EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11604

Gnb4 guanine nucleotide binding protein (G protein), beta 4 ( MGI:104581)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006820. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006820_01	euxassay_006820_02	euxassay_006820_03	euxassay_006820_04

euxassay_006820_05	euxassay_006820_06	euxassay_006820_07	euxassay_006820_08	euxassay_006820_09

euxassay_006820_10	euxassay_006820_11	euxassay_006820_12	euxassay_006820_13	euxassay_006820_14

euxassay_006820_15	euxassay_006820_16	euxassay_006820_17	euxassay_006820_18	euxassay_006820_19

euxassay_006820_20	euxassay_006820_21	euxassay_006820_22	euxassay_006820_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11604_wholemount_strong.wlz

11604_wholemount_moderate.wlz

11604_wholemount_weak.wlz

11604_wholemount_possible.wlz

11604_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11604_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 08 09 10 15 16

pons mantle layer

moderate

regional

moderate expression: see section 08 17

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 07 20 21 22

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 08 18 19

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22 23

vagus x ganglion

strong

regional

strong expression: see section 09 17

trigeminal v nerve

strong

regional

strong expression: see section 10 17

ventral grey horn

moderate

regional

moderate expression: see section 10 11 12 13 14 15

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 10 11 16

cervical ganglion

moderate

regional

moderate expression: see section 10 16

thoracic ganglion

moderate

regional

moderate expression: see section 12

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 11 12 16 17

neural retina

moderate

regional

moderate expression: see section 01 02 03 04 23

heart valve

moderate

regional

moderate expression: see section 08

aortic valve

strong

regional

strong expression: see section 13

mitral valve

strong

regional

strong expression: see section 14

pulmonary valve

strong

regional

strong expression: see section 12

tricuspid valve

strong

regional

strong expression: see section 11 13

clavicle

strong

regional

strong expression: see section 11 16 moderate expression: see section 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8249
Entity Detected:	Gnb4, guanine nucleotide binding protein (G protein), beta 4 ( MGI:104581)
Sequence:	sense strand is shown >T8249 CCAGCTAGCGGTTCGCGACCCGCAGCCGGGCGCCCAGCAGTAGCGCGGTGGTGGCGGCCGGGCTCCAGCT CTTCACTTGAGACGCCTGAGGGAAACCACCAGGCAGGATGAGCGAGCTGGAGCAGCTGAGGCAGGAGGCT GAACAGCTTCGGAATCAGATCCAGGATGCTCGGAAGGCCTGCAACGATGCCACGCTGGTTCAGATCACGT CTAATATGGACTCCGTGGGCCGAATACAAATGCGAACAAGGCGCACGCTGCGTGGCCACCTCGCTAAGAT CTACGCCATGCACTGGGGATATGATTCCAGGCTACTAGTCAGTGCTTCGCAAGATGGAAAATTAATTATT TGGGATAGCTATACGACAAATAAGATGCACGCCATCCCTCTGAGGTCCTCCTGGGTGATGACCTGTGCCT ACGCCCCGTCCGGGAACTACGTTGCCTGTGGAGGCTTGGATAACATCTGCTCCATATACAACCTAAAGAC CCGAGAGGGGAATGTGCGGGTGAGCCGAGAATTGCCAGGACACACGGGCTACTTGTCCTGCTGCCGATTC TTAGATGATGGACAAATCATTACAAGTTCGGGAGACACGACTTGTGCTTTGTGGGACATTGAGACCGGAC AGCAGACTACGACCTTCACAGGACACTCGGGTGACGTGATGAGCCTCTCACTGAGTCCTGACTTGAAGAC CTTTGTGTCTGGTGCTTGTGATGCATCCTCAAAGCTGTGGGATATCCGAGATGGGATGTGTAGACAGTCT TTCACCGGACACATCTCAGACATCAACGCTGTCAGCTTCTTCCCGAGTGGATATGCCTTTGCCACTGGTT CTGATGATGCCACATGCCGACTCTTTGACCTCCGTGCAGACCAGGAGCTCCTGCTATACTCTCATGACAA TATCATCTGTGGCATTACTTCTGTGGCCTTCTCAAAGAGTGGGCGCCTCCTGTTAGCCGGCTATGACGAC TTCAACTGCAGTGTGTGGGACGCTCTGAAAGGAGGCCGGTCAGGTGTCCTTGCTGGTCATGACAACCGTG TTAGCTGCTTAGGTGTGACTGATGACGGCATGGCTGTGGCCACTGGCTCCTGGGACAGTTTTCTTAGAAT CTGGAATTGAGTGCCATATTTTCTGTTCTCCAATGATACCTGGAGAAATCCGTGTTACAGCCTATAGCTG TGAGGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1348753 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1348753 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11605 same embryo
	EMAGE:11607 same embryo
	EMAGE:11603 same embryo
	EMAGE:11602 same embryo
	EMAGE:11606 same embryo
	EurExpress:euxassay_006820 same experiment
	MGI:4825133 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source