EMAGE mouse gene expression spatial database

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EMAGE:11452

Fbxo9 f-box protein 9 ( MGI:1918788)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006958. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006958_01	euxassay_006958_02	euxassay_006958_03	euxassay_006958_04

euxassay_006958_05	euxassay_006958_06	euxassay_006958_07	euxassay_006958_08	euxassay_006958_09

euxassay_006958_10	euxassay_006958_11	euxassay_006958_12	euxassay_006958_13	euxassay_006958_14

euxassay_006958_15	euxassay_006958_16	euxassay_006958_17	euxassay_006958_18	euxassay_006958_19

euxassay_006958_20	euxassay_006958_21	euxassay_006958_22	euxassay_006958_23	euxassay_006958_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11452_wholemount_strong.wlz

11452_wholemount_moderate.wlz

11452_wholemount_weak.wlz

11452_wholemount_possible.wlz

11452_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11452_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

strong

regional

strong expression: see section 05 moderate expression: see section 21 22 23

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 08 09 moderate expression: see section 20

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 10 moderate expression: see section 18 19 20 21 22 23

vagus x ganglion

strong

regional

strong expression: see section 09 moderate expression: see section 10 20

dorsal root ganglion

strong

regional

strong expression: see section 09 10 11 12 13 14 17 18 19 20

liver

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 weak expression: see section 01 02 03 04 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6817
Entity Detected:	Fbxo9, f-box protein 9 ( MGI:1918788)
Sequence:	sense strand is shown >T6817 GCAGAGCAGGCAGCTCCGCCGCTGCGTCCAGACGTCTCCTGCTGGGCCGACGCTCAGCTCTTCGCTCGGT GCTCGGCCTCCGCTGGCGACCGGCGACCCCTCCGCAGCTCGAGTCCCCGGCCGCCGCAGCGCGCCTGCCT CCGGCCCCCTGTCCCGCGAAAATGGCTGCCGTACGCCGGGCCCGCAGTTATTGCCGCTGCCTGGTGCGCT TCTCCGACCGAGAACTCTGCTAAGCCGCGCTGCAGCAGACGGGCAGGAGTAGACACCGGGCAGCCGCCAC CCCTCCGGGAGCCGGGCGCGGGAAGCCCCTCGACTGGGCCGGCCGCCGGCCAGCATGGCAGAAGCGGAGG AAGATTGTCATTCTGATGCTGACAGAGTAGGCGATGAAGGAAATGAGAGTCCGGCTGAGAGAGACCTGCA GGCGCAGCTCCAGATGTTCAGAGCTCAGTGGATGTTTGAACTGACCCCAGGTGTAGGCTCCAGTCATGGA GAAACTCGGCCTTGCAGAGCAGGAAGAAGCTCTATGCTGAAAGCAGCTGCAGACACCAAAGGACGACAGG AACTGGCAAAGGAAGAAAAGGCTCGAGAACTCTTCCTGCAGGCAGTGGAGGAAGAACAAAATGGAGCTCT CTATGAAGCCATCAAGTTCTACCGTAGGGCGATGCAGCTGGTGCCAGACATTGAGTTCAAGATCACTTAC ACCCGGTCTCCAGACGGCGACGGCGTTGGGAGCGGCTACATCGAAGAGAACGAGGACGCCAGCAAGATGG CCGATCTCCTGTCGTACTTCCAGCAGCAGCTCACGTTGCAGGAGTCTGTGCTCAAACTCTGTCAGCCTGA GCTTGAGACCAGTCAGACTCACATATCAGTCCTGCCTATGGAGGTGCTGATGTACATCTTCCGATGGGTG GTATCAAGTGACTTGGACCTCAGATCGTTAGAGCAGTTGTCACTGGTGTGCAGAGGATTCTATATCTGTG CCAGAGACCCTGAAATCTGGCGTCTGGCTTGCTTGAAAGTGTGGGGCAGAAGCTGCATGAAGCTCGTTCC ATACGCGTCCTGGAGAGAGATGTTTCTAGAACGGCCCCGAGTTCGGTTTGATGGAGTGTACATCAGTAAA ACCACGTATATTCGCCAGGGAGAGCAGTCACTTGATGGTTTCTACAGGGCGTGGCACCAAGTGGAATATT ACAGATACATGAGATTCTTTCCTGATGGCCATGTGATGATGTTAACCACCCCCGAGGAGCCTCCGTCCAT CGTTCCCCGGTTAAGAACCAGGAACACCAGAACGGATGCAATTCTGCTGGGTCATTACCGCTTGTCACAA GATGCAGACAATCAGACCAAAGTCTTTGCTGTAATAACTAAGAAAAAAGAAGAAAAGCCACTTGACCATA AATACAGGTATTTTCGTCGTGTTCCTGTCCAAGAGGCAGATCACAGCTTCCATGTGGGACTGCAGCTGTG CTCCAGTGGCCACCAGAGGTTCAACAAACTCATCTGGATCCACCACTCTTGTCACATCACTTACAAAGCA ACTGGTGAGACTGCAGTGAGTGCTTTTGAGATTGACAAGATGTACACGCCCCTGTTGTTCGCCAGAGTGA GGAGCTACACTGCCTTCTCGGAAAGGCCTCTGTAGAGCCACACCAGCATGTCACTGCTGCATGAGCAGAA GCATAGAGCGCTGGGACACCTAAGTTATATGTACATAGACACACGCATGTAGATAGACATGCATATATAC ATACATGGAGTTGGAGCTTACTTTAAGTTGTTGGAATTCCTTTAAGAAGAGTATAAATTATTTTTTTTAT TTAAAAGGATAGTTGATTCCTGGGGTACTTTGTTTTGAAATTAGAAGTTGTTAAGGACACTTTTGTTGTA ATAAATGTTGATGTAGAGTTGAAATCATGTTTTCAAGCAGGTTTCTGAGCAGATCTGTCACATCCACTGC CTTGTGCCTGAGCATCCTCAACACACATAGGTCAGGGAGCACACTGGAGAGTTCTTATTCCCGCATGACT TAGTGGCTTTGCATCATACTCAAATGTGAAACCTGTTCTGGATTTGTTTGAAACTGTTCAATAAAGATCA TAAATAAATGTTTCTTTCAAGGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3710611 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3710611 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4824814 same experiment
	EurExpress:euxassay_006958 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source