EMAGE mouse gene expression spatial database

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EMAGE:11731

Pbk PDZ binding kinase ( MGI:1289156)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006544. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006544_01	euxassay_006544_02	euxassay_006544_03	euxassay_006544_04

euxassay_006544_05	euxassay_006544_06	euxassay_006544_07	euxassay_006544_08	euxassay_006544_09

euxassay_006544_10	euxassay_006544_11	euxassay_006544_12	euxassay_006544_13	euxassay_006544_14

euxassay_006544_15	euxassay_006544_16	euxassay_006544_17	euxassay_006544_18	euxassay_006544_19

euxassay_006544_20	euxassay_006544_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11731_wholemount_strong.wlz

11731_wholemount_moderate.wlz

11731_wholemount_weak.wlz

11731_wholemount_possible.wlz

11731_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11731_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 weak expression: see section 05 06 07 08 17 18 19 20 21

adrenal gland

moderate

regional

moderate expression: see section 06 07 08 16 17

thymus primordium

strong

regional

strong expression: see section 09 10 12 13 moderate expression: see section 11

sublingual gland primordium

strong

regional

strong expression: see section 06 07 08 09 14 15 16 17

pancreas

moderate

regional

moderate expression: see section 05 06 07 08 weak expression: see section 09

thyroid gland

strong

regional

strong expression: see section 09 13 14

vibrissa

moderate

regional

moderate expression: see section 05 06 08 20 21

hypothalamus ventricular layer

strong

homogeneous

strong expression: see section 09 10 11 12 13

diencephalon lateral wall ventricular layer

strong

homogeneous

strong expression: see section 09 10 11 12 13

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 10 11 15 16

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 05 06 13 15 moderate expression: see section 07 08 09 10 11 12

rest of cerebellum marginal layer

strong

regional

strong expression: see section 02 03 04 05 06 13 14 16 17 18 19 moderate expression: see section 07 08 11 12

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 02 03 04 05 06 09 10 13 14 16 17 18 19 moderate expression: see section 07 08 11 12

midbrain ventricular layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15

ventral grey horn

moderate

single cell

moderate expression: see section 08 09 10 11 12

spinal cord ventricular layer

moderate

regional

moderate expression: see section 09 10 11

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 08 14

cervical ganglion

moderate

regional

moderate expression: see section 07 15

thoracic ganglion

moderate

regional

moderate expression: see section 11 12 13

neural retina

moderate

regional

moderate expression: see section 01 02 03

naris

moderate

regional

moderate expression: see section 12 16

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18 weak expression: see section 08 09 10 11

vomeronasal organ

moderate

regional

moderate expression: see section 14

mandible

moderate

regional

moderate expression: see section 21 weak expression: see section 03 04 05 06 07 08 16 17 18 20

lower jaw incisor

strong

regional

strong expression: see section 10 11 14 15

lower jaw molar

moderate

regional

moderate expression: see section 05 07 weak expression: see section 19

maxilla

weak

regional

weak expression: see section 05 06 07 08 16 17 18

upper jaw incisor

strong

regional

strong expression: see section 10 11 15 16

upper jaw molar

moderate

regional

moderate expression: see section 05 06 07 weak expression: see section 19

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

metanephros

moderate

regional

moderate expression: see section 06 07 08 09 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6754
Entity Detected:	Pbk, PDZ binding kinase ( MGI:1289156)
Sequence:	sense strand is shown >T6754 GCGGGAGGGAGCTGTTCCTGCATTTTCTGGAGCGAGTCTTCTGACTGCTTTTAGTTAGAACTCCAGTGCC CCTCGGCGGGCCGCGGCCTTTGAAAATGCGCGCGCCCTAAACGCTGCGGCGGTTACGCTGTTGGCGGGAG GGAGCTGAGCCTGCACTTTCCGGACTAGGTGTCCAGACAGCTTTGAGCCAGCCCGTCACTTTCACCTTTT TACCCGAGCGTGCGAGCGTGGACCTAACGTGATTGCTACAATGGAAGGAATTAATAATTTCAAGACGCCA AACAAATCTGAAAAAAGGAAATCTGTATTATGTTCCACTCCATGTGTAAATATCCCTGCCTCTCCATTTA TGCAGAAGCTTGGCTTTGGGACTGGGGTCAGCGTTTACCTAATGAAAAGATCTCCAAGAGGGTTGTCTCA TTCTCCTTGGGCCGTGAAAAAGATAAGTCTTTTATGCGATGATCATTATCGAACTGTGTATCAGAAGAGA CTAACTGATGAAGCTAAGATTTTAAAAAACCTTAATCACCCAAACATTATAGGATATCGTGCTTTTACTG AAGCCAGTGATGGTAGTCTGTGCCTTGCTATGGAGTATGGAGGTGAAAAGTCTCTGAATGACTTAATAGA AGAGCGGAACAAAGACAGTGGAAGTCCTTTTCCAGCAGCTGTAATTCTCAGAGTTGCTTTGCACATGGCC AGAGGGCTAAAGTACCTGCACCAAGAAAAGAAGCTGCTTCATGGAGACATAAAGTCTTCAAATGTTGTAA TTAAAGGTGATTTTGAAACAATTAAAATCTGTGATGTAGGAGTCTCTCTGCCATTGGATGAAAATATGAC TGTGACTGATCCTGAGGCCTGTTATATTGGTACTGAGCCATGGAAACCCAAGGAAGCGTTGGAAGAAAAT GGCATCATTACTGACAAGGCAGATGTGTTTGCTTTTGGCCTTACTCTGTGGGAAATGATGACTTTATGTA TTCCACACGTCAATCTTCCAGATGATGATGTTGATGAAGATGCAACCTTTGATGAGAGTGACTTCGATGA TGAAGCATATTATGCAGCTCTGGGGACAAGGCCATCCATCAACATGGAAGAGCTGGATGACTCCTACCAG AAGGCCATTGAACTCTTCTGTGTGTGCACTAATGAGGATCCTAAAGATCGCCCGTCTGCTGCACACATCG TTGAAGCTTTGGAACTAGATGGCCAATGTTGTGGTCTAAGCTCAAAGCATTAACTTGTATGGGAACTGTT AACTAGATATATGTAGTTAATATAACTTATGGTAGCTAGATTCTAGAAGTAGCTTTAACACTAGTGACCC CTGTCTAAGATGACTTAAGAATCAAGGGACCATTGCTTTGTTACAGATCTTTTTAGATATTCTTGCTTCT TTAGTGGGTTACTAAAAATTTCACTACGTACATGTGGTACAGATATCTGTCTGCTCATAGTGTCAGTCCT TCAGCTGGCCTGTCAGCCCATGCGCCCTGGGACTTGAGAAGAGTTCATAAACGTAGCTCCTAGGGTGTCT TGCCTCTCTACACTTAGCTTTTAATTTATTACTTTGTTTCTACTGATTGTGTCTTAAGTCTTTTAAAATA AATGTAAGAATAAACAATAAAAGACAGTTTTAGTACCAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3673060 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3673060 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11730 same embryo
	EMAGE:11732 same embryo
	EMAGE:11729 same embryo
	EurExpress:euxassay_006544 same experiment
	MGI:4827069 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source