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EMAGE:11632

Art1 ADP-ribosyltransferase 1 ( MGI:107511)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11632 EMAGE:11632 EMAGE:11632 EMAGE:11632 EMAGE:11632
"Pseudo-wholemount" of euxassay_006561. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006561_01 euxassay_006561_02 euxassay_006561_03 euxassay_006561_04
EMAGE:11632 EMAGE:11632 EMAGE:11632 EMAGE:11632 EMAGE:11632
euxassay_006561_05 euxassay_006561_06 euxassay_006561_07 euxassay_006561_08 euxassay_006561_09
EMAGE:11632 EMAGE:11632 EMAGE:11632 EMAGE:11632 EMAGE:11632
euxassay_006561_10 euxassay_006561_11 euxassay_006561_12 euxassay_006561_13 euxassay_006561_14
EMAGE:11632 EMAGE:11632 EMAGE:11632
euxassay_006561_15 euxassay_006561_16 euxassay_006561_17

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11632Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11632_wholemount_strong.wlz
11632_wholemount_moderate.wlz
11632_wholemount_weak.wlz
11632_wholemount_possible.wlz
11632_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11632_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
upper arm muscle
strong strong
regionalstrong expression: see section 01 02 03 15 16 17
upper leg muscle
strong strong
regionalstrong expression: see section 02 03 04 05 06 15 16 17
diaphragm
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17
forearm rest of mesenchyme
strong strong
regionalstrong expression: see section 01
hand
strong strong
regionalstrong expression: see section 02 03
foot
strong strong
regionalstrong expression: see section 04 05 17
lower leg rest of mesenchyme
strong strong
regionalstrong expression: see section 02 03 04
vertebral axis musculature
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17
adenohypophysis
strong strong
regionalstrong expression: see section 07 08 09 10 11
pharyngo-tympanic tube
strong strong
regionalstrong expression: see section 16 17 moderate expression: see section 01 15
eye skeletal muscle
strong strong
regionalstrong expression: see section 02 03 04 15 16 17
eyelid
strong strong
regionalstrong expression: see section 01 02 03 17
anterior naris
strong strong
regionalstrong expression: see section 10 12
heart ventricle
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 12
esophagus epithelium
strong strong
regionalstrong expression: see section 07 08 09
tongue muscle
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11
stomach proventricular region
strong strong
regionalstrong expression: see section 03 04 05 06
bladder
strong strong
regionalstrong expression: see section 11
urethra of male
strong strong
regionalstrong expression: see section 10 11
left lung
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09
right lung
strong strong
regionalstrong expression: see section 10 11 12 13 14 15 16 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7962
Entity Detected:Art1, ADP-ribosyltransferase 1 ( MGI:107511)
Sequence:sense strand is shown

>T7962
GTCAGCTTTGCCCGCCATGGAGAAGGCTAGATGAGGAAAAAGAGACAGCAGCTTGCCCGAGGTCACCTCC
ACCATGAAGATTCCTGCTATGATGTCTCTCCTACTGGTGTCTGTGGGTCTCAGGGATGGAGTTCAGGTTC
AAAGTTACTCCATCTCACAACTAGACATCTTTTCTCAAGAAACACCCCTGGACATGGCCCCAGCGTCCTT
TGATGACCAGTATGCTGGCTGCCTGGCAGACATGACAGCGGCCCTGCCAGATCTCAACCACTCAGAGTTT
CAGGCCAACAAAGTATACGCGGATGGCTGGGCTCAGGCCAACAATCAGTGGCAGGAGCGCAGGGCCTGGG
GGTCCGTGTGGGGCTCCTTGCCCCCATCACCGCCGGGCTTCCGGGATGAGCACGGGGTGGCGCTGCTGGC
CTACACCGCCAACAGCCCCCTGCACAAGGAGTTCAACGCAGCTGTGCGTGAGGCGGGACGTTCTCGGGCC
CATTACCTCCACCACTTCTCCTTCAAGACCCTCCACTTCCTGCTCACCGAGGCCCTGCAACTGCTCCGGA
GCCACCGATCTCGCGGGTGCCAGCAGGTGTACAGGGGGGTGCACGGCCTGCGCTTTCGGCCAGCGGGGCC
TGGCGCCACCGTTAGGCTGGGGGGCTTTGCTTCTGCGTCCCTCAAGAACGTTGCCGCCCAACAATTTGGC
GAGGACACCTTCTTCGGTATCTGGACCTGCCTTGGGGCCCCCATCAGGGGCTACTCCTTTTTCCCTGAAG
AGGAGGAGGTGCTGATCCCTCCTTTCGAAACTTTCCAGGTGATCAATACAAGCCGACCCACCCAGGGTCC
CGCACGCATCTACCTCCGCGCTCTGGGCAAACGCAGTACATACAACTGTGAATACATCAAAGAAAAGAAG
TGCAGGTCTGGGCCCTGCTGGCTGGGTAGCTCAGCCCCAGGCTCCATTTCTGCTTCCTGCTCTCTTCTGC
TGCTGCTCTTGTTCCTTGTGCTGAGCGCCCTTCCAGAGAATCCAGGTCTCCAGCAACTTACCCGATGTTG
ACCGGCCCTGACTGAAGAAGGCTGGTCAACTGTGCGCCTTCAGTGTAGCAGGATCCTGGTGAATCAGTCC
ATCTACAGGGAACTCTGGGACCGTCTATGGCAATTGCCAGACACATCGATAGAGACAGAGAAGAGAATTT
GGAGACTGAACCTCATCCAGGGATGTAGGGACGGGACTGAGGACAAGCAGAGGACTCGAGGACAGGCAGA
GACTCAACTTGGAGCCAGGAGACTTCAACTCATTGTGCGTGACACACACACATGACCGTGGGCCAAGCTG
GAGTCACTTAATTGCCTCTCATAAAGGTATGAGTGGTAGTACCAGGCTAATAAATGTGATTTTTAAAGAG
ATGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:30137479 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30137479 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11628 same embryo
 EMAGE:11630 same embryo
 EMAGE:11627 same embryo
 EMAGE:11629 same embryo
 EMAGE:11631 same embryo
 EurExpress:euxassay_006561 same experiment
 MGI:4823262 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS