EMAGE mouse gene expression spatial database

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EMAGE:11962

Itgav integrin alpha V ( MGI:96608)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009584. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009584_01	euxassay_009584_02	euxassay_009584_03	euxassay_009584_04

euxassay_009584_05	euxassay_009584_06	euxassay_009584_07	euxassay_009584_08	euxassay_009584_09

euxassay_009584_10	euxassay_009584_11	euxassay_009584_12	euxassay_009584_13	euxassay_009584_14

euxassay_009584_15	euxassay_009584_16	euxassay_009584_17	euxassay_009584_18	euxassay_009584_19

euxassay_009584_20	euxassay_009584_21	euxassay_009584_22	euxassay_009584_23	euxassay_009584_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11962_wholemount_strong.wlz

11962_wholemount_moderate.wlz

11962_wholemount_weak.wlz

11962_wholemount_possible.wlz

11962_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11962_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

submandibular gland primordium

strong

regional

strong expression: see section 08 09 10 11 17 18 19 moderate expression: see section 20

brain

moderate

single cell

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 11 12 13 14

olfactory cortex ventricular layer

strong

regional

strong expression: see section 10 11 17

midbrain ventricular layer

strong

regional

strong expression: see section 11 12 13 14

spinal cord

moderate

single cell

moderate expression: see section 10 11 12 13 14 15 16 17

spinal cord ventricular layer

strong

regional

strong expression: see section 13 14 15 16

stomach

strong

regional

strong expression: see section 11 12 13 moderate expression: see section 09 10

midgut

strong

regional

strong expression: see section 21 22 moderate expression: see section 11 12 13 14 15 16 17 18 19 20

mandible

strong

spotted

strong expression: see section 05 06 07 08 09 10 11 12 13 17 18 19 20 21 22 23

lower jaw incisor

strong

regional

strong expression: see section 13 17 moderate expression: see section 14 15

lower jaw molar

strong

regional

strong expression: see section 08 09 19 20

maxilla

strong

spotted

strong expression: see section 07 08 09 10 11 12 13 17 18 19 20 21

upper jaw incisor

strong

regional

strong expression: see section 13 17 moderate expression: see section 14 15

upper jaw molar

strong

regional

strong expression: see section 08 09 19 20

kidney calyx

strong

regional

strong expression: see section 10 11 12 13 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36529
Entity Detected:	Itgav, integrin alpha V ( MGI:96608)
Sequence:	sense strand is shown >T36529 TAGCAAGACTGTCCTGTGCATTTAAGACAGAAAACCAAACTCGGCAGGTGGTGTGCGACCTTGGAAACCC GATGAAGGCTGGAACTCAACTGCTCGCTGGCCTTCGTTTCAGCGTGCACCAGCAGTCAGAGATGGATACT TCTGTGAAGTTTGACTTGAAAATCCAGAGCTCTAATTCCTTTGACAATGTAAGCCCAGTTGTGTCTTACA AGGTTGACCTCGCCGAAAAGGCTGCTGTGGAGATAAGAGGAGTTTCAAGTCCAGATCATATCTTTCTTCC AATTCCAAATTGGGAGTACAAGGAAAACCCTGAGACTGAAGAAGACGTTGGGCCTATTGTTCAGCACATA TATGAGCTGAGAAACAATGGTCCAAGTTCATTCAGCAAGGCAATTCTAAATCTCCAGTGGCCTTACAAAT ACAACAACAACACTCTGTTATACATTCTTCATTATGACATTGACGGGCCAATGAACTGCACGGCAGATAC AGAGATCAACCCTTTGAGAATCAAGACACCCGAAAAGAATGACACAGGGGCGGCGGGACAGGGAGAAAGG AGTCATCTCATCACCAAGCGGGGTCTCACCCTCAGAGAGGGAGATGTTCACACTTTGGGCTGTGGAATCG CCAAGTGCTTGCAGATCACCTGCCAGGTTGGTCGACTGGATAGAGGCAAGAGCGCAATCCTGTACGTGAA ATCCCTGCTGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 97974. Forward Primer - name:097974_F_cDNA_Itgav, sequence:TAGCAAGACTGTCCTGTGCATT; Reverse Primer - name:097974_N_SP6_cDNA_Itgav, sequence:ACAGCAGGGATTTCACGTACA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11961 same embryo
	EurExpress:euxassay_009584 same experiment
	MGI:4825650 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source