05 24
hindlimb digit 1 phalanx
moderate moderate
regionalmoderate expression: see section 05 24
hindlimb digit 2 metatarsal
moderate moderate
regionalmoderate expression: see section 04 05 24
hindlimb digit 2 phalanx
moderate moderate
regionalmoderate expression: see section 05 23 24
hindlimb digit 3 metatarsal
moderate moderate
regionalmoderate expression: see section 05 24
hindlimb digit 3 phalanx
moderate moderate
regionalmoderate expression: see section 05 06 23 24
hindlimb digit 4 metatarsal
moderate moderate
regionalmoderate expression: see section 05 06 24
hindlimb digit 4 phalanx
moderate moderate
regionalmoderate expression: see section 06 07 23 24
hindlimb digit 5 metatarsal
moderate moderate
regionalmoderate expression: see section 06 24
hindlimb digit 5 phalanx
moderate moderate
regionalmoderate expression: see section 07 23 24
foot mesenchyme
moderate moderate
regionalmoderate expression: see section 04 06 07 24
lower leg mesenchyme
moderate moderate
regionalmoderate expression: see section 04 05
upper leg mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02 03 04
femur
moderate moderate
spottedmoderate expression: see section 22 weak expression: see section 08
thymus primordium
weak weak
regionalweak expression: see section 10 11 12 14 15
diencephalon
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17
vibrissa
weak weak
regionalweak expression: see section 04 05 21 22 23
telencephalon
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
hindbrain
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
midbrain
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18
spinal cord mantle layer
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14 15 16 17 18
cervico-thoracic ganglion
strong strong
regionalstrong expression: see section 09 10 15 16
cervical ganglion
strong strong
regionalstrong expression: see section 08 16
thoracic ganglion
strong strong
regionalstrong expression: see section 13 moderate expression: see section 14 15
inner ear
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 17 18 19 moderate expression: see section 20 21 22
neural retina
strong strong
regionalstrong expression: see section 01 02 03 22 23 24
nasal septum
moderate moderate
regionalmoderate expression: see section 12 13 14 15 16 17
vomeronasal organ
moderate moderate
regionalmoderate expression: see section 12 13 14 15
stomach
weak weak
regionalweak expression: see section 08 09 10 11
lower lip
moderate moderate
regionalmoderate expression: see section 07 08 19 20
mandible
moderate moderate
regionalmoderate expression: see section 02 03 10 11 12 15 16 17 22 23 weak expression: see section 04 05 08 09 18 19 20 21
upper lip
moderate moderate
regionalmoderate expression: see section 07 08 19 20
metanephros
moderate moderate
regionalmoderate expression: see section 21 weak expression: see section 08 09 10 11 17 18 19 20
genital tubercle of male
moderate moderate
regionalmoderate expression: see section 13 14 15 16 17
cricoid cartilage
moderate moderate
regionalmoderate expression: see section 10 11 14 15
thyroid cartilage
moderate moderate
regionalmoderate expression: see section 11 12 14
lung
weak weak
regionalweak expression: see section 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 23 24
axial skeleton
moderate moderate
regionalmoderate expression: see section 07 08 13 14 15 16 17 18 weak expression: see section 12
exoccipital bone
moderate moderate
regionalmoderate expression: see section 01 21 22 23 24
vault of skull
moderate moderate
regionalmoderate expression: see section 24
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 01 12 13 14 21 22 23 24
viscerocranium
moderate moderate
regionalExpression in the turbinate bone.
clavicle
weak weak
regionalweak expression: see section 06 07 09 10 17 18 19
scapula
moderate moderate
regionalmoderate expression: see section 01 24 weak expression: see section 23
pelvic girdle skeleton
moderate moderate
spottedmoderate expression: see section 19 20 21 weak expression: see section 09 10 11 12
axial skeleton tail region
moderate moderate
regionalmoderate expression: see section 12 13 14 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7942
Entity Detected:Tox, thymocyte selection-associated high mobility group box ( MGI:2181659)
Sequence:sense strand is shown

>T7942
CGGATGTGAGGGAAGGAAGGTGTTTCTTTTACTCCTGAGCCCAGACGCCTCTCTGTTCCGTCTGAGCTTT
TTTTTTTTTTTCTGAGCACTTTTTTTTTTTTTTTTTTTCAAAAAGGAAAAGAAAAGGAGTTGCTTGATGT
GAGAGTGAAATGGACGTAAGATTTTATCCTCCTCCAGCCCAGCCCGCTGCTGCGCCCGCCGCTCCTTGTC
TGGGACCTTCTCCCTGCCTGGACCCCTACTATTGCAACAAGTTTGACGGGGAGAACATGTATATGAGCAT
GACAGAGCCGAGCCAGGACTATGTGCCAGCCAGCCAGTCCTACCCTGGACCAAGTCTGGAAAGTGAAGAT
TTTAACATCCCACCCATCACGCCTCCCTCCCTCCCTGACCACTCTCTGGTGCACCTGAATGAGGTAGAAT
CCGGTTACCACTCTCTGTGTCACCCCATGAATCACAATGGCCTGCTCCCGTTCCATCCACAAACCATGGA
CCTGCCAGAGATCACGGTGTCCAACATGCTGGGCCAAGATGGCGCACTGCTCTCCAATTCCATCTCTGTG
ATGCAAGATATTGGGAATGCAGAAGGAGCTCAGTACAGCTCCCATCCTCAGATGGCAGCCATGAGGCCGA
GAGGCCAGCCTACAGACATCAGACAGCAGGCAAGCATGATGCAGCCCGGCCAGCTGACCACCATCAACCA
GTCGCAGCTGAGCGCACAGTTGGGCTTGAACATGGGAGGAACCAATGTGGCCCACAACTCCCCTTCTCCA
CCCGGAAGCAAGTCTGCAACCCCTTCCCCATCCAGCTCAGTGCATGAGGATGAGTGTGAGGATGCCTCCA
AGATCAATGGAGGAGAGAAGCGGCCTGCCTCTGATATGGGGAAAAAACCCAAAACTCCCAAGAAGAAGAA
GAAGAAGGATCCTAATGAGCCCCAGAAGCCTGTGTCTGCCTATGCTTTGTTCTTTCGTGATACCCAGGCT
GCCATTAAGGGCCAGAATCCAAATGCTACTTTTGGTGAAGTATCGAAGATTGTGGCATCAATGTGGGATG
GCCTAGGAGAAGAACAGAAACAGGTCTATAAGAAGAAAACCGAGGCTGCAAAGAAGGAATACCTGAAGCA
GCTAGCAGCATACAGAGCCAGCCTTGTATCCAAGAGCTACACTGATCCTGTTGATGTTAAGACATCTCAG
CCACCCCAGCTGGTCAACTCAAAGCCGTCAGTATTCCATGGGCCCAGCCAGGCCCACTCAGCTCTGTACC
TAAGTTCTCACTATCACCAACAACCAGGAATGACTCCTCAGCTCACCGCCATGCATCCGAGCCTCCCCAG
AAACATAGCACCTAAGCCCAATAACCAAATGCCAGTGACTGTCTCCATAGCCAACATGGCTGTGTCCCCA
CCACCGCCCCTTCAGATCAGCCCACCTCTTCATCAGCATCTCAGCATGCAGCAGCATCAGTCGCTTGCCA
TGCAGCAGCCCCTTGGGAGTCAGCTCCCCATGCAGGTCCAGACTGCCTTACACTCGCCCACCATGCAGCA
AGGATTTACTCTTCAACCCGACTATCAGACTATTATCAACCCTACATCTACAGCTGCACAAGTTGTCACC
CAAGCGATGGAGTACGTGCGTTCTGGGTGCAGAAATCCGCCCCCACAGCCCGTGGACTGGAGTACCGACT
ACTGCAGTAGTGGGGGCATGCAGAGGGACAAAGCGCTGTATCTCACCTGAAAATCTCGACACCTCTTCTT
TCCACTGAGGAGTTCAGGGAAATCTTTTCACCATGGATTGCTTTGCGCACTCAAAGCAGTTCTTCATTTG
ATTAGAAAATACAAGTTACTAAGCACTTAGGACCATGTGAGCTTGTGGGTCACCCACGCTGGAAGAAATA
GTCATGCTTCTTTATTATTTTTTTAATCTTTTATGGACATTGTTCCTCTTCCCAGAAGGAAGTGTGGACC
ATTCTGATTTCTGTTGGTTTTGCTGTGAAGTGCTGCGGCTCTAGTAACTGCCTTAGCAACGGTAGATGTC
TGGGGTAAACGGTCCTGGGTTTTCCATTGGTTTTCATAATGGGTGTTTATATGAAACTACTAAAGACTTT
TTAAATGGCTTGATGTAGCAGTCATAGCAAGTTTGTAAATAGCATCTATGTTACACTCTCCTAGAGTATA
AAATGTGAATGTTTTTGTAGCTAAATTGTAATTTGAAACTGGCTCACCCCAGTTTGTTGATTTCACAGAG
GGATTAAAATTGGCAAACATTCATATTTTTACTTCATTTTTAAAAACAACTTACTAATAGTTCTATATTT
TCAAAATACTTGAAAAAATATTCCCAAATGATTTTAATTTAAAAACAATTTGGCTTTGTCTTATTGATCA
GACAAAAAAGAAGCTAGTATGGAGGGAAGCGCAAACACATTTATTTTGTATTGCAGAAAAAAAAATCCTT
TTTTGTATCACTTTTGTGTAATGATTAGTAAATGTCATCCAAGTCCTGTTATGGATAAAGCTGCCAAATG
CTCACTGGCATCTTAGCTGCAGAAACAGGCCGGAACGCGCTAGCTAAGTGCTCCTCTTTATCTATGGCTC
TGTGTTACTGTTTCTCCATACTGTGTCTGTATTTAATCGTTTTCTTCTTTTTTTTATGAAACTTGTTGCG
CCTATTTATGAAATAATAAATATAGGTGTTTGTAAGTAAATTTGTTAGTATTTGAAAGAGGTTTCATTGA
TGTTTTAATTTTTGCTGGCAAAAAAAAAAAAAAAAATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6518356 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6518356 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11415 same embryo
 EMAGE:11418 same embryo
 EMAGE:11417 same embryo
 EMAGE:11416 same embryo
 EurExpress:euxassay_006612 same experiment
 MGI:4828871 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS