EMAGE mouse gene expression spatial database

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EMAGE:11570

Chrnb1 cholinergic receptor, nicotinic, beta polypeptide 1 (muscle) ( MGI:87890)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006741. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006741_01	euxassay_006741_02	euxassay_006741_03	euxassay_006741_04

euxassay_006741_05	euxassay_006741_06	euxassay_006741_07	euxassay_006741_08	euxassay_006741_09

euxassay_006741_10	euxassay_006741_11	euxassay_006741_12	euxassay_006741_13	euxassay_006741_14

euxassay_006741_15	euxassay_006741_16	euxassay_006741_17	euxassay_006741_18	euxassay_006741_19

euxassay_006741_20	euxassay_006741_21	euxassay_006741_22	euxassay_006741_23	euxassay_006741_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11570_wholemount_strong.wlz

11570_wholemount_moderate.wlz

11570_wholemount_weak.wlz

11570_wholemount_possible.wlz

11570_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11570_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

strong

regional

strong expression: see section 01 02 03 04 05 06 07

upper leg muscle

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 20 21 22 23 24 moderate expression: see section 03

diaphragm

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

forearm rest of mesenchyme

strong

regional

strong expression: see section 01 02 03

hand

strong

regional

strong expression: see section 01 02 03 04 05 06

tarsus rest of mesenchyme

strong

regional

strong expression: see section 07 08 09 23 24

lower leg rest of mesenchyme

strong

regional

strong expression: see section 02 04 05 06 moderate expression: see section 01 03

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

tongue muscle

strong

regional

strong expression: see section 13 14 15 16 17 18 19 20 21

tongue extrinsic muscle

strong

regional

strong expression: see section 11 12

left lung

strong

regional

strong expression: see section 07 moderate expression: see section 05 06 08 09 10 11 12 13 14

right lung

moderate

regional

moderate expression: see section 15 16 17 18 19 20 21 22 23 24

tail paraxial mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7049
Entity Detected:	Chrnb1, cholinergic receptor, nicotinic, beta polypeptide 1 (muscle) ( MGI:87890)
Sequence:	sense strand is shown >T7049 CGTCACTTCCCCTGGCCTGAGGCCGCTATCCGGACAAACTTCACTGAGCGAAAGAGGCAGGGCCATGGCC TTAGGGGCGCTGCTTCTGCTTCTAGGGGTACTCGGGACGCCCCTCGCCCCAGGCGCCCGCGGGTCGGAAG CCGAAGGCCAACTGATTAAGAAACTTTTCTCCAACTATGATAGCTCGGTGAGGCCGGCGCGGGAGGTGGG AGACCGCGTCGGGGTCAGCATCGGCCTCACCCTGGCGCAACTCATCAGCCTGAACGAGAAGGATGAAGAA ATGAGCACAAAGGTGTACTTAGACCTGGAGTGGACCGACTACAGGTTAAGCTGGGACCCCGCAGAACATG ATGGCATCGATTCTCTCCGCATCACTGCTGAATCCGTTTGGCTCCCTGATGTGGTGCTGCTGAACAACAA TGACGGAAATTTCGACGTTGCCCTGGACATCAATGTCGTGGTATCTTTCGAGGGCTCTGTGCGCTGGCAA CCCCCTGGCCTGTACCGCAGCAGCTGCAGCATCCAGGTCACCTACTTCCCCTTTGACTGGCAGAACTGCA CCATGGTGTTTAGTTCCTACAGCTATGACAGCTCTGAGGTCAGCCTGAAGACGGGCCTGGATCCTGAAGG AGAGGAGAGGCAGGAAGTCTACATTCATGAAGGGACCTTCATTGAGAATGGCCAATGGGAGATTATCCAC AAACCTTCTAGGTTAATCCAACTTCCAGGGGATCAGAGAGGTGGGAAGGAAGGGCATCATGAGGAAGTTA TCTTCTATCTCATTATTCGGCGGAAGCCTCTCTTCTACCTGGTCAACGTCATTGCCCCATGCATCCTCAT CACGCTCCTGGCCATCTTTGTCTTCTACCTCCCACCAGATGCAGGAGAGAAGATGGGGCTCTCCATCTTT GCCCTGCTGACGCTCACTGTGTTCTTGCTGCTGTTGGCCGACAAAGTGCCTGAGACCTCCCTGGCGGTGC CCATCATTATCAAGTACCTCATGTTTACCATGGTCCTTGTCACCTTCTCCGTTATCCTTAGTGTTGTGGT CCTCAACCTGCATCATCGCTCACCCCACACCCACCAAATGCCCTTTTGGGTCCGCCAGATCTTCATTCAC AAGCTCCCTCCATACCTGGGTCTGAAGAGGCCCAAACCCGAGAGAGACCAACTCCCTGAACCACATCACT CTCTTTCTCCAAGTAGTGGCTGGGGCAGAGGAACTGATGAATATTTCATCCGGAAGCCTCCAAGTGATTT TCTTTTCCCTAAACTTAACAGGTTTCAGCCTGAATCATCTGCCCCGGACCTGCGACGATTTATCGATGGT CCAACCCGGGCTGTAGGTCTGCCTCAGGAGCTACGAGAGGTCATTTCCTCAATCAGCTACATGGCCCGAC AGCTTCAGGAACAGGAGGACCACGACGCACTGAAGGAGGACTGGCAGTTTGTGGCCATGGTTGTGGACCG TCTTTTTCTGTGGACATTCATCGTTTTCACAAGCGTCGGGACCCTAGTCATTTTCCTAGATGCCACGTAC CATTTGCCCCCTCCTGAACCTTTCCCTTGAGGACCCTCCGGTCATTTTTGCCAAATGCTGCAGGTACTCT CAAGCTCAATAGCGCTTGGCGCTTGTAAGAATCTTGTTTCTGGGAAGACGTTCAAGTGAGACTTGGAGGC TGAGCATGGTGCCTTGGACCCACCCGAAATGTCCTTCTACTTATTATTTAGAGTTTTGTTCTTCTGCGGT TTTGTTTTTGTTGTTTTTGAGACAGAGTCTCACTCTGTGGCCCAGGCTAGCCTGGAACTGTGGCGTCCAG GCTGGTCTCAAACTCCCAACAATGTTACTTCCTTAGCCTAGCAATTGTTGGAATCACAGACCTGGTAAAC CAGCACACTTGGAGTTTGTTTCTTGAAACTACATAGAATGTCACAGCTTAGATCTCTAGTGAAATAGAAT TATAACCAAGTAGTAGACTGTAAGCCTTTTGAGGACAGGAGATCTGTTGGCTACTGAATTATCCCCAGCA ATGTGTACAGGTCCTAGCCCATAAGTGCTTAACATTTGTTGAAGAGAGGGAAAATAAATCTGGAAGGAAA TATAAATATCGCCTACAATTGCATGTATTAAAATTAAAATGTAGAAAATGATTAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAG
Notes:	The probe template was PCR amplified from IMAGE:4009493 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4009493 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11568 same embryo
	EMAGE:11569 same embryo
	EMAGE:11567 same embryo
	EMAGE:11566 same embryo
	EMAGE:11565 same embryo
	EurExpress:euxassay_006741 same experiment
	MGI:4823861 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source