EMAGE mouse gene expression spatial database

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EMAGE:11439

C1qtnf1 C1q and tumor necrosis factor related protein 1 ( MGI:1919254)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006596. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006596_01	euxassay_006596_02	euxassay_006596_03	euxassay_006596_04

euxassay_006596_05	euxassay_006596_06	euxassay_006596_07	euxassay_006596_08	euxassay_006596_09

euxassay_006596_10	euxassay_006596_11	euxassay_006596_12	euxassay_006596_13	euxassay_006596_14

euxassay_006596_15	euxassay_006596_16	euxassay_006596_17	euxassay_006596_18	euxassay_006596_19

euxassay_006596_20	euxassay_006596_21	euxassay_006596_22	euxassay_006596_23	euxassay_006596_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11439_wholemount_strong.wlz

11439_wholemount_moderate.wlz

11439_wholemount_weak.wlz

11439_wholemount_possible.wlz

11439_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11439_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 16 20 21 22 23 24

heart valve

strong

regional

strong expression: see section 13 moderate expression: see section 11

heart ventricle

strong

spotted

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

lower jaw incisor

strong

regional

strong expression: see section 17

lower jaw molar

strong

regional

strong expression: see section 07 08 19 20

upper jaw incisor

strong

regional

strong expression: see section 16 17

upper jaw molar

strong

regional

strong expression: see section 07 20

axial skeleton

strong

regional

strong expression: see section 12 13 14 15 16

axial skeleton tail region

strong

regional

strong expression: see section 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7123
Entity Detected:	C1qtnf1, C1q and tumor necrosis factor related protein 1 ( MGI:1919254)
Sequence:	sense strand is shown >T7123 GAGCCCTGAGAGGCTGGAGGATTCCATTGCACAGCCGGCTCAGCTGGAAGGAGGAAGATGGGCTCCTGTG CACAGGGATTCCTGCTGGGATGCTGCCTGCTGCTGGCCATCACCTGGGGCCCCATCCTGAGCCTTGTGCC ACGAGTTCAGGAGGAACAACAGGAGTGGGAAGAGACAGAGGAGCTGCCATCTCCTCTGGATCCTGTGACA AGGCCTGAAGAAACACGAGAGAAGTATAGCCCTCGCCAGGGTGAGGACCTCCCCACTTCTCGGTGCTACC GATGCTGTGACCCCAGCACTCCTGTATACCAGACAATTCCTCCACCCCAGATCAACATCACCATCCTGAA AGGCGAAAAAGGTGACCGAGGGGATCGAGGCCTCCAGGGGAAGTACGGCAAAATAGGTTCTACAGGTCCT AGGGGCCATGTTGGCCCCAAAGGGCAGAAGGGATCCATTGGAGCCCCTGGGAACCACTGCAAGAGCCAGT ACGCAGCCTTCTCCGTGGGCCGGAAGAAGGCTTTGCACAGCAACGACTACTTCCAGCCCGTGGTCTTTGA CACGGAGTTTGTGAACCTCTACAAACACTTCAATATGTTCACTGGGAAGTTCTACTGCTATGTGCCAGGC ATCTACTTCTTCAGCCTCAACGTGCACACTTGGAACCAGAAGGAGACGTACCTGCACATCATGAAGAACG AGGAGGAGGTGGTGATCCTGTATGCGCAGGTGAGCGACCGCAGCATCATGCAGAGTCAGAGCCTGATGAT GGAACTGCGGGAGGAGGATGAGGTCTGGGTGCGTCTCTTCAAGGGCGAGCGTGAGAACGCCATTTTCAGT GACGAGTTCGACACCTACATCACCTTCAGTGGCTACCTGGTCAAGCCAGCCTCTGAGCCCTAGCGGACAC TCCTGTGGAGCTTTTGTGGACTGCTGACCTCCTTGCCTGGCACCCTGACCTATCCCTGCATTCTACAGAC ACTGGAGTCCTGCCCCGGGCTGACCCCATTTTCTCTCTGCTCCATCCTGGCTTCCTTGGCCTTGGCTTCC AAAGTTTTGGCTTTTGACAAGATGCCCTTGGCCACTGGGAATCCCAAAGGACGGTGCGATCCCAGATCTG GCTGCTACTCTAAGCAGAGAGCTGCTGGCAGATGAAATCATTGGGCGGGGAGCCTGTGAGGATATTGGGG GGCCTCCAGCTCCTTCTGTGTACACAGCCTTAGACGACCCTGTGCTGTGTTGTCCCGTGGCCACAGGGTG TTCCAGAGCACAGCCCCTGTGTGTTCCCATTCCTGGACGCAAGTAAGCAAATATCATGGGTTTCTTAGGA ACGAAGTCAAGCAGAAAAGAGAAAGAAAGGTGGTGTTAGTTTTGGCTTTCCAGCCAGCTGGAGGGAGGGA TGGGGAGAGAGAGAGCGAGAGCTATTTGTATTGGGGAAACTGAGGCATAGGAAAAACATGAATGGCAACA GAGTAGCTGCAGTTTGTGGGTTTGGAAACCACATCTGACTTAACTCTAGATCACATATGAGCTTTCCTGG GGACAGCAGGACTGACCTCCGAGCTCTGTTGACATGCTATAGCCTTGCCCAGGGGCTGGTCAATCTTTCT GAGCCACACTAGTAAAAGGGTTGGGGGAGAACAGCAAGTGCCCCCTGTGGTTGGCTCTGGGCTGGTGGCA GCATCCTGCTTGCCCCAACTCACAGGATCCTGACAGCAGCTGGGAACCTCAGGGACTCCTGCAGCTTTCT CTGTAAGAAATAAAGCTCCTACTATGTCCCAGTACCTCTCTGCTCTGCTCCACTTCCCCAGTCACTCTGG ACCCCAGGGTGGGAGGGCTCTCTTGCCTGTTGGGACATCAGTTCCCCTTCCTCCTTCTTGGTGAATTAAC CATGGAAGGACCAGGGCTCGGATTTGGGTTCCCAAACTGCCCTTCACCATCCCTAGTGTCCTGCTTCCTT CCCAGTTCAGCATCCTGTCTGGGAACTTGATACTTTAACCTGCTAGAGCTGATGAGTCTGATAGACCTGC CCAGCCCTGACACAGCCCTAGTCAGCTTATGGACACGTGAGAGGGACTTCCTTTGAGACCCAGAGCTGGA GTAGAGCTATAAAAATCTACCTATTCCCGGGTCAACCCCAAGTGGTAGAAGAGGACACAGGCTATCCCGC CCCTAGCTCAGACTCAGGGAAGGCCTCAGGCCTGATTGTCTGATTGCAGAGAGTCTGTGTTCTTTCCCCA TCTCACCCCGTGTTGATCCCCAGGGCCTGGGCCACTGGATATCTGCTTTGTGCCAACTAGGCCTTGCTTG CTGCTTCCTGGTGGCCCTTGGTTAGGATCCCTCTCTTTTCCTTCTGGAGCTCAATGTACGTATATGCCAC CTCCGAAGGGGCTTCTGCTGGTCAGACTCTCCAAGCCACTTCCATGGGCATGCCTACAGCAGAGGCTGCT GCCTCCTGTGCTCTACCCTGCTCTTTCCAGAAAACATTAAACTTGCCATGGCGATTCACAGCAAAAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4018549 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4018549 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11436 same embryo
	EMAGE:11437 same embryo
	EMAGE:11435 same embryo
	EMAGE:11438 same embryo
	EurExpress:euxassay_006596 same experiment
	MGI:4823537 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source