EMAGE mouse gene expression spatial database

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EMAGE:11627

Anxa9 annexin A9 ( MGI:1923711)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006531. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006531_01	euxassay_006531_02	euxassay_006531_03	euxassay_006531_04

euxassay_006531_05	euxassay_006531_06	euxassay_006531_07	euxassay_006531_08	euxassay_006531_09

euxassay_006531_10	euxassay_006531_11	euxassay_006531_12	euxassay_006531_13	euxassay_006531_14

euxassay_006531_15	euxassay_006531_16	euxassay_006531_17	euxassay_006531_18	euxassay_006531_19

euxassay_006531_20	euxassay_006531_21	euxassay_006531_22	euxassay_006531_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11627_wholemount_strong.wlz

11627_wholemount_moderate.wlz

11627_wholemount_weak.wlz

11627_wholemount_possible.wlz

11627_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11627_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

moderate

spotted

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

vibrissa

strong

regional

strong expression: see section 05 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 17 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 06 07

trigeminal v nerve

moderate

regional

moderate expression: see section 09 17

dorsal root ganglion

moderate

regional

moderate expression: see section 05 09 14 15 17 weak expression: see section 06 07 08 10 11 12 16

heart ventricle

strong

spotted

strong expression: see section 10 13 moderate expression: see section 06 07 08 09 11 12 14 15 16 17 18

bladder

strong

regional

strong expression: see section 13 14 15

kidney calyx

strong

regional

strong expression: see section 09 moderate expression: see section 07 08 16 17 18 19

kidney pelvis

moderate

regional

moderate expression: see section 08 18

lung

strong

spotted

strong expression: see section 03 04 05 06 07 08 10 11 12 13 14 15 16 17 18 19 20 21 22 23 moderate expression: see section 09

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7958
Entity Detected:	Anxa9, annexin A9 ( MGI:1923711)
Sequence:	sense strand is shown >T7958 CCTCACCCCAGCTGCCTGAAGAGCCAGGCCAGGGAAGGGAGGAGGAACTTGAGCCTCAGAGGGCATCACC TCAGACTAAGGGTTCAGGGGACCTGGAGTGGGGGGAACTGGTTGGGAAGCCAGCTGCACCATGTCTGCGA GCTGTGGACCACTGGGAACATCTCTGACTCAGGAGATCCTCAGCTCCCTGGGTCTTGCCGACAAGACCGC AGCCTGGGGGACCCTGGGCACCCTCAGGACGTTCTTGAGTTTCAGTGTGGACAAGGATGTACAAAGACTG CTGAAGGCTATCGCAGGCCAAGGTGTGGACTATGATACCATTGTGGATGTACTGACCAATCGGAGCAGAG AGCAAAGACAGCTCATTTCTCGAGCCTTTCAAGAGCGCACCAAACAGGATCTATTGAAGTCCTTGCAGGC AGCGCTCTCTGGCAATCTGGAGAAAATCGTGGTGGCTCTCCTACAGCCTGCAGCCCAATTTGATGCCCAG GAACTGAGGACAGCCTTGAAGACCTCTGGTTCTGCTGAGGATGTAGCCTTGGAAATCCTGGCCACCCGAG CAGCGCCCGGACTGCAGGCATGCCTGGCAGTATATAAGCATGACTTCCAGGTGGAGGCTGAGGAAGATAT CAGGACTGAGACCAATGGTATCCTGCAGGACCTGCTTCTGGCCCTGAGCAAGGGGGACCGTGAGAGCTAT TCTGGAATTATTGATTACAACTTGGAAGAACAAGATGTCCGGGCACTTCAGCAGGCTGGAGAATCCAGTA CAGCAGGGCAATGGGTCCTGCTCCTTACACAACGCAGTCCTGAACACCTCATCCGAGTGTTTGATCAGTA CCGGAGATGCACTGGCCAGGAACTGGAGGATGCTATCCGGAACTGTTTCCATGGAGATGCCCAGCTGGCT CTGATCAGCCTAGCTTCCATGTTGAGAAACACAGCGCTGTACTTTGCTAACAAACTCCACCAAGCCCTTC AGGAAACTGAGCCTAATTTCCAAGTGCTGACGCGGGTCCTTATCTCCCGAAGCGAGAGTGACCTTCTAAG CATTCGGGCTGAGTTCAAGAAGAAATTTGGGAAGTCTCTCTATTCTTCTCTTCAGGATGTAGTCAGAGGG GATTGTCGTTCAGCACTACTGGCCCTGTGTAGAGCTGAAGATATCTGAGACCTTCCTGACCCACCCTGAC GCGACACTCGAAACCGCTGAGATTCCCATATTTGGTTGAAAACATGGAAGAACCCCAAGGAACACCATGC ACCCTGGCCTCCTCTCGAGGCTGGACCTCTGAGTTCCTTTGGATTTCCCTCTTCTCAGACTGGCATCTTC CAGCTCCATTCTAGAGAGGAGTAACGGGAGTTCTCTGACAGTCTCTGCCCTGCTCAATCCCTCCTAAGGC CAGAACATCTCCCTGGGAGTCAAATGTTTTGGCAGTTCACCATCTTTTTGTTTCCTGGTTAAAGTCTGGG TGGAGGAGGCCATGGATCAGGAGGAGGCCATGGAGTTGAGGTGCCTTTGAAGTATGCTCAGCCATTCAAC AGGAATCCTGTTTTTACCCCAGTACTGCACTTAACAGTTACCAACACACCCCACTCCTGGATCTTCTCCC CAGGAAATCTCACGTAATTAAGAACAGTAAAAAGAAAGAGCGGAAGAGCCAACATTTGGGTTCCCCCTGC TGGCTCTGTGAGAAATGACAGGCAACCTGTCGCCTCCCTGGCTCCTCCCCTGTTCACATTCCTCAACCGG GTCAAATATTTTACTCCCCTGCTCCAAGGTAGGAACGCAAATCTCAGGAAAGAAGTTTTATTTCCAAACC TCTAGGAAGGCATTAAAAATAATAAAGATAGAAACTCAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30290956 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30290956 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11628 same embryo
	EMAGE:11630 same embryo
	EMAGE:11629 same embryo
	EMAGE:11631 same embryo
	EMAGE:11632 same embryo
	EurExpress:euxassay_006531 same experiment
	MGI:4823156 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source