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EMAGE:11614

A330049M08Rik RIKEN cDNA A330049M08 gene ( MGI:2444888)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11614 EMAGE:11614 EMAGE:11614 EMAGE:11614 EMAGE:11614
"Pseudo-wholemount" of euxassay_003653. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003653_01 euxassay_003653_02 euxassay_003653_03 euxassay_003653_04
EMAGE:11614 EMAGE:11614 EMAGE:11614 EMAGE:11614 EMAGE:11614
euxassay_003653_05 euxassay_003653_06 euxassay_003653_07 euxassay_003653_08 euxassay_003653_09
EMAGE:11614 EMAGE:11614 EMAGE:11614 EMAGE:11614 EMAGE:11614
euxassay_003653_10 euxassay_003653_11 euxassay_003653_12 euxassay_003653_13 euxassay_003653_14
EMAGE:11614 EMAGE:11614 EMAGE:11614 EMAGE:11614 EMAGE:11614
euxassay_003653_15 euxassay_003653_16 euxassay_003653_17 euxassay_003653_18 euxassay_003653_19
EMAGE:11614 EMAGE:11614 EMAGE:11614 EMAGE:11614 EMAGE:11614
euxassay_003653_20 euxassay_003653_21 euxassay_003653_22 euxassay_003653_23 euxassay_003653_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11614Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11614_wholemount_strong.wlz
11614_wholemount_moderate.wlz
11614_wholemount_weak.wlz
11614_wholemount_possible.wlz
11614_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11614_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 21 22 23 24
humerus
moderate moderate
regionalmoderate expression: see section 03 04 05 20 21 22 23
hindlimb digit 1 metatarsal
strong strong
regionalstrong expression: see section 03 23
hindlimb digit 1 phalanx
strong strong
regionalstrong expression: see section 03 22
hindlimb digit 2 metatarsal
strong strong
regionalstrong expression: see section 02 03 23
hindlimb digit 2 phalanx
strong strong
regionalstrong expression: see section 03 21 22 23
hindlimb digit 3 metatarsal
strong strong
regionalstrong expression: see section 02 03 23
hindlimb digit 3 phalanx
strong strong
regionalstrong expression: see section 03 21 22 23
hindlimb digit 4 metatarsal
strong strong
regionalstrong expression: see section 03 23
hindlimb digit 4 phalanx
strong strong
regionalstrong expression: see section 03 21 22 23
hindlimb digit 5 metatarsal
strong strong
regionalstrong expression: see section 04
hindlimb digit 5 phalanx
strong strong
regionalstrong expression: see section 22 23
hip mesenchyme
strong strong
regionalstrong expression: see section 01 02 10 18 19 24
knee mesenchyme
strong strong
regionalstrong expression: see section 01 02 10 18 19 24
lower leg mesenchyme
strong strong
regionalstrong expression: see section 01 02 10 18 19 24
upper leg mesenchyme
strong strong
regionalstrong expression: see section 01 02 10 18 19 24
femur
strong strong
regionalstrong expression: see section 07 08 09 11 20 21 weak expression: see section 04
pectoral girdle and thoracic body wall musculature
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 16 17 18 19 20 21 22 23 24
otic capsule
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 18 19 20 21 22 23
hyoid
strong strong
regionalstrong expression: see section 09 10 weak expression: see section 11
stomach
strong strong
regionalstrong expression: see section 08 10 moderate expression: see section 05 06 07
hindgut
weak weak
regionalweak expression: see section 14 15 16
rectum
weak weak
regionalweak expression: see section 14
midgut
weak weak
regionalweak expression: see section 07 08 09 10 11 12 13 14 15 16 17
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 11 13 14 15
lower jaw molar
moderate moderate
regionalmoderate expression: see section 10 11 12 15 16 weak expression: see section 08 09
bladder
moderate moderate
regionalmoderate expression: see section 13 14 15
urethra of male
weak weak
regionalweak expression: see section 13 14
trachea
strong strong
regionalstrong expression: see section 11 12 13
axial skeleton
strong strong
regionalstrong expression: see section 08 10 11 12 13 14 15 16 moderate expression: see section 07 09 17
basisphenoid bone
strong strong
regionalstrong expression: see section 06 07 08 09 15 16 17 23 24
exoccipital bone
strong strong
regionalstrong expression: see section 01 22 23 24
temporal bone
strong strong
regionalstrong expression: see section 23 24
orbito-sphenoid
strong strong
regionalstrong expression: see section 01 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
viscerocranium
strong strong
regionalExpression in the turbinate bone.
scapula
moderate moderate
regionalmoderate expression: see section 03 04 21 22
axial skeleton tail region
strong strong
regionalstrong expression: see section 12 13 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6603
Entity Detected:A330049M08Rik, RIKEN cDNA A330049M08 gene ( MGI:2444888)
Sequence:sense strand is shown

>T6603
GGGGTGTGGAGCAGGTAGGGCGCAGGGCACGGAACGCGGGGGCTTGGAAGGACAGCGCACAGGGGCTCAG
GCCCGCGGAAGATAGCTCTCCGGACCCCCAGGGGTCCCCAGCCTGGCGCGCGCCTTGGCGTGAGCCGGTA
GCTGAAAGGTCAGCAACATCTCCCGGTTGTCTGCAGACACCGGTGCACAGAGGTGTTCAGGGTGCAAGCT
GAGAAGCACCCCGAAGCTGGGTGCATTCGGGGCTACATCTCTGGCATGCGGAAAGCTGGTGGTTTCCTTG
TCCGCTCCTACGAAGTCACCGGGAGCGACCTGGACTCCCGGAAGAAAAGCCTACGAGAGGAACAGAGATG
CCATTGAGAGCTGTCGCTGCCGGCATGAGGCAAGATCGGCTCTGGGGTTGCTCACTGTTCTTCTTGAAGG
AGGAGTGACTGGCCGCCACCGGCACCTGGAACCCAGCACCCAGGAGGTGAACTCGGACGGACCTGAGGAG
GATCCTGTGTCCTGTGTCCTTGGGAGACTACTCACTGGTGCATCTCAGGGCAGAGGAGTGGCAGAACACG
GGCATTCCACGGGGCTGCTCTTCAGCTTGCAGACTAACTGAGCACAAGAGTGCGCTTTGTAGCTCTGAGC
CTCAGGTCCCGATGGGAAGCCATGGGAAAGGAAACCCTGTTTTCTGTGGCATCTTGTACTGGGAGCAGGG
GCGAGATGACCACAGCCACCACTTTGGGGGACGCCGTCTTCTCGCTGAACATGACCAGGGGAGAGGACGC
CCTGTATAAGAGCTCTGGAGCCATCGTGGCTGCCATCGTGGTGGTTGTCATCATCATTGTCACCTTGGTG
CTGATCCTGCTGAAGATGTACAACAGGAGAATGAGGACCAGGCGGGAGCTGGAGCCCAAGAGCCCAAAGC
CACCTGTCCCTCCTGCCCTGGACCCCAGCAGCAATGGCAGCCAGCAGCCTGCTACTGTGACCTTCGACCC
TGCCAATGTCCATGTGGAGACTCGGTGACCCTGCCTTGGCGCCATTCCTGCCTCTCTGCAAAGAGCTTTG
ATGACCTGAAAGTCCACTCTGCTGGCAGCTTCACCAAGTTCTTTCTGGTTGGGTAAACTGAGGCACCCTT
CAAACAGCCCCTGCAGCTTGAAGCCACCCTCACCTGTGCTCTGGCCACCCCAGCTATGTCCACGTCCCTG
CTGCCACTCGACCAAAGCTCTGAGGACATTTCTTCTGTTACCTGACGAGAAGCACTGAGCTGGGGAAACT
GGTGTGAGTCTGGTTGTCGCCTCACAAGTGCGACGGCTAGACAGCTTGATAGACAGACATCTGAGTCTTC
TGCTCATGGTCAGGGAGTGTTAAAGAGAGTGTTCCCCAGGGAGCCACTGGGAGCATCTCAACTTAAGGGC
ATGTCCACAGCTTCCTCAATCCCATAGCCAGATGCAGGGAAACTAACTTGGACTAACTAGCCACAAAACC
AACTTGGTAGCATCTAACTGATGCAGTACTAGATGGCCACTAGCCAGCTCCTAATGGCCAGTCAGCGCTC
TCCCCCTTTAACAAAGGGTACAGAGGCTGGATAAAGTGGCACGTGCCTGTCATCACAGTGCTCAGTAAAA
GCGGGAACACTGGGATTGCAGGACCAGCTTTGACCACATTCAGAGATGCTTCTCTTTAAAAAAAAAAAAA
AA
Notes:The probe template was PCR amplified from IMAGE:3595770 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3595770 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11617 same embryo
 EMAGE:11616 same embryo
 EMAGE:11613 same embryo
 EMAGE:11615 same embryo
 EMAGE:11612 same embryo
 EurExpress:euxassay_003653 same experiment
 MGI:4822829 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS