EMAGE mouse gene expression spatial database

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EMAGE:11607

Edaradd EDAR (ectodysplasin-A receptor)-associated death domain ( MGI:1931001)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006770. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006770_01	euxassay_006770_02	euxassay_006770_03	euxassay_006770_04

euxassay_006770_05	euxassay_006770_06	euxassay_006770_07	euxassay_006770_08	euxassay_006770_09

euxassay_006770_10	euxassay_006770_11	euxassay_006770_12	euxassay_006770_13	euxassay_006770_14

euxassay_006770_15	euxassay_006770_16	euxassay_006770_17	euxassay_006770_18	euxassay_006770_19

euxassay_006770_20	euxassay_006770_21	euxassay_006770_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11607_wholemount_strong.wlz

11607_wholemount_moderate.wlz

11607_wholemount_weak.wlz

11607_wholemount_possible.wlz

11607_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11607_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 12 13 14 15 16

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 10 11 17 18 19 20

pancreas

moderate

regional

moderate expression: see section 09 10 11 12 16 17

vibrissa

strong

regional

strong expression: see section 20 moderate expression: see section 06 07 08 09 21

cornea

moderate

regional

moderate expression: see section 01 02 03 04 05 22

anterior naris

strong

regional

strong expression: see section 13 14 16 17

external naris

strong

regional

strong expression: see section 13 18

pharynx epithelium

strong

regional

strong expression: see section 12 13 14 15 16

tongue epithelium

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17

stomach

moderate

regional

moderate expression: see section 04 05 06 07 08

lower jaw incisor

strong

regional

strong expression: see section 13 16 17 moderate expression: see section 12

lower jaw molar

strong

regional

strong expression: see section 08 19 moderate expression: see section 07

upper jaw incisor

strong

regional

strong expression: see section 16 moderate expression: see section 12 13 17

upper jaw molar

strong

regional

strong expression: see section 08 20 moderate expression: see section 07

urethra of male

moderate

regional

moderate expression: see section 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8244
Entity Detected:	Edaradd, EDAR (ectodysplasin-A receptor)-associated death domain ( MGI:1931001)
Sequence:	sense strand is shown >T8244 CTGCGATCCTGCTGTTCTCAGGGGTCCTAGAGACTGTCCACCCCTGCTCGGACACTCATCTGTCCTCTCC TGGTCTTCATGGCTTCTCCGGACGACCCTCTGCGCTCAGATCACATGGCAAAGGAGCCAGTGGAGGACAC GGACCCCAGCACTTTATCCTTTGCCATGTCAGACAAGTATCCCATTCAAGACACCGGCCTCCCTAAAGCT AAAGAGTGTGATACAGTCAATTCCAACTGTCCACCAAATTCTGATGACCAACCTCAGGGAGAAGAAAATG ACTTTCCAGACAGCACCAAAGATCCCCTTTCAGGTGTCAGCAGAAACCAGCCCTGTAAGGACGGGAAGGG CAGCTGCTCTTGCCCTTCCTGCTCCCCCCGGGCCCCCACCATCAGCGACTTGCTCAACGATCAGGACTTG CTAGATACGATCAGGATAAAGCTGGATCCATGTCACCCAACCGTGAAGAACTGGAGGAATTTTGCCAGCA AATGGGGCATGCCCTATGATGAACTGTGCTTCCTGGAACAGAGGCCCCAGAGCCCCACGCTGGAGTTCTT ATTCCGAAACAGCCAGAGGACTGTGGGCCAACTGATGGAGCTCTGCCGGCTGTACCACAGGGCCGATGTG GAGAAGATCCTGCGCAGGTGGGTGGATGAGGAGTGGCCCCACCGGGGACACTCCGACAGCTCCATGCACT TCTAGAATCCCCTCTCCTCTGGCATTGGCCCTACTGCTCGTTGGACCAGCATGGCTCCTGCGGAGAGCGT ACCTGCTCCTAAGGTTTTCAGGATAAGGTGTGGGCAGTGGTCGTCCCCGAAGCTGGCAGCTTTTGGAAAG ACACGTTGTTTGGATGGCAGGTATCCGTTTCCTTTTGCACATTTGTTTTAATTTAATATTTGAGCCTGGG ATTGGGGAAGAATATTTGGTAGGTCACACAGGGCCAAAGCCTGTGGACAGAGACATGCCAACATGGAAAG AAAATGACACCCTCTTTAAATACTGCCAAAGATTGCAGCGGGATCCCAAAATATCAATGTCTTTGGACAT GTCCGCATACAGAATCCCATACAGAATCTCTGCAGTCCTGGCCAGAGGATGGCAGGGAGAAGTCATCCCG GCCGGTTCACTGTCACTGTTTCTGTTGAAAAAGGCTCAGGAGAGCAAAAGGATACTACTGTATAATGTGG CCCAGGTATATGGGCTCTTTTTTTTTTAATCAAATACTTTTGTAATCTATAGGCTATCAATACTGTCATT GAATTTAGCATTTTTAAAAGAAATACAAAATATATGGTTTGTAGGAGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1344937 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1344937 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11605 same embryo
	EMAGE:11603 same embryo
	EMAGE:11604 same embryo
	EMAGE:11602 same embryo
	EMAGE:11606 same embryo
	EurExpress:euxassay_006770 same experiment
	MGI:4824470 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source