EMAGE mouse gene expression spatial database

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EMAGE:11523

Nmral1 NmrA-like family domain containing 1 ( MGI:1915074)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006783. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006783_01	euxassay_006783_02	euxassay_006783_03	euxassay_006783_04

euxassay_006783_05	euxassay_006783_06	euxassay_006783_07	euxassay_006783_08	euxassay_006783_09

euxassay_006783_10	euxassay_006783_11	euxassay_006783_12	euxassay_006783_13	euxassay_006783_14

euxassay_006783_15	euxassay_006783_16	euxassay_006783_17	euxassay_006783_18	euxassay_006783_19

euxassay_006783_20	euxassay_006783_21	euxassay_006783_22	euxassay_006783_23	euxassay_006783_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11523_wholemount_strong.wlz

11523_wholemount_moderate.wlz

11523_wholemount_weak.wlz

11523_wholemount_possible.wlz

11523_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11523_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

moderate

regional

moderate expression: see section 10 11 12 13 15 16 17 18 19

olfactory lobe

strong

regional

strong expression: see section 16

telencephalon ventricular layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 17 18 19 20 21 22 23 24 moderate expression: see section 01

rest of cerebellum marginal layer

strong

regional

strong expression: see section 04 05 06 07 08 18 19 20 21 22 23 moderate expression: see section 09 10 11 12 13 14 15 16 17

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 03 04 05 06 07 08 19 20 21 22 23

midbrain ventricular layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19

metanephros

strong

regional

strong expression: see section 07

renal cortex

strong

regional

strong expression: see section 08 09 10 11 12 17 18 19 20 21

left lung

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 moderate expression: see section 04

right lung

strong

regional

strong expression: see section 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8235
Entity Detected:	Nmral1, NmrA-like family domain containing 1 ( MGI:1915074)
Sequence:	sense strand is shown >T8235 GGGTATTGCCAGACAGAATCTCGGGACGCGTTCAGGGACCGGACGTCTCAGGGTAGAGATTCTGCCCTTC GCCAACTCTGCCACCCCAGGCCTCTTTGGATCACGCTCTTTGGGCTCTGGACCCCATGGCTGATAGGAAA CTGGTGGTGGTTTTTGGAGCCACAGGTGCGCAAGGTGGCTCTGTGGCCCGTGCATTGCTAGAAGATGGGA CATTCAGGATTCGAGTGGTAACAAGAAACCCTGAGCAGAGGGCAGCCAAAGAGCTGAAGCAGCAAGGTGC TGAGGTAGTGCGAGGAGACCAGGACGATGCAGCTAGCATGGAGCTGGCCTTGGCTGGAGCCCATGCCACC TTCATTGTGACCAATTACTGGGAGACGTGCAGCCAGGACCGAGAAGTGCAGCAGCCCCACCAGTGGGACC AAGTATTCAAACAAGGCAAGCTTCTAGCCGATCTAGCCAAACGCTTGGGCCTCCATTATGTAGTGTACAG TGGCCTGGAGAACATCAGGAAGCTGACGGCTGGGAAGCTGGCCGCAGGACACTTTGATGGCAAAGGGGAG GTGGAGGAATACTTCCGAGACATCGGTGTTCCCATGACCAGTGTGCGGCTGCCTTGCTATTTCGAGAATC TCCTTTCCTATTTCCTGCCCCAGAAAGCTGCAGATGGAAAAAGCTTCTTGCTGGACTTGCCCATGGGTGA CGTCCCCATGGATGGAATGTCTGTGAGTGACCTGGGCCCCGTGGTGCTCAGCTTGCTGAAGAAGCCAGAA GAGTACGTAGGGCAGAACATCGGGCTCAGTACCTGCAGGCACACCGCAGAGGAGTATGCTGCCTTGCTTA GCAAGCACACTGGCAAGGCTGTACATCATGCCAAGACAACTCCTGAGGATTACGAGAAACTTGGTTTCCA GGGGGCTCAAGACTTGGCCAACATGTTCCGTTTCTACACCCTGAAACCTGATCGGAACATTCATCTGACC CTGCGACTCAACCCCAAAGCCCAGACACTGGACCAGTGGCTGGAGCAGCACAAAGGGGACTTTGCACAGC TGTGATCTTGAAGCATTTGTAGGGACAACAAGCACAACAAACACTCTTTCTTGTATACAGTTCTGTGTTT GGGTTTTTTTTCCCTCCCAAATAAAACCATTGTTATCACAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1313824 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1313824 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11521 same embryo
	EMAGE:11526 same embryo
	EMAGE:11522 same embryo
	EMAGE:11525 same embryo
	EMAGE:11524 same embryo
	EurExpress:euxassay_006783 same experiment
	MGI:4826736 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source