EMAGE mouse gene expression spatial database

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EMAGE:11437

Gjb2 gap junction protein, beta 2 ( MGI:95720)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006599. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006599_01	euxassay_006599_02	euxassay_006599_03	euxassay_006599_04

euxassay_006599_05	euxassay_006599_06	euxassay_006599_07	euxassay_006599_08	euxassay_006599_09

euxassay_006599_10	euxassay_006599_11	euxassay_006599_12	euxassay_006599_13	euxassay_006599_14

euxassay_006599_15	euxassay_006599_16	euxassay_006599_17	euxassay_006599_18	euxassay_006599_19

euxassay_006599_20	euxassay_006599_21	euxassay_006599_22	euxassay_006599_23	euxassay_006599_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11437_wholemount_strong.wlz

11437_wholemount_moderate.wlz

11437_wholemount_weak.wlz

11437_wholemount_possible.wlz

11437_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11437_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

strong

regional

strong expression: see section 09 moderate expression: see section 10 11 12 15 16 17 18

epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vibrissa

strong

regional

strong expression: see section 03 04 05 06 07 08 21 22 23 24 weak expression: see section 20

diencephalon meninges

strong

regional

strong expression: see section 01 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

telencephalon meninges

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

hindbrain meninges

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

midbrain meninges

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

spinal cord meninges

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18

inner ear

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 16 17 18 19 20 21 22

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 02 03 04 07 08 09 17 18 20 21 22 23 24

eyelid

strong

regional

strong expression: see section 01 02 03 04 23 moderate expression: see section 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 13 16 17 18 19 20

oral epithelium

strong

regional

strong expression: see section 05 06 08 09 10 11 12 13 14 15 16 17 18

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 weak expression: see section 19 20 21 22 23 24

seminiferous cord

strong

regional

strong expression: see section 05 06 07 moderate expression: see section 08 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7402
Entity Detected:	Gjb2, gap junction protein, beta 2 ( MGI:95720)
Sequence:	sense strand is shown >T7402 CGGACGCGTGGGCGCAGCCAGTCGGCGGCGCTCCAGTCCACTGAGCGCAGCCTCCACCGGGACCCGCGGC GACCAGCACAGGATCAGCCGGCGACCCATTTCGGACCAACCCAGGAGCGGCCCCGGGACCCGCTTCAGAC CTGCTCCTTACCGGGGACAGCGCCTCGCCGCTTCCCGCCACCCAACGCTGCACGCTCCTCCGGACACAGT GCCAACCATCCAGAGGACAAGATGGATTGGGGCACACTCCAGAGCATCCTCGGGGGTGTCAACAAACACT CCACCAGCATTGGAAAGATCTGGCTCACGGTCCTCTTCATCTTCCGCATCATGATCCTCGTGGTGGCTGC AAAGGAGGTGTGGGGAGATGAGCAAGCCGATTTTGTCTGCAACACGCTCCAGCCTGGCTGCAAGAATGTA TGCTACGACCACCACTTCCCCATCTCTCACATCCGGCTCTGGGCTCTGCAGCTGATCATGGTGTCCACGC CAGCCCTCCTGGTAGCTATGCATGTGGCCTACCGGAGACATGAAAAGAAACGGAAGTTCATGAAGGGAGA GATAAAGAACGAGTTTAAGGACATCGAAGAGATCAAAACCCAGAAGGTCCGTATCGAAGGGTCCCTGTGG TGGACCTACACCACCAGCATCTTCTTCCGGGTCATCTTTGAAGCCGTCTTCATGTACGTCTTTTACATCA TGTACAATGGCTTCTTCATGCAACGTCTGGTGAAATGCAACGCTTGGCCCTGCCCCAATACAGTGGACTG CTTCATTTCCAGGCCCACAGAAAAGACTGTCTTCACCGTGTTTATGATTTCTGTGTCTGGAATTTGCATT CTGCTAAATATCACAGAGCTGTGCTATTTGTTCGTTAGGTATTGCTCAGGAAAGTCCAAAAGACCAGTCT AATGCATTGCCTGGCTGCTAGAGCAAAGATGGAGGGAGAGGATGAGGCAACCCATGCTTAGTCGCTTAGT CGGCAGAGCTCAGCCACCAGCAGTTCCCAAGACAAACATTCCCATCTAAAATGCCACCATTTGAAGTCCC TGGAGGCCTCCTATGAAACTCCAGAAGCCTCCGTGGGCCTCCCTTCCCCCAAAGCTCCCAAACAAAGGCC CAATTCTATGCCTGTATTAATGGGTTCTAAAGTTAGTTCCACTGAGACCCCGTGCTGGTAGCGACCAAGC TTGGAGGATACATTCACAGTTTAAACAAAGGGATCTCACATTGTTTCTCTTCCTCTGAGGACAGGAGAGA GGAACCCGGTCCTGAGGAAGGTGCCACTGAGAAAGTCCCCCCCCCCAAGTTACCCCTAGCTAAAGGTAAA GAAATGCCATACTGTTATTTGCTTTGATAGTTTACGTGTGCAACAATGGACAAAAAGCTATTAATACTTC AAGTGCTGTGTACTTTTTTTTTTTAATGAAAACCTTAAAATAAGATAGGCTCTTTTTTTTCCTAAAATGA TCTGGAAAGTATTATACATTCCTCCTAGTCCAGAGGTTCAGTGTGTGAAGTGAGCATGGTGTGTAACCAG ATCTCACAGGGTCTTTAAGATGTTGGCCTTTGGGTTATGGGAAACCTGCAGTGTGGCTGAGGGCCCACCT ATTGTATTCATCACTGGCGTGCTGGGCTCAGGGCTATAACCCTACTGCCTGTCATAAGTGAGACAAACTG GAAGCGTCTCGTGCTGGCTTCTGACAAGAAGAGGCCTCAATTAAAATAGTCAACTGTAATTTTGTGTAAG AGACAGATAGGATATGCCGACAACTGCCACCTGCCATGAGCCTAACTCAGCCCCACCCCACCCCCACCCC CCACCAGTTTGTCTACTCTGTAGCTGTGGGATGTTGCAGTCAGCATCAGAAGATAGGGACTTCATGGGTT TGCTTGGGAATTTCACTGCCATGGGACAATTCAATGGTGCAGAAACCAAATGGGGTGGTTTTCAGAGAAC CAATGAAACTTCTAGACTCCCAATCCTGTTGATTAAAACTAAGTTTTTCTACTTTTGAAAGTGTGTTTGC CTCCCTCTTCAGCATTGCCTTCTTAACTGGAAACAGAAATGTGGATATTTGGAAGAAATAGAAGAAACTA GTTTAGGTCAGTATGTAACCTTTCTAGGGCAAGTTGAACCATTACCACTATGCCTTATGTGTATCCTACA AGATGACAGTCAACAAATCCAACAGGGGACACTTCTTCCTGCCAAGAATGTCATTGGAAAGCCATTTTGT AACAATAAATAAGAGTTGTGGTTTCATGCTTGTCACTGATATAATTTTGTAAACCCCTAAATGCCCTTTA TTAAAATATGATTATTTTGTATACTTTAAAATTAAATAAGGTTTTTAATAGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4194757 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4194757 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11439 same embryo
	EMAGE:11436 same embryo
	EMAGE:11435 same embryo
	EMAGE:11438 same embryo
	EurExpress:euxassay_006599 same experiment
	MGI:4825060 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source