EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11655

Rnf138 ring finger protein 138 ( MGI:1929211)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008413. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008413_01	euxassay_008413_02	euxassay_008413_03	euxassay_008413_04

euxassay_008413_05	euxassay_008413_06	euxassay_008413_07	euxassay_008413_08	euxassay_008413_09

euxassay_008413_10	euxassay_008413_11	euxassay_008413_12	euxassay_008413_13	euxassay_008413_14

euxassay_008413_15	euxassay_008413_16	euxassay_008413_17	euxassay_008413_18	euxassay_008413_19

euxassay_008413_20	euxassay_008413_21	euxassay_008413_22	euxassay_008413_23	euxassay_008413_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11655_wholemount_strong.wlz

11655_wholemount_moderate.wlz

11655_wholemount_weak.wlz

11655_wholemount_possible.wlz

11655_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11655_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

strong

regional

strong expression: see section 09 moderate expression: see section 07 08 10 17 18 19 20

pancreas

moderate

regional

moderate expression: see section 10 11 12 13 16 17 18

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vibrissa

moderate

regional

moderate expression: see section 07 08 09 10 21 22 23

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 17 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 08 17 18 19

trigeminal v nerve

moderate

regional

moderate expression: see section 10

spinal cord

moderate

regional

moderate expression: see section 08 09 10 11 12 14 15 16 17

peripheral nervous system

moderate

regional

moderate expression: see section 13

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 03 04 05 24

retina

moderate

regional

moderate expression: see section 01 02 03 04 05 24

naris

moderate

regional

moderate expression: see section 13 14 17

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 12 13 17 18 19 20 weak expression: see section 10 11

lower jaw incisor

moderate

regional

moderate expression: see section 12 13 16 17 18

lower jaw molar

moderate

regional

moderate expression: see section 08 19 20

upper jaw incisor

moderate

regional

moderate expression: see section 12 13 17 18

upper jaw molar

moderate

regional

moderate expression: see section 08 19 20

metanephros

moderate

regional

moderate expression: see section 07 08 09 10 11 12 17 18 19 20 21 22

seminiferous cord

strong

regional

strong expression: see section 06 07 08 21 moderate expression: see section 22 23

left lung

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12

right lung

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6578
Entity Detected:	Rnf138, ring finger protein 138 ( MGI:1929211)
Sequence:	sense strand is shown >T6578 CACCACCACTACTACCACCACCACCTCGGCTCCTGCCGGCGCCGTCGCCTCTCCCGCCCACCCCTCGCCA TGTCCGAGGAACTTTCGGCGGCCACGTCCTACACGGAAGATGATTTCTACTGCCCTGTCTGTCAGGAGGT GCTCAAGACGCCGGTGCGGACCGCGGCCTGTCAGCACGTTTTCTGTAGAAAATGTTTCCTGACTGCAATG AGAGAAAGTGGAATACATTGTCCCCTATGTCGTGGAAGTGTGACTAGAAGAGAAAGAGCATGTCCGGAAC GGGCCTTAGATCTTGAAAATATCATGAGGAGGTTTTCTGGTAGCTGCAGATGCTGTTCAAAAAAGATTAA ATTCTATCGCATGAGACATCATTACAAATCTTGTAAGAAGTATCAGGATGAATATGGTGTTTCTTCTGTC ATTCCAAACTTTAAGATTTCTCAAGATTCAGTAAGGAGCAGTAATAGGAGTGAAACATCTGCATCTGATA ACACAGAAACTTATCAAGAGGATACAAGTTCTTCTGGGCATCCTACCTTTAAGTGTCCCTTATGTCAAGA GTCAAATTTCACCAGACAACGTTTATTGGATCACTGTAATAGTAACCACCTATTTCAGATAGTTCCTGTG AATCTTCAGCTAGATGAGGAAACCCAATATCAAACTGCTGTGGAAGAGTCTTTTCAAGTAAACATGTGAC ATGTATAGACATCTCTGCCTCCTTGCAACCTACAAGTGCCATCTTTAAGGAGAAGACATGAAGTCACCAT TTTCAGTAATTTGCTGTGCATATTAATAAAAATAATAATTCAGTCTACTGTATTAGGTTTTTAATTGAAA ATAAAGGTGGGCCACCCTAATACCATTCTCTAGACAGTTACTTTAACAGCATGGAAAGGGTTGTATTTCA CTTGTGTGGTGAAAAGAGAATCTCTGTTGTCTTTTTCTTCCTTGTATTACATATTCTCAATGTTTCATTA AGTTGTTTTTGGTATTTGATATAGTTCCTTCTGTTTAGTACAGAGATAACAGCAAATTCTGAACGATGTG ATTCTTAAAAAGCTAATAAACCTGAGCCATTTGTCAGAGCTGTAGAATGGAAACTTGAAGTGTGAAGTGG GATAATCCAAAGGGATTTTTTTTTAAAGTATAGATTCTAGCTGAGGAATTCAACAATAAGAAAGTTGTAT TTATGTAATGTTTAGTATTTTTGAAGACTAGTGAGATTTCTTTAATAATTTTTACTTTGAAAGCATATTG TACAAATGTTTCTTCTTTTGCTATTAGAAGAACATCAAGAGAAGTTTCCTTTGGTGGTTAGTTTGTTATT TCAATCTAGGTTGAATAATTTGTAAGCCTAAATGTTATATACCACAGTTCTTTGTAGTCAGTATTTCTCA CTGGGTGATGAAACTTTTCAGCCAGTGAATGATACATTCAATTAGTTTTTTAAAAATCCAAAGTTGCAGA TGTATGTGGATATGTACATAGACTTTTGCATGTATATATACACATATATATCTTTGCCTAGAGTTTGTCA GGTTATGTATAGAATTTCTATTAAAAAGTTTTAATAATGGACAAGCAATATAGGATTGAAGTATTTATCT CCTTTGTTTAAAATTTTGTATGTTACCAAGTTTTTAAAACAGTAAGCCAAATACTATGTGGTACAGTTGG CTGTTATTACACCTGAAAAATGTTAAATGGTGCTCACTTGTTACGTTTGAAAATGATGCATAACTGACGT GTGGTGAGAGATTTTACCAGCTACTGTTTCACTACATTTTAGTCAAAACAAAGTTTGTTCTTAATCTTTG GTATAAAGTGTTGTAGAGAAGGCCAAGTCACAAAGTAAAGGGTGAAGGGGGAATTCTGACATTCCACACT AACATAACACTGTTATGCTTTCTTTAAAATAACTAACCGCAAAAGAAAATCTCTGAAGTAGTTTGCTGCT AATATATACATATATTGTAAAAAAAAAAGGTATATTTTGATTTTCTTAATACCCTTGTTGACTTTTCTAC AATGAACATTTTTAAACTTGATTTAATAAAAATGTTAATTTTGGATGTGCAGTTTTGTAAAAAACCTTTT TCAGTTAAACGGTAATGACTTTATTTATGTTCAGTAATATAGACTCACAAGAAGCCATACATCTTAATGC CTTTTTTTCTGTTGTTTCTTGAGCGTGATGTTTACTGTGTTCTCTAGCACTTTGAGTTGACATACAAGAG TAATTCTAGGAACATCTATATGATGAACCCTTTAAAAGGAGACTTCTGTCCACAGTCCTCTAGTTTAAAG CAATAACTTGGATACTTTTAAAGTTTTATTTAGCCAGTGTGCTCCTAAATCATCCTGGGATGTACAAACT TAAAGGGAAACTTGTACTAGAGAGGGTTCCTGTTTTGAAGTCTTGTGGATGAGTTATATTAATAGATTAA TAGCTTTTTCATGAGAATTATCTTTTTTTTTGCATTAGTAACTTCTTAAATATAGAAAAAACTTTTTAAT TCTTCTTTTGTCAGTGGATTAAGAATAAGGATTGACTTTAGAATTTAATTTATGGGGGCGCACCTCAGTA GTCATGTTTAATAGCTATCAGGATAAAGGAGAGAAACTATAGCAATATGCTGATACATCAGTTTCACAGT ACCAGTTAGAAAAGCCACTTCTCCTTCAACTTAGTTTAGTTTATGTCCATAAACACAGTGTTGATTGAGA TGTATTGCTTTACTTTTCAATTAAAATGGTTTTCTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3593899 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3593899 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11660 same embryo
	EMAGE:11657 same embryo
	EMAGE:11658 same embryo
	EMAGE:11659 same embryo
	EMAGE:11656 same embryo
	EurExpress:euxassay_008413 same experiment
	MGI:4827762 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source