Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11712

4930544O15Rik RIKEN cDNA 4930544O15 gene ( MGI:1922423)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11712 EMAGE:11712 EMAGE:11712 EMAGE:11712 EMAGE:11712
"Pseudo-wholemount" of euxassay_011201. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011201_01 euxassay_011201_02 euxassay_011201_03 euxassay_011201_04
EMAGE:11712 EMAGE:11712 EMAGE:11712 EMAGE:11712 EMAGE:11712
euxassay_011201_05 euxassay_011201_06 euxassay_011201_07 euxassay_011201_08 euxassay_011201_09
EMAGE:11712 EMAGE:11712 EMAGE:11712 EMAGE:11712 EMAGE:11712
euxassay_011201_10 euxassay_011201_11 euxassay_011201_12 euxassay_011201_13 euxassay_011201_14
EMAGE:11712 EMAGE:11712 EMAGE:11712 EMAGE:11712 EMAGE:11712
euxassay_011201_15 euxassay_011201_16 euxassay_011201_17 euxassay_011201_18 euxassay_011201_19
EMAGE:11712 EMAGE:11712 EMAGE:11712 EMAGE:11712 EMAGE:11712
euxassay_011201_20 euxassay_011201_21 euxassay_011201_22 euxassay_011201_23 euxassay_011201_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11712Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11712_wholemount_strong.wlz
11712_wholemount_moderate.wlz
11712_wholemount_weak.wlz
11712_wholemount_possible.wlz
11712_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11712_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
lung
moderate moderate
spottedmoderate expression: see section 08 11 16 18 19 20 21 22 weak expression: see section 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37377
Entity Detected:4930544O15Rik, RIKEN cDNA 4930544O15 gene ( MGI:1922423)
Sequence:sense strand is shown

>T37377
CAGCCCACTGTTGTACTGGATAAACAAGCGGCGTTACCATGGCATGAATGTAGCCATTAACACAGGCCCT
CCCCCTGCTGTCACCAAGACCGAGCCGGAAGATCAGAATTCAGATTCTTTGTGGGAGTTGGATCTCTCTG
AAGGCAGGAACTTCGTTGTACAAGACAGCAGCCCCAGAGGTGAAGCCTCTGATCTCTTGCAACATGTTTT
GGGGATTCCAAAGCAGCCCCAGTCTTCAAAAATGTCACAGCCTCGGACTGACTCCCCATTCCCACTTCCC
ATCTTTCAGGAGGTGCCCTTTGCCCTGTCCCTCTGCCACCTGCCTCCCATGTTGAACCACTCTGTCTCCT
ACCCTTTGGCCAACCGCCCTGAAAGGAATGTTCCTTTCTGTTCCCTCCCAACACTGGCCCACGGAACAAA
CTGCTTTAATGCCAAGCCTTTTGCTTTAGAGCTGTAGTTTCCTCTTAGTGAGGGTGGGAGCTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 78112. Forward Primer - name:078112_F_cDNA_4930544O15Rik, sequence:CAGCCCACTGTTGTACTGGATA; Reverse Primer - name:078112_N_SP6_cDNA_4930544O15Rik, sequence:CAGCTCCCACCCTCACTAAGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11709 same embryo
 EMAGE:11713 same embryo
 EMAGE:11711 same embryo
 EMAGE:11710 same embryo
 EurExpress:euxassay_011201 same experiment
 MGI:4822736 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS