EMAGE mouse gene expression spatial database

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EMAGE:11417

2310034C09Rik RIKEN cDNA 2310034C09 gene ( MGI:2152338)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006710. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006710_01	euxassay_006710_02	euxassay_006710_03	euxassay_006710_04

euxassay_006710_05	euxassay_006710_06	euxassay_006710_07	euxassay_006710_08	euxassay_006710_09

euxassay_006710_10	euxassay_006710_11	euxassay_006710_12	euxassay_006710_13	euxassay_006710_14

euxassay_006710_15	euxassay_006710_16	euxassay_006710_17	euxassay_006710_18	euxassay_006710_19

euxassay_006710_20	euxassay_006710_21	euxassay_006710_22	euxassay_006710_23	euxassay_006710_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11417_wholemount_strong.wlz

11417_wholemount_moderate.wlz

11417_wholemount_weak.wlz

11417_wholemount_possible.wlz

11417_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11417_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

forearm rest of mesenchyme

strong

regional

strong expression: see section 01 02 03

shoulder rest of mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05

upper arm rest of mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 21 22 23 24

hand

strong

regional

strong expression: see section 02 04 23 24

tarsus rest of mesenchyme

strong

regional

strong expression: see section 06 07 08 23 24

lower leg rest of mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05

upper leg rest of mesenchyme

strong

regional

strong expression: see section 03 04 05 06 07 08 09 21 22 23 24

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

tongue extrinsic muscle

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17

tongue intrinsic muscle

strong

regional

strong expression: see section 11 12 13 14 15 16 17

tail paraxial mesenchyme

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7966
Entity Detected:	2310034C09Rik, RIKEN cDNA 2310034C09 gene ( MGI:2152338)
Sequence:	sense strand is shown >T7966 GCTGTTAACATCCAACCCTCAGCCTCCTCTTCAACATGGCCTACAGCTGCTGCTCTGGAAACTTCTCCTC CCGCTCCCTTAGGAGCTGCCTGCCCTCCTCAGGATCCTGCCGTGGCTCTTCCTACCCCAGCAACCTGGTC TACACCACTACCTCCTGTTCTCCCAGCACCTGCCAGCTGAGCTCCACTCTGAACCCTGGCTTCCAGGAGA CCTGCATTGAGCCCATCCGCTGTCAGGAGACCTGCATTGAGCCCATCCGATGCCAGGAGACCTGCACTGA GCCCATCCGCTGCCAGGAGAGCTGCATTGAGCCCATCCGCTGCCAGGAGACCTGCATTGAGCCCATTAGC TGCCAGAGGTCCTGTGTGGTGCCCAGTTCCTGCCAGAAGCCCTGCTACTATCCCAGGAGCTCCACACCCT ACCGTCCCTGCCAGGGGACATATGCTGGCTCTCTGGGCTTTGGGTCCAGAAGCTGCAGCTCCCTGGCCTA TGGATCTAGAAGCTGCTACTCAGTGGGGTATGGAAATAGTGGCTTCAGACCCCTCAATTGTGGAGTCTAT GGCTTCCCTTCCTTGAGTTATGGGTCCAGATATTGCTCTCCAATCTATTTTGCTTCTCGATCCTGCCAAC CTTGTTATAGACCAACCTGGGGATCAGGCCTCTGCGGCATCAGCTGTTAAGTCTTTAACTCTCCTATGTC TGTCTATACTCTGAAGCTATTGTTTTCCTGATTAATCACTACAGATAGCCTACCATCCTACCATTAATGG CTTTTTCTTCTCAAAAATTGGGCAGATTATGAGATTAGTTGGTTATCCAGAAATTATGCTTTACTCTCTG TACTATTGATATAGACACATTCTATTTTATTTCAACCCAAGGTCATTTGAAAATTGAAAATTCCATCCAA TAAACTTGTGCAATCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30287188 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30287188 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11415 same embryo
	EMAGE:11414 same embryo
	EMAGE:11418 same embryo
	EMAGE:11416 same embryo
	EurExpress:euxassay_006710 same experiment
	MGI:4822664 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source