EMAGE mouse gene expression spatial database

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EMAGE:11964

Fam171b family with sequence similarity 171, member B ( MGI:2444579)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008581. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008581_01	euxassay_008581_02	euxassay_008581_03	euxassay_008581_04

euxassay_008581_05	euxassay_008581_06	euxassay_008581_07	euxassay_008581_08	euxassay_008581_09

euxassay_008581_10	euxassay_008581_11	euxassay_008581_12	euxassay_008581_13	euxassay_008581_14

euxassay_008581_15	euxassay_008581_16	euxassay_008581_17	euxassay_008581_18	euxassay_008581_19

euxassay_008581_20	euxassay_008581_21	euxassay_008581_22	euxassay_008581_23	euxassay_008581_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11964_wholemount_strong.wlz

11964_wholemount_moderate.wlz

11964_wholemount_weak.wlz

11964_wholemount_possible.wlz

11964_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11964_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex mantle layer

moderate

single cell

moderate expression: see section 11 12 13 16 17 18 19 20

olfactory cortex ventricular layer

moderate

single cell

moderate expression: see section 11 12 17 18

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 15 weak expression: see section 08 16

pons mantle layer

moderate

regional

moderate expression: see section 17 weak expression: see section 06

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 17 18

trigeminal v nerve

moderate

regional

moderate expression: see section 10 17 18

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 09 10 11 12 13 15 16 17 18 weak expression: see section 08

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 11 12 13 14 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36243
Entity Detected:	Fam171b, family with sequence similarity 171, member B ( MGI:2444579)
Sequence:	sense strand is shown >T36243 TTACAGGGACCGAAGAGGTCTATGGGCGGTCCCACATTCCCGAGCAGCTCATGCACATCTACAGCCAGCC TATTGCCATCCTTCAGACATCAGACCTTTTCTCCATGCCAGAGCAGTTACATGCCGCTAAGTCTGCCACT TTACCAAGAAAGGGACAATTGGTCTATGGCCAATTGATGGAACCAGTGAACAGAGAGAACTTTACACAAA CATTGCCCAAAATGCCGATGCATTCTCACGTCCAGGCCCCAGATGCCAGAGAAGAAGACATTGTACTTGA AGGTCAGCAGAGCTTGCCATCCCAGACCTCAGATTGGAGCCGATATTCCAACAGCTTGTTGGAGTCTGTG TCTGTTCCTGGAACTCTAAATGAAGCTGTGGTGATGACCCCCTTTTCATCGGAACTTCAAGGAATTTCAG AGCAGACCCTCCTGGAGCTGTCCAAAGGCAAGCCTCCGCATCCCAGGGCTTGGTTTGTGTCTCTTGATGG AAAGCCTGTGGCCCAAGTGAGACACTCCTTTATAGACCTGAAAAAAGGCAAGAGAACCCAGAGCAATGAT ACGAGTCTAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 68812. Forward Primer - name:068812_F_cDNA_D430039N05Rik, sequence:TTACAGGGACCGAAGAGGTCTA; Reverse Primer - name:068812_N_SP6_cDNA_D430039N05Rik, sequence:CTAGACTCGTATCATTGCTCTGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11968 same embryo
	EMAGE:11965 same embryo
	EMAGE:11967 same embryo
	EMAGE:11963 same embryo
	EMAGE:11966 same embryo
	EurExpress:euxassay_008581 same experiment
	MGI:4824720 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source