EMAGE mouse gene expression spatial database

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EMAGE:11834

Trim2 tripartite motif-containing 2 ( MGI:1933163)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008433. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008433_01	euxassay_008433_02	euxassay_008433_03	euxassay_008433_04

euxassay_008433_05	euxassay_008433_06	euxassay_008433_07	euxassay_008433_08	euxassay_008433_09

euxassay_008433_10	euxassay_008433_11	euxassay_008433_12	euxassay_008433_13	euxassay_008433_14

euxassay_008433_15	euxassay_008433_16	euxassay_008433_17	euxassay_008433_18	euxassay_008433_19

euxassay_008433_20	euxassay_008433_21	euxassay_008433_22	euxassay_008433_23	euxassay_008433_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11834_wholemount_strong.wlz

11834_wholemount_moderate.wlz

11834_wholemount_weak.wlz

11834_wholemount_possible.wlz

11834_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11834_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

strong

regional

strong expression: see section 01 02 03 04 moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

sublingual gland primordium

strong

regional

strong expression: see section 12 13 18 19

submandibular gland primordium

strong

regional

strong expression: see section 09 10 11 19 20

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

strong

regional

strong expression: see section 05 06 07 08 21 22 23

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 19 20

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 10 19 20 21 22 23 24

vagus x ganglion

strong

regional

strong expression: see section 09 19

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 08 09 19 20 21

trigeminal v nerve

strong

regional

strong expression: see section 11 19

spinal cord

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18

cervico-thoracic ganglion

strong

regional

strong expression: see section 11 12 16 17 18

cervical ganglion

strong

regional

strong expression: see section 10 18 moderate expression: see section 19

thoracic ganglion

strong

regional

strong expression: see section 13 14 15 16

lens

strong

regional

strong expression: see section 01 02 03

neural retina

strong

regional

strong expression: see section 02 03 04 05

anterior naris

strong

regional

strong expression: see section 14 15 18

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 10 11 12 13 14 17 18 19 20 21 22

naso-lacrimal duct

strong

regional

strong expression: see section 10 11 20 21 22

vomeronasal organ

strong

regional

strong expression: see section 14 17

esophagus epithelium

strong

regional

strong expression: see section 14 15

stomach

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11

stomach mesentery

strong

spotted

strong expression: see section 03 04 05 06 07 08 09 10 11

hindgut

strong

regional

strong expression: see section 16

midgut

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

duodenum caudal part mesentery

strong

spotted

strong expression: see section 13 14 15 16 17 18 19 20 21 22

jejunum mesentery

strong

spotted

strong expression: see section 13 14 15 16 17 18 19 20 21 22

rest of midgut mesentery

strong

spotted

strong expression: see section 13 14 15 16 17 18 19 20 21 22 23

bladder

strong

regional

strong expression: see section 15 16 17

metanephros

strong

regional

strong expression: see section 07 08 09 10 11 12 18 19 20 21 22 23

larynx

strong

regional

strong expression: see section 13 14 15

left lung

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12

right lung

strong

regional

strong expression: see section 13 14 15 16 17 18 19 20 21 22 23 24

trachea

strong

regional

strong expression: see section 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9146
Entity Detected:	Trim2, tripartite motif-containing 2 ( MGI:1933163)
Sequence:	sense strand is shown >T9146 GGCAGGTCATTTAAATCCTCAGCTTTGGTTTCCTTTTCTCTAAGACAGACAGTGCTTTCCTTCACTAGTC TGATGTCATAGGATGCGGTCGGGCTCCTGAGAGATGACCTGCCCTTCATTTGATCCATGAACAGCTTTCT CTAGTCTTTGGGGAACACTCCATGGAAAATCTAGCCAACAGTGATGGCCGATTAGCTATTCCTTCCTACA CTGCCTCACCATCCAGGGAGTCCTGACCGAAGCATCCTAACAGCAGCGTGCAGGGTCGAGGACAGCCGGC CCCCCATGTCAGTGGTCTAGGATGGCCAGTGAGGGCGCCAGCATCCCAAGTCCCGTGGTGCGCCAGATTG ACAAGCAGTTTCTGATCTGCAGTATATGCCTGGAACGGTACAAGAACCCCAAGGTCCTGCCCTGTCTGCA CACTTTCTGCGAGAGGTGCCTGCAGAACTACATCCCAGCCCACAGTTTAACCCTCTCCTGCCCGGTGTGT CGCCAGACATCCATCCTGCCCGAGAAAGGGGTGGCCGCACTTCAGAACAACTTCTTTATCACGAACCTCA TGGATGTGCTGCAGCGGACGCCAGGCAGCAACGGCGAGGACTCTTCCATCCTGGAGACGGTCACCGCTGT GGCTGCAGGAAAGCCTCTTTCGTGCCCAAACCATGATGGAAATGTGATGGAATTTTACTGCCAGTCCTGT GAGACCGCCATGTGTCGGGAGTGCACGGAGGGGGAGCACGCGGAGCACCCCACAGTCCCGCTGAAGGATG TGGTGGAGCAGCACAAGGCCTCGCTCCAGGTCCAGCTGGACGCAGTCAACAAAAGACTCCCAGAAATAGA TTCTGCTCTTCAGTTCATCTCAGAAATCATCCATCAGTTAACCAACCAAAAGGCCAGCATTGTGGATGAC ATCCACTCCACCTTTGATGAGCTGCAGAAGACTTTAAATGTTCGCAAGAGCGTGCTGCTCATGGAGTTGG AGGTCAACTATGGTCTCAAACACAAAGTGCTGCAGTCGCAGCTGGATACCCTGCTCCAAGGCCAGGAAAG CATTAAGAGCTGCAGCAACTTCACGGCTCAGGCCCTCAACCATGGCACGGAGACGGAGGTGCTGCTGGTG AAGAAGCAGATGAGCGAGAAGCTGAACGAACTGGCGGACCAGGACTTCCCACTGCACCCGCGCGAGAACG ACCAGCTGGATTTTATCGTGGAAACGGAGGGACTGAAGAAGTCTATCCACAACCTGGGGACCATCCTCAC CACTAACGCGGTCGCCTCTGAGACGGTGGCCACGGGGGAGGGGCTGCGGCAGACCATCATCGGGCAGCCC ATGTCCGTTACTATAACCACCAAGGACAAAGACGGAGAGCTGTGCAAGACAGGCAACGCATACCTCACCG CTGAGCTCAGCACCCCGGACGGCAGCGTGGCCGACGGAGAGATCCTGGACAACAAGAACGGCACCTACGA GTTCTTGTATACTGTGCAGAAGGAGGGGGACTTCACCCTGTCCCTCAGGCTCTACGACCAGCATATCCGA GGCAGCCCGTTTAAGCTCAAGGTCATCCGCTCTGCCGACGTGTCCCCCACCACCGAGGGCGTGAAGCGAC GAGTCAAGTCCCCGGGGAGCGGGCACGTCAAGCAGAAGGCTGTGAAGAGACCTGCCAGCATGTACAGCAC CGGCAAGCGAAAAGAGAACCCCATCGAGGACGACCTGATCTTCCGAGTCGGTACCAAAGGAAGAAACAAA GGAGAATTTACAAACCTGCAGGGTGTGGCTGCATCTACAAGTGGAAAGATATTAATTGCAGATAGTAACA ACCAGTGTGTGCAGATCTTTTCCAATGATGGACAGTTCAAAAGTCGTTTCGGCATCCGAGGACGATCACC CGGACAGCTCCAGCGGCCCACGGGGGTAGCGGTGCACCCCAGTGGGGACATCATCATTGCAGATTATGAC AACAAGTGGGTTAGCATTTTCTCCAATGATGGGAAGTTTAAGACAAAAATTGGATCAGGAAAACTGATGG GGCCCAAAGGAGTTTCTGTGGACCGCAATGGGCACATTATTGTGGTGGACAACAAGGCGTGCTGTGTGTT TATCTTCCAGCCCAACGGGAAAATAGTCACCAGGTTTGGTAGCCGAGGAAACGGGGACAGGCAGTTTGCA GGTCCCCACTTTGCAGCTGTGAATAGCAATAATGAGATCATCATTACAGATTTCCATAATCACTCTGTCA AGGTATTTAATCAGGAAGGAGAATTCATGTTGAAGTTTGGCTCCAATGGAGAAGGAAATGGGCAGTTTAA TGCACCAACAGGTGTGGCCGTGGACTCAAATGGGAACATCATTGTGGCTGACTGGGGGAACAGCAGGATC CAGGTTTTTGATGGGAGTGGATCATTTTTGTCCTATATCAACACATCTGCTGACCCGCTCTATGGCCCCC AGGGCCTGGCTCTCACTTCAGATGGCCATGTTGTGGTTGCAGACTCCGGAAACCACTGTTTCAAGGTCTA CCGGTACTTACAGTAACAGTGGACAGCTCTGTACTGAGGTCTTGCACTAGAAACTGGAATGGAGTTCTCA AGGTGGGACCAGACTACGATAGGACTTTTCATACTAGGAAGAATCTTCGGTGAACTTCCCAAGGTTATTT CTGAATGTAACCATTTCTTTAAAAACTGTCTATCCAATTTCCGTAATTCACTTTTAGGATTTAAAAAGAA TCTTCTTCTACTGAATCTATACAAACTGCGAAGTTTTACACCCATGAACTATGGCTCATAGGACAGGGAT TTCTGTAGTTAAACTAATTTTGCAAATCAGACGTTAAAAAGAATTAGCGTATCTGTTGACTGGTGAATGA ATGATAGCCTTTGCATAGAGCTTCCAAGAACAAAACTTGTAGTTGCCTATTTTATTTAACTTCGATCACA AGACCCAAGGGACCTTCTAACCTCACTTTTACAGTAGGTGTTATTATTCTGGTGACATGTTTTTGCTTAT CAACAACCACATATAAATTACCTTAGAAAAATCCAAGGCTGTCTTGCAAGATCCTTTCAGTTCAACTCAG GCCCTGAGTTGAGCCCATCAGAATCAATGCAAATTCCCCAAATTTCCTGGGTTTGATCAAAGATCGTTTC CATGTGTTCTCTTCACCTTCTCATTTCTGCTCACCTTGCATCAGAAAATGAAATATATTTTCAGGAAAAC CTGAAATTCCCGATGCTTTGGAAGGCACGTAGCAAAAGCAAGGGGACTCTTTGGTTACTAGCTTCAGATC AACACACTGGAATGGAATCAAGAGAAGTCACATTCATGTGCCATGCGTTCATGTGTCTTTCCTCTTCCAC ATCACGAAAGCGAAAGCTTTACCTCCTGCAAAACGTCAGCACATGTAGTCAGACGCCAGTGTCCTAGGAC AGAGAGCCATAAGCAGCCCTTTGGAGGATGGATGGTGTCAACCAAAGGCATGTGATTGATTCATGATTTC CCCTTAGAGAGCAAGTGTTACCAAAGTTCTGTTGAGCATTACAGGTAAGGGCATGTTGTGGTTATTTATC ATTGTCTAATGAGTAGTAGAGGCATTAAGGGGGCAAGTATACATGGTTAGGGTAAGATAGAATGTATTAT ACATATATATATATATATAAATATATAGCTGAGTCCTTGGTGTGCGGGAATAGGCAGCACTCTGAAAGAC AGAAGCATTGTCCAGCCATCCTTCAGTACATTCCCTGCCTGTTGCAGTCTGAAGCCGTCCCTGGAGAGTG AGCCCCACAGCAGCTTTCTGTGCTGCCATTTCCTGGCATGATGGTGATGTCTGGCTTCCAGTCAAATTAC CTTCTTTGTGTTCAAGAAAAAAAAGAAAGAAAGGAAGAAAAAAAGGAAGAAGGGGAGGAAGAAAGAAAGA AAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAGAAAGAGAGAAAGAAAGAAAGAAGTC TTTATAACATTGAGTTTGAGTTACCTTTCATTTAGGTATAAATGCATTTGAGAAACATTACCTGTCATGG ATACTGTAAACTAAGATGTGTTCTCTTTCCACTGATTTTCTACTCAGTTCTACCAAATAGGAATGTTGTG CTTTGCATGTTTGATGATGACGCTGTGATGTCATTCACCGAAAGCACCTTAGATATCTACTGTAAAAGAC ATCTTCCCTTAGATACAAATGATGATGTTTATGGCTTATTATCTTAAAATTCAAATGTTCTTGACATAAA TTTTTTAAAAAAATTTAGAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6826143 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6826143 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11835 same embryo
	EMAGE:11833 same embryo
	EMAGE:11836 same embryo
	EMAGE:11832 same embryo
	EurExpress:euxassay_008433 same experiment
	MGI:4828908 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source