EMAGE mouse gene expression spatial database

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EMAGE:11535

Rassf9 Ras association (RalGDS/AF-6) domain family (N-terminal) member 9 ( MGI:2384307)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006876. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006876_01	euxassay_006876_02	euxassay_006876_03	euxassay_006876_04

euxassay_006876_05	euxassay_006876_06	euxassay_006876_07	euxassay_006876_08	euxassay_006876_09

euxassay_006876_10	euxassay_006876_11	euxassay_006876_12	euxassay_006876_13	euxassay_006876_14

euxassay_006876_15	euxassay_006876_16	euxassay_006876_17	euxassay_006876_18	euxassay_006876_19

euxassay_006876_20	euxassay_006876_21	euxassay_006876_22	euxassay_006876_23	euxassay_006876_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11535_wholemount_strong.wlz

11535_wholemount_moderate.wlz

11535_wholemount_weak.wlz

11535_wholemount_possible.wlz

11535_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11535_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

epidermis

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 14 24 weak expression: see section 15 16 17 18 19 20 21 22 23

vibrissa

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 21 22 23 24 weak expression: see section 19

cochlear duct

strong

regional

strong expression: see section 21 22 23 24 moderate expression: see section 02 03 04 05 06 07 08 09 18 19 20

cornea epithelium

moderate

regional

moderate expression: see section 01 02 03 04

naris

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18 19

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 12 13 15 16 17 19 weak expression: see section 10 11 20 21

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 12 13 14 16 17 18

vomeronasal organ

moderate

regional

moderate expression: see section 13 16

esophagus

strong

regional

strong expression: see section 13 14

tongue epithelium

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18

lower jaw incisor

strong

regional

strong expression: see section 17 18 moderate expression: see section 12 14 19

lower jaw molar

moderate

regional

moderate expression: see section 08 20

upper jaw incisor

strong

regional

strong expression: see section 17 18 moderate expression: see section 12 14

upper jaw molar

moderate

regional

moderate expression: see section 07 21

kidney calyx

weak

regional

weak expression: see section 05 06 08 16 17 19

kidney pelvis

moderate

regional

moderate expression: see section 07 18

urethra of male

strong

regional

strong expression: see section 12 13 14

larynx

moderate

regional

moderate expression: see section 12 13 14

left lung

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12

right lung

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21 22 23 weak expression: see section 24

trachea

strong

regional

strong expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8250
Entity Detected:	Rassf9, Ras association (RalGDS/AF-6) domain family (N-terminal) member 9 ( MGI:2384307)
Sequence:	sense strand is shown >T8250 CTGAAGCTGGGCTGGTCTCCCTAGGCACAGCAGCCACAACAATCCCGCAGTTCAAAGTTAGGCAGCAATT GCACAACTTCCAGCAACTCTCTCAGCCAGCTGCTAATGAGCTTGAAAGCAAGGAACATCTGAGGAGTTGA AAAGGAAGCCTCCAGCCTTCCTCCTTTCACCAGAGAGACCCAGACACCCCTCCCAGGGCCCAGATTACTT TGAGATAAGGACTGTGTTATTTGCTTGCCTTGCCGGACAGACCATGGCTCCCTTTGGAAGAAACTTACTG AAGACACGACATAAAAACAGATCTCCAACTAAAGACATGGATCCTGAAGAGAAGGAAATTGTGGTTTGGG TTTGCCAGGACGAGAAGATTGTCTGCGGATTAACTAAACGCACCACCTCCATCGATGTTATCCAGGCTCT ACTTGAGGAACACGAAGCCACATTTGGAGAAAAGCGATTTCTGTTGGGCAAGGCCAGTGACTACTGCATC GTAGAAAAGTGGAGGGGCTCAGAGCGAGCCCTTCCTCCTCTGACCAGGATCCTTAAGCTGTGGAAGGCTT GGGGAGATGAACAGCCCAACATGCAGTTTGTTTTGGTTAAAACGGATGCCTTCCTCCCAGTTCCACTGTG GCGGACCGCTGAAACCAAACTAGTGCAAAATAATGAAAAACCTTGGGAGCTCAGCCCAGCGAATTACATG AAAACATTACCACCAGATAAACAAAAACGAATCGTCAGGAAAACCTTCCGGAAACTGGCCAAAATTAGGC AGGACACAAGTTCCCATGATCGAGACAATATGGAGTGTTTAGTTCATTTGATTATTTCTCAGGACCACAC GATTCACCAGCAAGTGCAGAGAATGAAAGAGTTAGATATGGAAATTGAAAAATGTGAAGCTAAGATCCAC TTGGACCGAGTAGGGAATGATGGGGCTAATTACGTTCACGAGGCCTATTTAATGCCTAGGTTAAGTGATG AAGAGCAGAAGTTAGACATTCAAGCTGAGATCAACCAGACTCTAGAGGACCTGAACGACAGTGAGGGGAT GGCGCAGCTGGAGGAACAGCTGCAATACTACAGAGCCCTCATCGATAAGCTCTCTGCTGAGATCGAGAGA GAGGTGAAGGGCGCAGGCATTGATGGGATTGAAGACATGGAGGGGGCAGCTGCCTGTGAGCTGGAAAACT CGGATTTGGAAAGCGTTAAGTGCGATTTGGAGACAAGTATGAAAGCTGGTTTGACAATCCACTCTCACTT GAGTGGCATCCAGAGAGAGATTAAATACAGTGACTCACTGCTTCAGATGAAAGCAAGGGAGTACGAACTC CTAGCCAAGGAGTTCAGCTCACTTCACATTAGCAACAAAGATGGATGTCAGGGAAAAGAAAACAGAGGAA AGGAAGCTGAGGCTCCCAGCAGCAATGGGGAGACCCCTCCATTAACTCAAAGGGTGTTTAACATATATAC AAATGACACGGATTCAGACACTGGCATCAGTTCAAACCACAGTCAGGACTCTGAAACGACTCTGGGAGAT GTGCTCCTGCTGGCAACTTAACTTCAATGGCTTCTTTCTGACCTTTAATGATGTTTTGGGTGGTTTATCA ACGATTCTATTCCGTATATAACATTGTCAAAATGTAAACCATGATAAAGTATTCAATAGTTAATAAAAAC TAGTGGCCACCAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1348931 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1348931 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11539 same embryo
	EMAGE:11536 same embryo
	EMAGE:11540 same embryo
	EMAGE:11538 same embryo
	EMAGE:11537 same embryo
	EurExpress:euxassay_006876 same experiment
	MGI:4827640 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source