EMAGE mouse gene expression spatial database

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EMAGE:11867

Palm3 paralemmin 3 ( MGI:1921587)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011110. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011110_01	euxassay_011110_02	euxassay_011110_03	euxassay_011110_04

euxassay_011110_05	euxassay_011110_06	euxassay_011110_07	euxassay_011110_08	euxassay_011110_09

euxassay_011110_10	euxassay_011110_11	euxassay_011110_12	euxassay_011110_13	euxassay_011110_14

euxassay_011110_15	euxassay_011110_16	euxassay_011110_17	euxassay_011110_18	euxassay_011110_19

euxassay_011110_20	euxassay_011110_21	euxassay_011110_22	euxassay_011110_23	euxassay_011110_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11867_wholemount_strong.wlz

11867_wholemount_moderate.wlz

11867_wholemount_weak.wlz

11867_wholemount_possible.wlz

11867_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11867_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 05 20 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 19 20

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 16 17 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 08 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 08 17 18 19

spinal cord

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 12 13 17 18 19

stomach

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10

midgut

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

bladder

moderate

regional

moderate expression: see section 14 15

metanephros

moderate

regional

moderate expression: see section 07 08 09 10 11 18 19 20 21

urethra of male

moderate

regional

moderate expression: see section 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37332
Entity Detected:	Palm3, paralemmin 3 ( MGI:1921587)
Sequence:	sense strand is shown >T37332 ATAGGGACATCTTCAGAAGCCAATGGCCCCTGCCCTGGGCACAGCCCTCTCCCAGAGCAGCTGAGTCTAG GGGTGTCGAGTGTCACTAAGGCCAAAGGAGATGGTGCAGTGGAGGTGGTTTGGGCTGGGCTTAGAGCCAC TGAGAACAGTGCCACAGGCCCCACAGATGTGGAGCTGGAGGCTAAGGTGGAAGAAGTGGTTCTGGAGGCC ATTGGGGCCAGGCAGGGCACCAGCAGCCCTGAGCTCCCCACTTGGGTGAAGGAGGGCAGGGGTGTAGTGG AAGTGGTCTGGGAGGGGCTGGGAGGCAGGGATCTTGATGTCACAGGGGAGTCAGGCAGGGATGCAGAGGC CACACACACCAGTTCCAGGAGACTCCAGGAGCAATTTGAGGCTGAAACTTGCAGAAAAGAAGAAGGTGCT TCCAGAGATAGTCTTGAGGGTGTTGGGCAGGGAGGTCCTGGAGTAGAGGAGGGATCCTTCATTTGGGTGG AGAGAGTGGCCCTCAGCGAAGACTGGGAGGAGATTTTGATGGAGGGTTTGGAAGCACCGCAGGGGGCAGG GTCTGCAGGAGAACCTGAGGCTTTGATTGGAGCACAGCCCAGAGGAGGTGAAGCGTCCTGGGAGGTAGAG AAGAGAGAAGTAGAGAAAGTTGAGGGCATAGAAGAGAAGGGAAGGGCAGAAAAGCTCGGGGCAGAGAGGG AGGATGGTGTAGCTGTGCTGCCAGATGAGACCCAGGGAAGAGAGGAAAACGAGGCAGAAAAGGTAGAGAG AAAAGACAGTGAGGGCCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 81433. Forward Primer - name:081433_F_cDNA_4432412L15Rik, sequence:ATAGGGACATCTTCAGAAGCCA; Reverse Primer - name:081433_N_SP6_cDNA_4432412L15Rik, sequence:GGGCCCTCACTGTCTTTTCTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11865 same embryo
	EMAGE:11866 same embryo
	EMAGE:11864 same embryo
	EurExpress:euxassay_011110 same experiment
	MGI:4822707 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source