EMAGE mouse gene expression spatial database

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EMAGE:11435

Lrrtm3 leucine rich repeat transmembrane neuronal 3 ( MGI:2389177)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006601. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006601_01	euxassay_006601_02	euxassay_006601_03	euxassay_006601_04

euxassay_006601_05	euxassay_006601_06	euxassay_006601_07	euxassay_006601_08	euxassay_006601_09

euxassay_006601_10	euxassay_006601_11	euxassay_006601_12	euxassay_006601_13	euxassay_006601_14

euxassay_006601_15	euxassay_006601_16	euxassay_006601_17	euxassay_006601_18	euxassay_006601_19

euxassay_006601_20	euxassay_006601_21	euxassay_006601_22	euxassay_006601_23	euxassay_006601_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11435_wholemount_strong.wlz

11435_wholemount_moderate.wlz

11435_wholemount_weak.wlz

11435_wholemount_possible.wlz

11435_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11435_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foot mesenchyme

moderate

regional

moderate expression: see section 04 05 06 07 23 24

lower leg mesenchyme

moderate

regional

moderate expression: see section 03

upper leg mesenchyme

moderate

regional

moderate expression: see section 04

vibrissa

moderate

regional

moderate expression: see section 04 05 06 07 08 22 23 24

hypothalamus mantle layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 19 20 21 22 23 24 moderate expression: see section 13 15 16 17 18

telencephalon marginal layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 19 20 21 22 23 24 moderate expression: see section 13 17 18

telencephalon ventricular layer

strong

regional

strong expression: see section 06 20

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

rest of cerebellum mantle layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 16 17 18 19 20 21 not examined expression: see section 11 12

pons mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

midbrain mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

trigeminal v ganglion

strong

single cell

strong expression: see section 03 04 05 06 07 08 09 18 19 20 21 22 moderate expression: see section 23

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 08 18 19 20 21

ventral grey horn

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17

stomach

strong

regional

strong expression: see section 05 06 08 09 moderate expression: see section 10

midgut

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21

lower lip

strong

regional

strong expression: see section 08 09 10 11 12 19 20 21

mandible

moderate

regional

moderate expression: see section 06 07 17 18 19 21 23 24

upper lip

strong

regional

strong expression: see section 07 08 09 10 11 12 19 20 21 22 23

maxilla

moderate

regional

moderate expression: see section 23

palatal shelf

strong

regional

strong expression: see section 07 08 09 10 11 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8153
Entity Detected:	Lrrtm3, leucine rich repeat transmembrane neuronal 3 ( MGI:2389177)
Sequence:	sense strand is shown >T8153 CTAAGGACGACCTTTGAACAATACAAAGGATGGGTTTCAATGTAATTAGGCTACTGCGAGGATCAGCTGT AGCGGTGGTTCTAGCACCCACTGTCTTACTGACAATGCTTTCTTCTGCTGAACGAGGATGCCCTAAGGGT TGTAGGTGTGAAGGCAAAATGGTATACTGTGAGTCTCAGAAACTGCAGGAGATACCCTCGAGTATATCTG CCGGTTGCTTGGGTTTATCCCTTCGCTACAACAGCCTCCAAAAACTTAAGTATAATCAATTTAAAGGGCT CAATCAGCTCACCTGGCTCTACCTTGACCACAACCACATCAGCAATATCGACGAGAATGCATTCAATGGG ATACGCAGACTCAAAGAGTTGATTCTGAGTTCCAACAGAATCTCCTATTTCCTTAACAACACCTTCAGAC CTGTGACCAATTTACGGAACTTGGATCTGTCCTACAATCAGCTCCATTCTCTGGGATCGGAACAGTTCCG GGGCTTGAGGAAGCTGCTTAGTTTACACCTCCGCTCCAACTCACTGAGAACCATCCCGGTGCGGATCTTC CAAGACTGTCGCAACCTGGAACTTCTGGACCTGGGATACAACCGGATCCGAAGTTTAGCCAGGAATGTCT TTGCTGGCATGATCAGACTCAAAGAGCTTCACCTGGAGCACAATCAATTTTCCAAGCTCAACCTGGCCCT GTTCCCAAGGCTGGTGAGCCTTCAGAACTTGTACATGCAGTGGAATAAAATCAGTGTCATAGGGCAAACC ATGTCCTGGACCTGGAGTTCCTTACAGAGGCTCGACCTGTCTGGTAATGAGATCGAAGCCTTCAGCGGAC CCAGTGTCTTCCAGTGTGTTCCCAATCTGCAACGCCTCAACCTGGATTCCAACAAGCTCACATTTATTGG ACAAGAGATTCTGGATTCTTGGATCTCTCTCAATGACATCAGTCTGGCTGGGAATATATGGGAATGCAGC AGGAATATCTGTTCCCTGGTAAACCGGCTGAGAAGTTTTAAAGGTCTGAGAGAGAATACAATCATCTGCG CCAGTCCCAAAGAGCTGCAGGGGGTTAACGTGATCGATGCGGTGAAGAACTACAGCATCTGTGGGAAGAG CACTACCACAGAGAGGTTTGACCTGGCCAGGGCCCTCCCCAAGCCCACATTTAAGCCCAAGCTCCCCAGG CCGAAGCACGAGAGCAAGCCTCCTCTGCCCCCCACGGTGGGAGCTACCGAGCCCAGCCCAGAGACAGATG TGGACACGGAGCACATCTCCTTCCATAAGATCATCGCGGGCAGCGTGGCCCTTTTCCTGTCGGTGCTGGT CATCCTCCTGGTGATGTACGTGTCCTGGAAGCGGTACCCCGCGAGCATGAAGCAGCTGCAGCAGCGCTCC CTCATGCGAAGGCACCGGAAGAAGAAACGGCAATCGCTCAAGCAGATGACTCCAGGCACCCAGGAATTTT ATGTAGATTATAAACCCACCAACACGGAGACCAGCGAGATGCTGCTGAACGGAACGGGACCCTGCACCTA TAGCAAATCAGGCTCCAGGGAATGTGAGGTATGAACCATTGCGATTAAAAAATAATAATAATAAAGGAAG GAAGGAAGAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30044805 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30044805 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11439 same embryo
	EMAGE:11436 same embryo
	EMAGE:11437 same embryo
	EMAGE:11438 same embryo
	EurExpress:euxassay_006601 same experiment
	MGI:4826009 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source