EMAGE mouse gene expression spatial database

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EMAGE:11431

Rcl1 RNA terminal phosphate cyclase-like 1 ( MGI:1913275)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006608. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006608_01	euxassay_006608_02	euxassay_006608_03	euxassay_006608_04

euxassay_006608_05	euxassay_006608_06	euxassay_006608_07	euxassay_006608_08	euxassay_006608_09

euxassay_006608_10	euxassay_006608_11	euxassay_006608_12	euxassay_006608_13	euxassay_006608_14

euxassay_006608_15	euxassay_006608_16	euxassay_006608_17	euxassay_006608_18	euxassay_006608_19

euxassay_006608_20	euxassay_006608_21	euxassay_006608_22	euxassay_006608_23	euxassay_006608_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11431_wholemount_strong.wlz

11431_wholemount_moderate.wlz

11431_wholemount_weak.wlz

11431_wholemount_possible.wlz

11431_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11431_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

strong

regional

strong expression: see section 04 05 06 22 23 24 moderate expression: see section 07 20

thymus primordium

moderate

regional

moderate expression: see section 12 13 14 15 16 17

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 10 11 18 19 20 21 22

pancreas

moderate

regional

moderate expression: see section 10 11 12 17 18 19

vibrissa

moderate

regional

moderate expression: see section 07 08 09 22 23

hindgut

moderate

regional

moderate expression: see section 17 18 19 weak expression: see section 15 16

rectum

weak

regional

weak expression: see section 15

midgut

moderate

regional

moderate expression: see section 17 18 19 20 21 weak expression: see section 08 09 10 11 12 13 14 15 16

liver lobe

strong

regional

strong expression: see section 11

liver left lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

liver right lobe

strong

regional

strong expression: see section 13 14 15 16 17 18 19 20 21 22 23 24

renal cortex

moderate

regional

moderate expression: see section 07 08 09 10 11 12 17 18 19 20 21 22

testis

moderate

regional

moderate expression: see section 06 07 21 22 23 weak expression: see section 08 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8087
Entity Detected:	Rcl1, RNA terminal phosphate cyclase-like 1 ( MGI:1913275)
Sequence:	sense strand is shown >T8087 AAGAGGTAATCCCGCGTCGGGGGCTGCCTTGGTCCTGGTCGCGCTCCCGGGTCCCGGGTCCCGGGCCTCT GCGGACGGCGCGCACATGGCGACCCAGGCGCACTCACTCAGCTACGCGGGGTGTAACTTCTTGCGCCAGC GGCTGGTCCTGTCCACCCTGAGCGGGCGACCGGTCAAGATCCGCAGGATTCGCGCCAGGGACGACAACCC GGGCCTCCGAGATTTTGAAGCAAGCTTTATAAGGCTATTGGATAAAATAACCAATGGTTCTCGAATTGAA ATCAACCAAACAGGAACAACCTTATACTACCAGCCTGGCCTCCTGTATGGCGGGTCTGTGGAGCACGACT GCAGTGTCCTCCGTGGCATTGGCTATTACCTGGAAGCTCTGCTTTGCCTGGCTCCGTTTATGAAACACCC ATTAAAAATCGTTCTGCGAGGAGTGACCAATGACCAGGTTGACCCTTCGGTTGATGTTCTTAAAGCGACA GCGCTCCCTCTACTGAAACAGTTTGGGATCGATGGAGAATCATTTGAGCTAAAGATCTTGCGTCGGGGAA TGCCCCCTGGAGGAGGGGGCGAAGTGCTTTTCTCCTGCCCCGTGAGGAAGGTCCTGAAGCCTGTCCAGCT CACAGACCCAGGAAAAATCAAGCGGATCAGAGGAATGGCGTACTCAGTTCGTGTATCACCTCAGATGGCA AACCGGATCGTGGATTCCGCAAGGAGCATCCTCAACAAGTTCATACCTGACATCTACATCTACACGGATC ACATGAAAGGCGTCAGTTCTGGGAAGTCTCCAGGCTTTGGGCTGTCGCTGGTTGCAGAGACTACCAACGG TACCTTCCTGAGTGCGGAACTGGCCTCCAATCCCCAGGGCCAGGGAGCAGCAGTGCTTCCCGAGGACCTC GGCAGGAACTGTGCAAAGCTGCTACTTGAAGAGATCTACAGGGGTGGATGCGTAGACTCGACCAACCAAA GTCTAGTTCTGCTGCTCATGACCCTTGGACAGCAGGACGTTTCCAAAGTCCTGCTGGGACCACTCTCACC CTACACAATTTTTGCGGCATCTGAAGAGCTTTTTCCAGGTTATGTTTAAAGTTGAAACCAAGCCATGTGG TGAAGAACTGAAGGGCGGGGACAAAGTGCTAATGACCTGCGTTGGTATTGGCTTCTCTAACCTGAGCAAG ACCCTCAAGTGATCCTGTACAACGTCAGGCTCTGCTGTGCATGGATGAAGCAGAAAGATGCGAAGGACCA CAGGGACCAAGTCCAGGCATGTCCATCCAGGACAGGCGGCCTCTTAACATTACAGACTTTCTTTTCCACT GAAAATACTGATTTGCAAATACAAGCTGTCTCTCCCACGTGACTTCCAGCTGCTTCTCCAATGACATTCC TCGGAGGCCCAGGCTGTGGGCTTCCAGACTCACCTGGAGGAGGGGCCCTCCGTGAGTGGCCACGCTGCTA GACAGTATCCCAGCAGCTCTTCTTCATCTTTGGATTGTGGCTCTGTGTAGGGTTTATGTGACAACGTGAC AGTGTGTGTAGAAGACCTCCAGTCTGTTGTGCCAGAAGTTGGGGCACTGGTTCCCTCTCTCGTCTTAGGA ATAATTTGCTTGCATGGGCACATGCTATACCTCACATGGTAACTTGTGTTTGTGTGGGCCTGGTGGTTGG GATGGGGAGGGCAGACATGTCTTAAATGTTTTCTGTATCCCAGGTATGGCATAAAATTTTGTTTTTATTC TTCTAATTGCACTGTAGTGTAAATCAGAGTATTTATTTTTCATTTGATGTTTATAATTCAGAAACATTAG ATCTGATTTAAAGATGTAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30059875 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30059875 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11434 same embryo
	EMAGE:11432 same embryo
	EMAGE:11433 same embryo
	EMAGE:11430 same embryo
	EMAGE:11429 same embryo
	EurExpress:euxassay_006608 same experiment
	MGI:4827665 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source