EMAGE mouse gene expression spatial database

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EMAGE:11536

Plac9 placenta specific 9 ( MGI:2663998)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006803. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006803_01	euxassay_006803_02	euxassay_006803_03	euxassay_006803_04

euxassay_006803_05	euxassay_006803_06	euxassay_006803_07	euxassay_006803_08	euxassay_006803_09

euxassay_006803_10	euxassay_006803_11	euxassay_006803_12	euxassay_006803_13	euxassay_006803_14

euxassay_006803_15	euxassay_006803_16	euxassay_006803_17	euxassay_006803_18	euxassay_006803_19

euxassay_006803_20	euxassay_006803_21	euxassay_006803_22	euxassay_006803_23	euxassay_006803_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11536_wholemount_strong.wlz

11536_wholemount_moderate.wlz

11536_wholemount_weak.wlz

11536_wholemount_possible.wlz

11536_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11536_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 07 08 09 10 11 18 19 20

lateral ventricle choroid plexus

weak

regional

weak expression: see section 07 08 09 10 11 12 15 19

telencephalon ventricular layer

moderate

regional

moderate expression: see section 11 15 16 weak expression: see section 07 08 09 10 18 19 20

metencephalon part of 4th ventricle choroid plexus

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14 15 19

esophagus

moderate

regional

moderate expression: see section 13 weak expression: see section 12

stomach

moderate

regional

moderate expression: see section 03 06 07 weak expression: see section 01 02 04 05

bladder

strong

regional

strong expression: see section 12 13 14 15 moderate expression: see section 11

male reproductive system

weak

regional

weak expression: see section 10 14

trachea

moderate

regional

moderate expression: see section 15 weak expression: see section 14

axial skeleton

moderate

regional

moderate expression: see section 15 weak expression: see section 11 12

sternum

moderate

regional

moderate expression: see section 12 15 16 weak expression: see section 13 14

tail mesenchyme

moderate

regional

moderate expression: see section 16 weak expression: see section 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8248
Entity Detected:	Plac9, placenta specific 9 ( MGI:2663998)
Sequence:	sense strand is shown >T8248 GGCTACGTACCATGCAGGCGCTGCTCTGCGCGCTAGCCGGGCTCGCCCTGCTCCGTGCCGGGACGGGCGA ATGGGGCCAAGGTCCCAGGGACACCCCCGGACGGCGAGCTGCCGAGTCCCCCAGTTCCCCTGGAGATCTA GCTGGGAGCCCAGGCTGTGACAGACACGCGGCTGTGCAAAGGCGGTTAGACATTATGGAGGAGACGGTGG AGAAGACAGTGGAGCACCTGGAGGCGGAAGTGACAGGTCTGCTGGGCCTGCTGGAGGAACTGGCTTCAAA CCTTCCCACAGGGCCCTTCAGCCCCAAACCTGACTTGCTTGGAGATGATGGTTTCTGACTTCCAGGGATG GTGGAGCCTGCCAGCTGAAGTCATCCCTCAGAGAACCAAGCCAGGTCTTCCTGCCTTCCTGCCCCACCTT TGTGTGAAATAAAAGCTCCGATTTGGACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1348266 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1348266 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11539 same embryo
	EMAGE:11540 same embryo
	EMAGE:11538 same embryo
	EMAGE:11535 same embryo
	EMAGE:11537 same embryo
	EurExpress:euxassay_006803 same experiment
	MGI:4827249 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source