EMAGE mouse gene expression spatial database

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EMAGE:11467

Hspb2 heat shock protein 2 ( MGI:1916503)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006798. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006798_01	euxassay_006798_02	euxassay_006798_03	euxassay_006798_04

euxassay_006798_05	euxassay_006798_06	euxassay_006798_07	euxassay_006798_08	euxassay_006798_09

euxassay_006798_10	euxassay_006798_11	euxassay_006798_12	euxassay_006798_13	euxassay_006798_14

euxassay_006798_15	euxassay_006798_16	euxassay_006798_17	euxassay_006798_18	euxassay_006798_19

euxassay_006798_20	euxassay_006798_21	euxassay_006798_22	euxassay_006798_23	euxassay_006798_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11467_wholemount_strong.wlz

11467_wholemount_moderate.wlz

11467_wholemount_weak.wlz

11467_wholemount_possible.wlz

11467_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11467_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

strong

regional

strong expression: see section 01 02 03 04 05 06 07 24

upper leg muscle

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 19 20 21 22 23 24

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

forearm rest of mesenchyme

strong

regional

strong expression: see section 01 02 03 04

hand

strong

regional

strong expression: see section 05 moderate expression: see section 01 02 03 04

tarsus rest of mesenchyme

strong

regional

strong expression: see section 05 06 19 20 21

lower leg rest of mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 23 24

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

tongue muscle

strong

regional

strong expression: see section 12 13 14 15 16 17 18 19

tongue extrinsic muscle

strong

regional

strong expression: see section 11

tail paraxial mesenchyme

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8193
Entity Detected:	Hspb2, heat shock protein 2 ( MGI:1916503)
Sequence:	sense strand is shown >T8193 GGCTACTAGTCGCAACAGCAGTCATGTCGGGCCGCACAGTGCCACACGCCCACCCAGCCACTGCCGAGTA CGAATTTGCCAACCCCAGCCGTCTGGGCGAGCAGTGCTTCGGAGAAGGCCTCCTGCCAGAAGAGATCCTG ACCCCCACCCTCTATCACGGCTACTATGTTCGGCCTCGGGCCGCCAGAGCTGGCGAGGGCGCCAGGGCAG GGGCCTCAGAGCTCAGGCTCAGTGAAGGCAAGTTCCAGGCGTTTCTGGATGTGAGCCACTTTACCCCAGA TGAGGTGACGGTGAGGACTGTGGATAACCTGCTGGAGGTGTCTGCCCGACACCCCCAGCGTCTGGATCGC CACGGCTTCGTGTCCCGAGAGTTCTGTCGCACCTATGTCCTGCCTGCAGATGTGGACCCCTGGCGGGTTC GAGCTGCTCTATCCCATGATGGCATCCTTAACTTGGAGGCGCCGCGGGGTGGCCGGCATTTGGACACGGA AGTCAATGAAGTCTACATCTCCCTGCTTCCTGCTCCTCCTGACCCCGAGGAAGAGGAAGAGATAGCCAGA GTTGAGCCCTGACTGCCAGCGATAGACCCAGCACCCAGTGAATCCCCTGTCTACCTCCCGTGGTGATTCC ATAAATCCACCACACCCCAGAGGGAGCAGCATCCCTGGGAGATGGCATCGGTGCATGGTCCACAGTGTAT GGTTTGGTCCCTTGATGTATCAAAGCCTTTGATTTAATTCTGGGTGGGGCTGAATAAACCAAAACCTGGG GGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:948723 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:948723 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11464 same embryo
	EMAGE:11466 same embryo
	EMAGE:11465 same embryo
	EurExpress:euxassay_006798 same experiment
	MGI:4825480 same experiment
	EMAGE:31879 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source