EMAGE mouse gene expression spatial database

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EMAGE:11904

Itga7 integrin alpha 7 ( MGI:102700)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010969. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010969_01	euxassay_010969_02	euxassay_010969_03	euxassay_010969_04

euxassay_010969_05	euxassay_010969_06	euxassay_010969_07	euxassay_010969_08	euxassay_010969_09

euxassay_010969_10	euxassay_010969_11	euxassay_010969_12	euxassay_010969_13	euxassay_010969_14

euxassay_010969_15	euxassay_010969_16	euxassay_010969_17	euxassay_010969_18	euxassay_010969_19

euxassay_010969_20	euxassay_010969_21	euxassay_010969_22	euxassay_010969_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11904_wholemount_strong.wlz

11904_wholemount_moderate.wlz

11904_wholemount_weak.wlz

11904_wholemount_possible.wlz

11904_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11904_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

weak

spotted

weak expression: see section 02 03 04 21 22 23

upper leg muscle

weak

spotted

weak expression: see section 05 06 07 08 09 10 19 20 21 22 23

diaphragm

weak

spotted

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 19 20 21 22 23

pericardial cavity

weak

spotted

weak expression: see section 18

lower leg rest of mesenchyme

weak

spotted

weak expression: see section 04

vertebral axis musculature

weak

spotted

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

facial vii ganglion

moderate

regional

moderate expression: see section 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 08 09 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 09

dorsal root ganglion

moderate

regional

moderate expression: see section 10 11 12 13 14 15 18 19

tongue muscle

weak

spotted

weak expression: see section 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36526
Entity Detected:	Itga7, integrin alpha 7 ( MGI:102700)
Sequence:	sense strand is shown >T36526 TTTTACCTCATCCTCAGCACCTCTGGGATCACTATTGAGACCACAGAGCTGGAGGTGAAATTGCTGTTAG CCACGATCAGTGAGCAGGAGCTGGATCCGGTCTCCGTTCGGGCTCATGTCTTCATTGAATTGCCACTGTC CATTTCAGGGGTGGCCACTCCCCAGCAACTCTTCTTCTCTGGCGAGGTCAAGGGCGAAAGTGCCATGCGA TCTGAGAGGGAGCTGGGACGCAAAGTCAAGTATGAGGTCACGGTCTCCAATCAAGGCCAGTCTCTCAATA CTCTGGGCTCTGCGAACCTCAACATCATGTGGCCCCACGAGATCGCCAATGGAAAGTGGCTGCTGTACCC CATGCGGGTAGAGCTGGAGGGCGGACAGGGGCCTGGCAAGAGAGGGATCTGTTCCCCAAGACCCAACATC CTCCAGCTGGATGTGGACAGCAGGGATAGGAGGCGGCGAGAGCTGGGGCAGCCGGAGCCGCAGGAGCCTC CAGAGAAGGTGGAGCCTAGCACATCCTGGTGGCCAGTGTCCTCTGCTGAGAAGAGAAACATGACTCTGGA CTGCCCCAGGACGGCCAAGTGTGTGGTGTTCAGCTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 97954. Forward Primer - name:097954_F_cDNA_Itga7, sequence:TTTTACCTCATCCTCAGCACCT; Reverse Primer - name:097954_N_SP6_cDNA_Itga7, sequence:CAGCTGAACACCACACACTTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11906 same embryo
	EMAGE:11905 same embryo
	EMAGE:11907 same embryo
	EurExpress:euxassay_010969 same experiment
	MGI:4825645 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source