EMAGE mouse gene expression spatial database

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EMAGE:11642

Them4 thioesterase superfamily member 4 ( MGI:1923028)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008480. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008480_01	euxassay_008480_02	euxassay_008480_03	euxassay_008480_04

euxassay_008480_05	euxassay_008480_06	euxassay_008480_07	euxassay_008480_08	euxassay_008480_09

euxassay_008480_10	euxassay_008480_11	euxassay_008480_12	euxassay_008480_13	euxassay_008480_14

euxassay_008480_15	euxassay_008480_16	euxassay_008480_17	euxassay_008480_18	euxassay_008480_19

euxassay_008480_20	euxassay_008480_21	euxassay_008480_22	euxassay_008480_23	euxassay_008480_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11642_wholemount_strong.wlz

11642_wholemount_moderate.wlz

11642_wholemount_weak.wlz

11642_wholemount_possible.wlz

11642_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11642_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 10 11 18 19

pons mantle layer

weak

regional

weak expression: see section 09 19

facial vii ganglion

weak

regional

weak expression: see section 06 07 08 09 22 23

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 09 21 22

trigeminal v ganglion

weak

regional

weak expression: see section 05 06 07 08 09 10 11 19 20 21 22 23

vagus x ganglion

weak

regional

weak expression: see section 10

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 07 09 21 22

trigeminal v nerve

weak

regional

weak expression: see section 11 19

cervico-thoracic ganglion

weak

regional

weak expression: see section 13 19

cervical ganglion

weak

regional

weak expression: see section 11 13 19

thoracic ganglion

weak

regional

weak expression: see section 14 15 17 18

dorsal root ganglion

weak

regional

weak expression: see section 09 10 11 12 13 14 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35642
Entity Detected:	Them4, thioesterase superfamily member 4 ( MGI:1923028)
Sequence:	sense strand is shown >T35642 TAGTGTGGCTCTTTCTGGAACAGAGCAGAGAGCAGCGTCCTGCCCTCGGCTTGAGCAATGCTGAGAAACT GCGCTATGCGCCTGCGCACTCTTGGGGCCACGCCTGCGAGGCGGCCAGGAGCAGCTAGGAGGTTATTTTC TTCTGAGAAAGTCATTCGTAAGGACTATGCTCTCCCTAACCCCAGCTGGACTAAGGACCTCAGACTCCTT TTTGATCAGTTCATGAAGAAATGTGAAGATGGCTCCTGGAAACGCATGCCTTCACACAGACAAAACCCTA CTCGAGCTATTCAAGAGTTCCAAACCCTTTTTGTTGACTCGAAGTTTAAGAAAGAAGAACAAATGTCAAA GGCCCAACAGTTCACCAGAAGTTTCGAGGAAGGGCTAGGCTTTGAGTATGCGATGTTCTATAATAAAGTT GAGAAGAGGACGGTCTCCTTGTTTCAAGGAGGACTTCACCTGCAAGGAGTACCTGGATTTGTTCACGGAG GTGCCATTGCAACCATCATTGATGTCACTACTGGCACGTGTGCAATTTCCGAAGGCGTTGCCATGACTGC CAACCTCAACATCACCTATAA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 77722. Forward Primer - name:077722_F_cDNA_4921507I02Rik, sequence:TAGTGTGGCTCTTTCTGGAACA; Reverse Primer - name:077722_N_SP6_cDNA_4921507I02Rik, sequence:TTATAGGTGATGTTGAGGTTGGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11644 same embryo
	EMAGE:11640 same embryo
	EMAGE:11643 same embryo
	EMAGE:11639 same embryo
	EMAGE:11641 same embryo
	EurExpress:euxassay_008480 same experiment
	MGI:4828697 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source