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EMAGE:11961

Itgb1 integrin beta 1 (fibronectin receptor beta) ( MGI:96610)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11961 EMAGE:11961 EMAGE:11961 EMAGE:11961 EMAGE:11961
"Pseudo-wholemount" of euxassay_010970. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010970_01 euxassay_010970_02 euxassay_010970_03 euxassay_010970_04
EMAGE:11961 EMAGE:11961 EMAGE:11961 EMAGE:11961 EMAGE:11961
euxassay_010970_05 euxassay_010970_06 euxassay_010970_07 euxassay_010970_08 euxassay_010970_09
EMAGE:11961 EMAGE:11961 EMAGE:11961 EMAGE:11961 EMAGE:11961
euxassay_010970_10 euxassay_010970_11 euxassay_010970_12 euxassay_010970_13 euxassay_010970_14
EMAGE:11961 EMAGE:11961 EMAGE:11961 EMAGE:11961 EMAGE:11961
euxassay_010970_15 euxassay_010970_16 euxassay_010970_17 euxassay_010970_18 euxassay_010970_19
EMAGE:11961 EMAGE:11961 EMAGE:11961 EMAGE:11961 EMAGE:11961
euxassay_010970_20 euxassay_010970_21 euxassay_010970_22 euxassay_010970_23 euxassay_010970_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11961Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11961_wholemount_strong.wlz
11961_wholemount_moderate.wlz
11961_wholemount_weak.wlz
11961_wholemount_possible.wlz
11961_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11961_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
regionalmoderate expression: see section 05 06 07 weak expression: see section 03 04 08 09 22 23 24
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 08 09 weak expression: see section 10 11 17 18 19 20
diencephalon floor plate
moderate moderate
regionalmoderate expression: see section 11 12 13 weak expression: see section 14
medulla oblongata floor plate
moderate moderate
regionalmoderate expression: see section 11 12 13 weak expression: see section 14
metencephalon floor plate
moderate moderate
regionalmoderate expression: see section 11 12 13 weak expression: see section 14
midbrain floor plate
moderate moderate
regionalmoderate expression: see section 11 12 13 weak expression: see section 14
spinal cord floor plate
moderate moderate
regionalmoderate expression: see section 13 weak expression: see section 14 15 16
aorta
weak weak
regionalweak expression: see section 13 14 15 16
esophagus
weak weak
regionalweak expression: see section 14 15
stomach
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 weak expression: see section 11 12 13
midgut
weak weak
regionalweak expression: see section 11 12 13 14 15 16 17 18 19 20 21 22
mandible
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 12 13 17 18 19 20 21 22 23
maxilla
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 17 18 19 20 21
bladder
weak weak
regionalweak expression: see section 15 16 17
lung
weak weak
regionalweak expression: see section 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22 23 24
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 21 22 23 24
clavicle
moderate moderate
regionalmoderate expression: see section 11 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36530
Entity Detected:Itgb1, integrin beta 1 (fibronectin receptor beta) ( MGI:96610)
Sequence:sense strand is shown

>T36530
GCAGAGCCTTCAATAAAGGAGAAAAGAAAGACACGTGTGCACAGGAGTGCTCCCACTTCAATCTCACCAA
AGTAGAAAGCAGGGAGAAGTTGCCCCAGCCGGTGCAGGTCGATCCTGTGACCCATTGCAAGGAGAAGGAC
ATTGATGACTGCTGGTTCTATTTCACCTATTCAGTGAATGGCAACAATGAAGCTATCGTGCATGTTGTGG
AGACTCCAGACTGTCCTACTGGTCCCGACATCATCCCAATTGTAGCAGGCGTGGTTGCTGGAATTGTTCT
TATTGGCCTTGCCTTGCTGCTGATTTGGAAACTTTTAATGATAATTCATGACAGAAGGGAATTTGCTAAA
TTTGAAAAGGAGAAAATGAATGCCAAGTGGGACACGGGTGAAAATCCTATTTACAAGAGTGCCGTGACAA
CTGTGGTCAATCCGAAGTATGAGGGAAAATGAATCCTACTCAGGCGGATTTTGCAACACCAAGCTCACAG
CAGCAGCATCTTAGTCACAGTAGGGTAGTTTTGGGGCTCTGTGGCCAGGGTTTTATTCACATGCAGGTTT
GGAAAATGTACAATATGTATAATTTTTAATTTTTTATTATTTTGAAAATAATGTTATAATCCATGCCAGG
GACTGACAGAAGACTTGAGAAAGGATCTTTATCCTTGTCAGCTGAGGTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 90121. Forward Primer - name:090121_F_cDNA_Itgb1, sequence:GCAGAGCCTTCAATAAAGGAGA; Reverse Primer - name:090121_N_SP6_cDNA_Itgb1, sequence:GACCTCAGCTGACAAGGATAAA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11962 same embryo
 EurExpress:euxassay_010970 same experiment
 MGI:4825651 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS