EMAGE mouse gene expression spatial database

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EMAGE:11475

Galnt9 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 9 ( MGI:2677965)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006920. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006920_01	euxassay_006920_02	euxassay_006920_03	euxassay_006920_04

euxassay_006920_05	euxassay_006920_06	euxassay_006920_07	euxassay_006920_08	euxassay_006920_09

euxassay_006920_10	euxassay_006920_11	euxassay_006920_12	euxassay_006920_13	euxassay_006920_14

euxassay_006920_15	euxassay_006920_16	euxassay_006920_17	euxassay_006920_18	euxassay_006920_19

euxassay_006920_20	euxassay_006920_21	euxassay_006920_22	euxassay_006920_23	euxassay_006920_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11475_wholemount_strong.wlz

11475_wholemount_moderate.wlz

11475_wholemount_weak.wlz

11475_wholemount_possible.wlz

11475_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11475_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

interdigital region between forelimb digits 1 and 2 mesenchyme

moderate

regional

moderate expression: see section 02 03 04 05

interdigital region between forelimb digits 2 and 3 mesenchyme

moderate

regional

moderate expression: see section 02 03 04 05

interdigital region between forelimb digits 3 and 4 mesenchyme

moderate

regional

moderate expression: see section 02 03 04 05

interdigital region between forelimb digits 4 and 5 mesenchyme

moderate

regional

moderate expression: see section 02 03 04 05

interdigital region between hindlimb digits 1 and 2 mesenchyme

moderate

regional

moderate expression: see section 02 03 04 weak expression: see section 05 06

interdigital region between hindlimb digits 2 and 3 mesenchyme

moderate

regional

moderate expression: see section 02 03 04 weak expression: see section 05 06

interdigital region between hindlimb digits 3 and 4 mesenchyme

moderate

regional

moderate expression: see section 02 03 04 weak expression: see section 05 06

interdigital region between hindlimb digits 4 and 5 mesenchyme

moderate

regional

moderate expression: see section 02 03 04 weak expression: see section 05 06

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vibrissa

moderate

regional

moderate expression: see section 06 07 09 10 11 12 13 14 15 16 17 18 19 21 22 23 24 weak expression: see section 08

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 06 20 21 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 19 20

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23

vagus x ganglion

moderate

regional

moderate expression: see section 08 09 18 19

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 06 07 08 09 18 19 20

trigeminal v nerve

moderate

regional

moderate expression: see section 10 11 17 18

spinal cord

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8167
Entity Detected:	Galnt9, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 9 ( MGI:2677965)
Sequence:	sense strand is shown >T8167 GGCACGAGGTGTACCCCGAGATGAGGATCTACAACAACACCCTCACGTACGGAGAGGTGAGAAACAGCAA AGCTAGCGGCTACTGCTTGGACCAGGGGGCAGAAGACGATGACCGAGCCATCCTCTACCCCTGTCATGGC ATGTCCTCACAGCTGGTGCGCTACAGTGCCGAAGGTCTCCTGCAGCTGGGACCCCTGGGCTCTACCGCCT TTCTGCCCGACTCCAAGTGTCTGGTAGATGACGGCCGGGGACGCATGCCCACCCTGAAGAAGTGTGAGGA TGTAGCCAGGCCTACGCAGAGGCTATGGGACTTCACCCAGAGCGGCCCCATCGTGAGCCGAGACACGGGT CGGTGTCTGGAGGTAGAGATGTCTAAAGATGCCAACTTTGGGCTGCGGTTAGTAGTACAGCGCTGCTCGG GGCAGAAGTGGATGATCCGAAACTGGATCAAACACGCCAGGCACTGACCTCTGCCCTCAGCCCACCTCCA CACATTGGGGCTCTTGGACTCGTGAATGTTCTGGGACTCAGAAGGTCCTGGGGTGGAGCCAAGATGGGAA CTTCTTGCCCACCCCACAGACCCAAGAGCAATGTGGGCCCTGCACACCCCAGGGCTGGGTTTTGTCACCA GGGATCGCAGAGACCTTGGAATAGCCGCTCCAGCCTGCATTAGGAACCAAACTTTTGTGGAACAGGCAGC TCTGCCCGAGGGAGGACATCTGAGGACGGCGCACACTCATGCCAAATCACGTGCCTTTTCTACAGTGTTC CCCCAACCGGCTATGAGGATAGCCGCCACGCATGTGCTAAGTTCCTGTACATAACCCATATGGAAACAAA CAGACTCAGCTATTCAGTGCTGAGGGCAGGTGCATCCTCACACCAAGCACCCTGTCCTAGCAGGTCCGAG CCTCTCCGGTCAGCTGCCCTGGCTCGCCTGCTCAACCTCACCGTAACCTAAGGCGATCTTCAAGGGTGAC CGCTTGTGTTCTGGTAACAAGGCAATGGGAAGAGATCACCGGGATGAGACTCGGTGCACTTTTCTTCCCA AGGGTCAGCTAGGCACTCACTGGGCACGGGTCTCAGCCTGGGAAACGAGTCTCTTGTTGCTCTGTCATTT CTACAGCAAGCATCATCAAATGACCCCTCCAGCCCCTCACTTGTTTTATAAATAAAGTTTTATTGGCACA CAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5056396 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5056396 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11474 same embryo
	EMAGE:11478 same embryo
	EMAGE:11476 same embryo
	EMAGE:11477 same embryo
	EurExpress:euxassay_006920 same experiment
	MGI:4824986 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source