EMAGE mouse gene expression spatial database

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EMAGE:11925

Lama1 laminin, alpha 1 ( MGI:99892)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011017. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011017_01	euxassay_011017_02	euxassay_011017_03	euxassay_011017_04

euxassay_011017_05	euxassay_011017_06	euxassay_011017_07	euxassay_011017_08	euxassay_011017_09

euxassay_011017_10	euxassay_011017_11	euxassay_011017_12	euxassay_011017_13	euxassay_011017_14

euxassay_011017_15	euxassay_011017_16	euxassay_011017_17	euxassay_011017_18	euxassay_011017_19

euxassay_011017_20	euxassay_011017_21	euxassay_011017_22	euxassay_011017_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11925_wholemount_strong.wlz

11925_wholemount_moderate.wlz

11925_wholemount_weak.wlz

11925_wholemount_possible.wlz

11925_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11925_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 11 12

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11 12 13

diencephalon meninges

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

telencephalon meninges

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

hindbrain meninges

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain meninges

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

spinal cord ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13

spinal cord meninges

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14

cornea epithelium

moderate

regional

moderate expression: see section 05 weak expression: see section 23

lens

moderate

regional

moderate expression: see section 01

urinary system

moderate

regional

moderate expression: see section 03 04 21

maturing glomerular tuft

strong

regional

strong expression: see section 05 06 07 08 09 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36576
Entity Detected:	Lama1, laminin, alpha 1 ( MGI:99892)
Sequence:	sense strand is shown >T36576 AGGAATCGGAGAGTTATCACCATACAAGTGGATGAGAACAGTCCCGTAGAAATGAAGTTGGGTCCATTAA CAGAAGGAAAGACGATCGACATATCCAACCTGTACATAGGGGGACTTCCGGAGGACAAGGCGACCCCGAT GCTCAAGATGCGGACTTCGTTCCATGGGTGTATTAAAAACGTGGTCCTTGACGCTCAACTTTTGGACTTC ACCCATGCGACTGGCTCTGAGCAAGTAGAGCTGGACACATGCTTGCTGGCAGAAGAGCCCATGCAGAGTC TGCACAGAGAACACGGGGAACTCCCTCCGGAGCCCCCAACTCTACCACAGCCTGAACTGTGCGCAGTAGA CACGGCTCCGGGGTATGTGGCAGGTGCTCACCAGTTTGGCCTCTCGCAGAACAGCCACTTGGTGCTCCCT CTGAATCAGTCTGATGTCCGGAAGAGGCTCCAGGTGCAGCTGAGCATTCGGACATTTGCCTCCAGTGGCC TCATTTACTATGTGGCTCATCAGAACCAAATGGACTACGCCACGCTCCAGCTCCAAGAGGGCCGCCTGCA CTTCATGTTTGATCTCGGCAAGGGCCGGACCAAGGTCTCCCACCCTGCCCTGCTCAGTGATGGCAAGTGG CACACAGTCAAGACAGAATACATTAAAAGGAAGGCGTTCATGACTGTTGACGGCCAAGAGTCCCCCAGTG TGACTGTGGTGGGCAAT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 98039. Forward Primer - name:098039_F_cDNA_Lama1, sequence:AGGAATCGGAGAGTTATCACCA; Reverse Primer - name:098039_N_SP6_cDNA_Lama1, sequence:ATTGCCCACCACAGTCACACT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11928 same embryo
	EMAGE:11927 same embryo
	EMAGE:11924 same embryo
	EMAGE:11923 same embryo
	EMAGE:11926 same embryo
	EurExpress:euxassay_011017 same experiment
	MGI:4825857 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source