EMAGE mouse gene expression spatial database

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EMAGE:11629

Pole2 polymerase (DNA directed), epsilon 2 (p59 subunit) ( MGI:1197514)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006546. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006546_01	euxassay_006546_02	euxassay_006546_03	euxassay_006546_04

euxassay_006546_05	euxassay_006546_06	euxassay_006546_07	euxassay_006546_08	euxassay_006546_09

euxassay_006546_10	euxassay_006546_11	euxassay_006546_12	euxassay_006546_14	euxassay_006546_15

euxassay_006546_16	euxassay_006546_17	euxassay_006546_18	euxassay_006546_19	euxassay_006546_20

euxassay_006546_21	euxassay_006546_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11629_wholemount_strong.wlz

11629_wholemount_moderate.wlz

11629_wholemount_weak.wlz

11629_wholemount_possible.wlz

11629_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11629_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

shoulder mesenchyme

strong

regional

strong expression: see section 02 03 04 20 21 22

upper arm mesenchyme

strong

regional

strong expression: see section 02 03 04 20 21 22

hand mesenchyme

strong

regional

strong expression: see section 01 02 03 04 22

foot mesenchyme

strong

regional

strong expression: see section 05 19 20 21 22

lower leg mesenchyme

strong

regional

strong expression: see section 01 02 03

upper leg mesenchyme

strong

regional

strong expression: see section 02 03 04 05 19 20 21 22

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

diencephalon roof plate

strong

regional

strong expression: see section 13

choroid invagination

strong

regional

strong expression: see section 05 06 07 08 09 10 11 15 16 17 18

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

tongue extrinsic muscle

strong

regional

strong expression: see section 09 10 11 12 13 14 15

tongue intrinsic muscle

strong

regional

strong expression: see section 10 11 12 13 14 15 16

tail paraxial mesenchyme

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7959
Entity Detected:	Pole2, polymerase (DNA directed), epsilon 2 (p59 subunit) ( MGI:1197514)
Sequence:	sense strand is shown >T7959 CGCCAAATCGCGAACATGGCGCCGGAGCGGCTGCGGAGCCGCACGGTCTCAGCTTTCAAGCTGCGGGGCT TGCTGCTCAGGAGTGAAGCCACCAAGTATCTGACTGAAGCTCTCCAGTCTGTCACTGAATTGGAGCTAGA GGAGAACCTAGGAAAGATCATCGATGCAGTTGAAAAGCAGCCCTTGTCCTCGAACATGATCGAACGATCT GTCGTGGAGGCAGCTGTGCAAGAGTGCAGTCAGTCTGTGGATGAGACTATAGAGCACGTTTTCAATATCA TTGGAGCTTTTGATATTCCACGTTTTATATATAATTCAGAAAGGAAAAAATTTCTTCCTCTATCCATGAC TAATCATGCTGCACCAACCTTATTTGGAACAGCAAGAGACAAAGCCGAGCTGTATCTTGAACGCTATACC ATTTTACACCAGAGGACACACAGACATGAATTGTTCACTCCTCCGGTGATAGGCTCACACCTTGAAGAAA GTGGGAGCAAGTTCCAGCTTAAAACTATAGAAACCTTACTGGGTAGCACAAGCAAAGTTGGTGACGTGAT CGTCCTGGGGATGATAACCCAGCTGAAAGAGGGGAAGTTTTTCCTGGAAGATCCTACTGGAACAGTACAA CTGGACCTTAGTAAAGCTCAGTTCCACAGTGGCTTATACACCGAGGCCTGCTTCGTCCTGGCAGAAGGTT GGTTTGAAGATCAAGTGTTTCACGCCAATGCCTTTGGATTTCCTCCCACTGAGCCCTCCAGCACCACAAG GGCGTACTATGGAAATATTAATTTTTTTGGAGGGCCTTCTAATGCATCAGTGAAGACGTATACAAAGCTG AGACAGCTGGAAGATGAGAACAAGGACGCCATGTTTGTGATTGTGTCTGATGTTTGGTTGGACCAAGTGC AAGTGTTGGAGAAGTTTCATGTAATGTTTTCTGGTTACTCGCCAGCACCTCCAACCTGCTTTATTCTGTG TGGTAATTTTTCATCTGCACCATATGGAAAAAATCAAGTCCAGGCTTTGAAAGATTCCCTAAAATCTTTG GCAGACATAATATGTGAATATCCAAGTATCCACCAAAGTAGCCGTTTTGTGTTTGTGCCTGGCCCAGAGG ATCCTGGATTTGGTTCCATTTTACCAAGGCCGCCACTCGCTGAAAGCATCACACAGGAATTCAGACAAAG GGTTCCGTTTTCAGTTTTCACGACTAATCCTTGCAGAATTCAGTATTGTACACAAGAAATAATTATCTTC CGTGAAGACTTAGTGAATAAAATGTGTAGAAACTGTGTCCGTTTTCCTAGCAGCAGTTTGGATATTCCTA CTCACTTCGTAAAGACCATCTTATCTCAGGGACATCTGGCTCCTCTCCCTCTTTACGTATGCCCCGTCCA CTGGGCATATGATTACACGTTGAGAGTGTACCCGGTGCCCGACCTGCTGGTCATTGCAGACAAGTACGAC CCGTTCACCGTGACCAACACCGATTGCCTCTGCATAAACCCTGGCTCCTTTCCAAGAAGTGGATTTGCAT TCAAAGTTTTTTACCCTTCCAGTAAAACAGTAGAAGACAGCAAACTTCAGGGCTTTTGAGATCGATGAGA ATATATGAAATGAATTTTCTTATTTTTATCTTATGTATTTTGTATATTAAATTGTAATAAACTTATGATA AACCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30147626 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30147626 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11628 same embryo
	EMAGE:11630 same embryo
	EMAGE:11627 same embryo
	EMAGE:11631 same embryo
	EMAGE:11632 same embryo
	EurExpress:euxassay_006546 same experiment
	MGI:4827316 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source