EMAGE mouse gene expression spatial database

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EMAGE:11801

Clca2 chloride channel calcium activated 2 ( MGI:1931471)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007074. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007074_01	euxassay_007074_02	euxassay_007074_03	euxassay_007074_04

euxassay_007074_05	euxassay_007074_06	euxassay_007074_07	euxassay_007074_08	euxassay_007074_09

euxassay_007074_10	euxassay_007074_11	euxassay_007074_12	euxassay_007074_13	euxassay_007074_14

euxassay_007074_15	euxassay_007074_16	euxassay_007074_17	euxassay_007074_18	euxassay_007074_19

euxassay_007074_20	euxassay_007074_21	euxassay_007074_22	euxassay_007074_23	euxassay_007074_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11801_wholemount_strong.wlz

11801_wholemount_moderate.wlz

11801_wholemount_weak.wlz

11801_wholemount_possible.wlz

11801_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11801_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

moderate

regional

moderate expression: see section 09 10 11 weak expression: see section 18 19 20 21

thymus primordium

moderate

regional

moderate expression: see section 12 13 15

pancreas

strong

regional

strong expression: see section 10 11 moderate expression: see section 12

thyroid gland

strong

regional

strong expression: see section 11 16

hypothalamus mantle layer

moderate

regional

moderate expression: see section 10 15 weak expression: see section 08 09 16 17

thalamus mantle layer

weak

regional

weak expression: see section 07 08 17 18

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 09 10 15 16 17

pons mantle layer

moderate

regional

moderate expression: see section 07 17 18

viscerocranium

moderate

regional

Expression in the turbinate bone.

aorta

strong

regional

strong expression: see section 11 12 13 14 15

heart atrium

moderate

regional

moderate expression: see section 07 08 09 10 14 15 16 17 18 19 20 21 22 23

heart ventricle

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

midgut

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 16 17 18

liver lobe

strong

single cell

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

bladder

strong

regional

strong expression: see section 12 13 14 15

metanephros

strong

regional

strong expression: see section 09 10 11 18 19 20 21

ovary

strong

regional

strong expression: see section 05 06 07 08 09 21 22 23

urethra of male

strong

regional

strong expression: see section 13 14 15

trachea

weak

regional

weak expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6411
Entity Detected:	Clca2, chloride channel calcium activated 2 ( MGI:1931471)
Sequence:	sense strand is shown >T6411 CGGACGCGTGGGTGCGACCATGGTGCCAGGGCTGCAGGTCCTTCTGTTCCTCACCCTGCATCTCCTGCAG AACACAGAGAGCTCCATGGTGCATCTCAACAGCAATGGATACGAGGGTGTGGTCATTGCCATTAACCCCA GTGTGCCAGAGGACGAAAGGCTCATCCCAAGCATAAAGGAAATGGTAACTCAAGCTTCTACCTACCTGTT TGAAGCCAGCCAAGGAAGAGTTTATTTCAGGAACATAAGCATATTAGTCCCGATGACCTGGAAGTCAAAA TCTGAGTACTTAATGCCAAAACGAGAATCGTACGACAAAGCAGACGTCATAGTTGCGGATCCTCACCTGC AACATGGAGACGACCCCTACACCCTTCAGTATGGACAGTGTGGGGACAGAGGACAGTACATACACTTCAC TCCAAACTTCCTACTCACTGATAACTTGCGTATCTATGGACCCCGAGGCAGAGTCTTTGTCCATGAGTGG GCCCATCTCCGGTGGGGAGTATTTGATGAGTATAACGTGGACCGGCCTTTCTACATTTCTAGAAAGAACA CTATAGAAGCAACAAGGTGCTCCGCCAGCATCACAGGCAAGAAGGTGGTCCACGAGTGTCAGAGAGGCAG CTGTGTGACAAGGGCGTGCCGGCGTGACTCGAAGACACGGCTGTATGAACCCAAATGTACATTTATCCCA GACAAAATACAGACAGCTGGGGCCTCCATAATGTTCATGCAAAACCTCAATTCTGTGGTTGAATTTTGCA CAGAAAATAACCACAATGCAGAAGCCCCAAACCTACAAAACAAAATGTGCAATCGCAGAAGCACGTGGGA TGTAATCAAGGCGTCTGCTGACTTTCAGAATTCCCCTCCCATGAGAGGAACAGAAGCGCCTCCTCCACCT ACATTTTCATTGCTCAAGTCCAGAAGGCGAGTGGTGTGCTTGGTGCTGGATAAATCTGGAAGCATGGACA AAGAAGACCGTCTTATTCGAATGAATCAAGCAGCAGAACTGTACTTAACTCAAATTGTGGAAAAGGAGTC TATGGTTGGATTAGTCACATTTGACAGCGCTGCCCACATCCAAAATTATCTAATAAAAATAACGAGTAGT AGTGACTACCAAAAGATCACCGCAAACCTCCCCCAACAGGCTACTGGTGGAACTTCAATTTGCCATGGAC TCCAGGCAGGATTTCAGGCAATTACCTCCAGTGACCAGAGCACTTCCGGTTCTGAGATCGTATTGCTGAC AGATGGGGAAGATAATGGAATAAGTTCCTGCTTTGAGGCCGTCTCTCGCAGCGGTGCCATCATCCACACC ATCGCTCTGGGGCCTTCTGCTGCCCGAGAACTGGAGACTCTGTCAGACATGACAGGAGGGCTTCGTTTCT ATGCCAACAAACATGTAAGCAGCCTTATCGACGCTTTCAGTAGGATTTCATCTACAAGTGGCAGCGTCTC CCAGCAGGCTCTGCAGTTGGAGAGCAAAGCCTTCAATGTCAGAGCAGGGGCATGGATAAACAGTACAGTA CCTGTGGACAGTACCGTCGGCAACGACACGTTCTTTGTTATCACCTGGACGGTACAAAAGCCAGAAATCA TTCTTCAAGATCCAAAAGGAAAAAAATATATAACCTCAGATTTCCAAGATGATGAACTAAACATCCGGTC TGCTCGACTTCAAATACCGGGCACTGCAGAGACAGGTACTTGGACTTACAGCATCACGGGTACCAAGTCT CAGTTGATTACAATGACAGTGACCACTCGAGCAAGAAGTCCCACCATGGAACCACTCCTGGCTACCGCTC ACATGAGTCAGAGCACAGCCCAGTACCCTAGCCGGATGATTGTGTATGTACGGGTCAGCCAAGGGTTTTT GCCTGTTCTAGGAGCCAATGTCACAGCCATCATAGAAGCTGAACATGGACATCAAGTCACCCTGGAGCTA TGGGACAATGGGGCAGGTGCTGATACTGTTAAAAATGATGGCATCTACACAAGATACTTTACAGATTATC ATGGAAATGGTAGATACAGCCTAAAAGTGCGTGTCCAGGCAAGAAAAAACAAAGCCAGACTAAGCTTAAG ACAGAAGAACAAGTCTTTATATATACCTGGCTATGTGGAAAATGGTAAAATTGTACTGAATCCACCCAGA CCAGATGTCCAAGAAGAAGCCATAGAAGCTACAGTGGAGGACTTCAACAGAGTAACCTCTGGAGGGTCAT TTACTGTGTCTGGAGCGCCCCCTGATGGCGACCATGCTCGTGTGTTCCCACCAAGTAAAGTCACAGACCT GGAGGCTGAGTTTATAGGTGATTATATTCAACTTACATGGACGGCCCCTGGCAAGGTTCTCGATAAGGGA AGAGCGCATAGATACATCATCAGAGTGAGCCAGCATCCTCTGGGTCTCCAAGAAGATTTTAACAATGCTA CTTTAGTGAATGCTTCCAGTCTGATACCTAAAGAGGCTGGCTCAAAAGAAACATTTAAATTCAAACCAGA AACTTTTAAAATAGCAAATGACACCCAGCTCTACATTGCAATCCAGGCATACAATGAAGCTGGTCTCACC TCTGAGGTCTCCAACATTGCACAGGCTGTCAAGTTTACTTCTCTAGAAGATAGTATCTCTGCACTGGGTG CTGATATTTCTGCAATCTCTATGACAGTTTGGGGGTTAGCTGTGATTTTTAACTCTATTTTAAACTAGAA GATACAATAGCACTAAAATGCAACCCTGTATATATTTGCTGACTGTTGCTTTAGAATGACTTTACTACAC TCAAAGGCTGCCTGTCAACACTTGTAATATAGAAGTTCATATTCAAAGTTGAAAATCCCGAGTTACTAAC AACATTCTTCTGCTATATGTAGATCAAGATTAACAGTTCCTCATTCAATTTCCTAATTGTTCCATTTACT ATGGAAATTAGATATCCATTCCCTTTTCACAGTGTGATGCAGGTTCACTTTACAAAATATTACCAAAATA AAATTTGTATAAGTCAATTAAAGGGAATTATTCTTATATAATAGCAAACAATTTTATAGTAGAATTATTT GTAAGACAATTAATAAAGAATGCTACCTGCTTTTACAACTGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3583804 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3583804 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11803 same embryo
	EMAGE:11802 same embryo
	EMAGE:11804 same embryo
	EMAGE:11799 same embryo
	EMAGE:11800 same embryo
	EurExpress:euxassay_007074 same experiment
	MGI:4823893 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source