EMAGE mouse gene expression spatial database

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EMAGE:11915

Jak2 Janus kinase 2 ( MGI:96629)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008539. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008539_01	euxassay_008539_02	euxassay_008539_03	euxassay_008539_04

euxassay_008539_05	euxassay_008539_06	euxassay_008539_07	euxassay_008539_08	euxassay_008539_09

euxassay_008539_10	euxassay_008539_11	euxassay_008539_12	euxassay_008539_13	euxassay_008539_14

euxassay_008539_15	euxassay_008539_16	euxassay_008539_17	euxassay_008539_18	euxassay_008539_19

euxassay_008539_20	euxassay_008539_21	euxassay_008539_22	euxassay_008539_23	euxassay_008539_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11915_wholemount_strong.wlz

11915_wholemount_moderate.wlz

11915_wholemount_weak.wlz

11915_wholemount_possible.wlz

11915_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11915_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 01 02 03 05 06 21 22 23 24 moderate expression: see section 04 07 18 19 weak expression: see section 20

femur

weak

regional

weak expression: see section 03 04 05 23 24

thymus primordium

strong

regional

strong expression: see section 10 11 12 13 14

submandibular gland primordium

moderate

regional

moderate expression: see section 09 weak expression: see section 06 07 08 16 17 18 19

thyroid gland

moderate

regional

moderate expression: see section 10 14 weak expression: see section 13

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 09 10 11 12 13 14 15 17 18 19 20 21 22 23 24 weak expression: see section 08 16

vibrissa

strong

regional

strong expression: see section 20 21 moderate expression: see section 06 07 08 09 weak expression: see section 22

facial vii ganglion

weak

regional

weak expression: see section 04

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 06 weak expression: see section 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 05 06 07 21 weak expression: see section 04 08 16 17 18 19 20 22

vagus x ganglion

moderate

regional

moderate expression: see section 07 weak expression: see section 16

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 07 weak expression: see section 17 18

spinal cord

moderate

homogeneous

moderate expression: see section 07 09 10 11 12 13 14 15 weak expression: see section 08 16

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 09 10 11 15 17 weak expression: see section 08 16

neural retina

weak

regional

weak expression: see section 01 02 03 23 24

mandible

strong

regional

strong expression: see section 02 03 04 07 08 09 17 18 21 moderate expression: see section 05 06 16 19 20 22 weak expression: see section 10 11 15

maxilla

strong

regional

strong expression: see section 04 07 08 09 17 18 21 moderate expression: see section 05 06 19 20 weak expression: see section 10 11

liver lobe

strong

regional

strong expression: see section 01 02 03 moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

kidney calyx

moderate

regional

moderate expression: see section 10 11 18 weak expression: see section 06 07 17 20 21

kidney pelvis

moderate

regional

moderate expression: see section 09 19 weak expression: see section 08

thyroid cartilage

moderate

regional

moderate expression: see section 10 11 12 13 weak expression: see section 14

trachea

weak

regional

weak expression: see section 12 13

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 22 23 24 moderate expression: see section 05

clavicle

moderate

regional

moderate expression: see section 09 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9276
Entity Detected:	Jak2, Janus kinase 2 ( MGI:96629)
Sequence:	sense strand is shown >T9276 CTGAAGCAGGCGACGGGAACAAGATGTGAACTGTTTTCCCTCCCCAGAAGAAGAGGCCCTTTTTTTCCCT CCCGCGAAGGCCAATGTTCTGAAAAAAGCTCTAGATGGGAATGGCCTGCCTTACAATGACAGAAATGGAG GCAACCTCCACATCTCCTGTACATCAGAATGGTGATATTCCTGGAAGTGCTAATTCTGTGAAGCAGATAG AGCCAGTCCTTCAAGTGTATCTGTACCATTCTCTTGGGCAAGCTGAAGGAGAGTATCTGAAGTTTCCAAG TGGAGAGTATGTTGCAGAAGAAATTTGTGTGGCTGCTTCTAAAGCTTGTGGTATTACGCCTGTGTATCAT AATATGTTTGCGTTAATGAGTGAAACCGAAAGGATCTGGTACCCACCCAATCATGTCTTCCACATAGACG AGTCAACCAGGCATGACATACTCTACAGGATAAGGTTCTACTTCCCTCATTGGTACTGTAGTGGCAGCAG CAGAACCTACAGATACGGAGTGTCCCGTGGGGCTGAAGCTCCTCTGCTTGATGACTTTGTCATGTCTTAC CTTTTTGCTCAGTGGCGGCATGATTTTGTTCACGGATGGATAAAAGTACCTGTGACTCATGAAACTCAGG AAGAGTGTCTTGGGATGGCGGTGTTAGACATGATGAGAATAGCTAAGGAGAAAGACCAGACTCCACTGGC TGTCTATAACTCTGTCAGCTACAAGACATTCTTACCAAAGTGCGTTCGAGCGAAGATCCAAGACTATCAC ATTTTAACCCGGAAGCGAATCAGGTACAGATTTCGCAGATTCATTCAGCAATTCAGTCAATGTAAAGCCA CTGCCAGGAACCTAAAACTTAAGTATCTTATAAACCTGGAAACCCTGCAGTCTGCCTTCTACACAGAACA GTTTGAAGTAAAAGAATCTGCAAGAGGTCCTTCAGGTGAGGAGATTTTTGCAACCATTATAATAACTGGA AACGGTGGAATTCAGTGGTCAAGAGGGAAACATAAGGAAAGTGAGACACTGACAGAACAGGACGTACAGT TATATTGTGATTTCCCTGATATTATTGATGTCAGTATTAAGCAAGCAAACCAGGAATGCTCAAATGAAAG TAGAATTGTAACTGTCCATAAACAAGATGGTAAAGTTTTGGAGATAGAACTTAGCTCATTAAAAGAAGCC TTGTCATTCGTGTCATTAATTGACGGGTATTACAGACTAACTGCGGATGCGCACCATTACCTCTGCAAAG AGGTGGCTCCCCCAGCTGTGCTCGAGAACATACACAGCAACTGCCACGGCCCAATATCAATGGATTTTGC CATTAGCAAACTAAAGAAGGCGGGTAACCAGACTGGACTATATGTGCTACGATGCAGCCCTAAGGACTTC AACAAATACTTTCTGACCTTTGCTGTTGAGCGAGAAAATGTCATTGAATATAAACACTGTTTGATTACGA AGAATGAGAATGGAGAATACAACCTCAGCGGGACTAAGAGGAACTTCAGTAACCTTAAGGACCTTTTGAA TTGCTACCAGATGGAAACTGTGCGCTCAGACAGTATCATCTTCCAGTTTACCAAATGCTGCCCCCCAAAG CCAAAAGATAAATCAAACCTTCTCGTCTTCAGAACAAATGGTATTTCTGATGTTCAGATCTCACCAACAT TACAGAGGCATAATAATGTGAATCAAATGGTGTTTCACAAAATCAGGAATGAAGATTTAATATTTAATGA AAGTCTTGGCCAAGGTACTTTTACAAAAATTTTTAAAGGTGTAAGAAGAGAAGTTGGAGATTATGGTCAA CTGCACAAAACGGAAGTTCTTTTGAAAGTCCTAGATAAAGCACATAGGAACTATTCAGAGTCTTTCTTCG AAGCAGCAAGCATGATGAGTCAGCTTTCTCACAAGCATTTGGTTTTGAATTATGGTGTCTGTGTCTGTGG AGAGGAGAACATTCTGGTTCAAGAATTTGTAAAATTTGGATCACTGGATACATACCTGAAGAAGAACAAA AATTCCATAAATATATTATGGAAACTTGGAGTGGCTAAGCAGTTGGCATGGGCCATGCATTTTCTAGAAG AAAAATCCCTTATTCATGGGAATGTGTGTGCTAAAAATATCCTGCTTATCAGAGAAGAAAACAGGAGAAC GGGGAACCCACCTTTCATCAAACTTAGTGATCCTGGCATTAGCATTACAGTTCTACCGAAGGACATTCTT CAGGAGAGAATACCATGGGTACCTCCTGAATGCATTGAGAATCCTAAAAATCTCAATCTGGCAACAGACA AGTGGAGCTTCGGGACCACTCTGTGGGAGATCTGCAGTGGAGGAGATAAGCCCCTGAGTGCTCTGGATTC TCAAAGAAAGCTGCAGTTCTATGAAGATAAGCATCAGCTTCCTGCACCCAAGTGGACAGAGTTAGCAAAC CTTATAAATAATTGCATGGACTATGAGCCAGATTTCAGGCCTGCTTTCAGAGCTGTCATCCGTGATCTTA ACAGCCTGTTTACTCCAGATTATGAACTACTAACAGAAAATGACATGCTACCAAACATGAGAATAGGTGC CCTAGGGTTTTCTGGTGCTTTTGAAGACAGGGACCCTACACAGTTTGAAGAGAGACACTTGAAGTTTCTA CAGCAGCTTGGCAAAGGTAACTTCGGGAGTGTGGAGATGTGCCGCTATGACCCGCTGCAGGACAACACTG GCGAGGTGGTCGCTGTGAAGAAACTCCAGCACAGCACTGAAGAGCACCTCCGAGACTTTGAGAGGGAGAT CGAGATCCTGAAATCCTTGCAGCATGACAACATCGTCAAGTACAAGGGAGTGTGCTACAGTGCGGGTCGG CGCAACCTAAGATTAATTATGGAATATTTACCATATGGAAGTTTACGAGACTATCTCCAAAAACATAAAG AACGGATAGATCACAAAAAACTTCTTCAATACACATCTCAGATATGCAAGGGCATGGAATATCTTGGTAC AAAAAGGTATATCCACAGGGACCTGGCAACAAGGAACATATTGGTGGAAAATGAGAACAGGGTTAAAATA GGAGACTTCGGATTAACCAAAGTCTTGCCGCAGGACAAAGAATACTACAAAGTAAAGGAGCCAGGGGAAA GCCCCATATTCTGGTACGCACCTGAATCCTTGACGGAGAGCAAGTTTTCTGTGGCCTCAGATGTGTGGAG CTTTGGAGTGGTTCTATACGAACTTTTCACATACATCGAGAAGAGTAAAAGTCCACCCGTGGAATTTATG CGAATGATTGGCAATGATAAACAAGGGCAAATGATTGTGTTCCATTTGATAGAGCTACTGAAGAGCAACG GAAGATTGCCAAGGCCAGAAGGATGCCCAGATGAGATTTATGTGATCATGACAGAGTGCTGGAACAACAA TGTGAGCCAGCGTCCCTCCTTCAGGGACCTTTCCCTTCGGGTGGATCAAATCCGGGACAGTATAGCTGCG TGAAAGAGATGGCCTTCACTCAGAGACCAAGCAGACTTCCAGAACCAGAACAAAGCTCTGTAGCCTTGTG TCTACACATCCTTATCATGATGCTAGCTAGGCAGAAGAAACTGTGACGCCGTCTGCTCAAAGCTTTGGAA ACGCCGTGCGTTTGTTTCTACACCATCTGTAAAAACCACTGCTCAAGTCTGGCAGCATGCTTGTGGGCTG ATGCATGGAGCTCACCACAGAGTCTCTGCATCTCCTCTGGCAGAAGAAGAAAATAGACAGTTTTCAACTC AGCTTTTTTGAGAAATGGAAAAAATTATAATGTAAATTTTGCAGTGTAGGAAATACACAGAACATACATG TACAGTTTTTACCACGTGGAGTGTATAATACTTTGGCCTCTTGTGTGATTTACATGAGGGCTGATGTTTG TTAATGTTTTCTAATTTTTCCATAGGTGATCTATAATAACTTCATGATACAAATTAAAATGCTCAGAAAA TTAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6838318 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6838318 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4825676 same experiment
	EurExpress:euxassay_008539 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source