EMAGE mouse gene expression spatial database

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EMAGE:11980

Tbc1d8 TBC1 domain family, member 8 ( MGI:1927225)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011229. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011229_01	euxassay_011229_02	euxassay_011229_03	euxassay_011229_04

euxassay_011229_05	euxassay_011229_06	euxassay_011229_07	euxassay_011229_08	euxassay_011229_09

euxassay_011229_10	euxassay_011229_11	euxassay_011229_12	euxassay_011229_13	euxassay_011229_14

euxassay_011229_15	euxassay_011229_16	euxassay_011229_17	euxassay_011229_18	euxassay_011229_19

euxassay_011229_20	euxassay_011229_21	euxassay_011229_22	euxassay_011229_23	euxassay_011229_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11980_wholemount_strong.wlz

11980_wholemount_moderate.wlz

11980_wholemount_weak.wlz

11980_wholemount_possible.wlz

11980_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11980_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 24

upper leg muscle

moderate

regional

moderate expression: see section 03 04 05 06 07 23 24

diaphragm

moderate

regional

moderate expression: see section 05 06 07 22 23 24 weak expression: see section 02 03 04 08 09 10 21

forearm rest of mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04

lower leg rest of mesenchyme

moderate

regional

moderate expression: see section 02 03 04

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

thymus primordium

moderate

regional

moderate expression: see section 14 15 17 18 weak expression: see section 16

thyroid gland

weak

regional

weak expression: see section 14 18

vibrissa

weak

regional

weak expression: see section 06 07 08 09 10 22 23 24

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 18 19 20 21 22 23 24 weak expression: see section 02 13 14 17

telencephalon mantle layer

moderate

regional

moderate expression: see section 03 04 weak expression: see section 02

facial vii ganglion

strong

regional

strong expression: see section 06 07 08 23 24

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 09 21

trigeminal v ganglion

strong

regional

strong expression: see section 05 06 07 08 09 10 11 20 21 22 23 24

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 08 09 10 11 20 21 22

dorsal root ganglion

strong

regional

strong expression: see section 11 18 19 moderate expression: see section 09 12 13 17 20 21

cornea epithelium

moderate

regional

moderate expression: see section 01 05 06 weak expression: see section 02 03 04

naris

weak

regional

weak expression: see section 14 15 16 18 19

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 10 11 12 13 14 15 17 18 19 20 21 22

esophagus

moderate

regional

moderate expression: see section 15 16 17

tongue muscle

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 weak expression: see section 20

rectum

moderate

regional

moderate expression: see section 15 16

lower jaw incisor

moderate

regional

moderate expression: see section 14 15 18 19

lower jaw molar

moderate

regional

moderate expression: see section 09 10 22

upper jaw incisor

moderate

regional

moderate expression: see section 14 15 18 19

upper jaw molar

moderate

regional

moderate expression: see section 09 22

urinary system

weak

regional

weak expression: see section 04 05 06

bladder

moderate

regional

Expression in the fundus region.

renal cortex

weak

regional

weak expression: see section 08 09 10 11 12 19 20 21 22 23

testis

weak

regional

weak expression: see section 06 07 08 09

urethra of male

moderate

regional

moderate expression: see section 15 16

larynx

weak

regional

weak expression: see section 15 16 17

trachea

weak

regional

weak expression: see section 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6576
Entity Detected:	Tbc1d8, TBC1 domain family, member 8 ( MGI:1927225)
Sequence:	sense strand is shown >T6576 CCCACGCGTCCGCCCACGCGTCCGGCAGGGTGCCCCGCCAGGGCTGGCTGTACCTCAGCATCAACCACCT CTGCTTCTATTCCTTCTTCCTGGGCAAGGAGCTTAAACTTGTGATCCCCTGGGTCGATATCCAGAAGCTG GAGCGAACCTCCAATGTCTTTCTCACGGACACCATCCGGATCACTACACAGAATAAGGAGCGAGACTTCT CCACGTTTCTGAACCTGGACGAGGTGTTCAAGATCATGGAGCAGCTGGCAGATGTCACGCTCCGGAGGCT GCTGGACAACGAGGTTTTTGATCTTGACCCCGACCTGCAGGAGCCTAGCCAGATCACAAAGAGGGACCTG GAGGCCAGAGCACAGAACGAGTTCTTCCGGGCTTTCTTCAGGCTGCCGAGGGAGGAGAAGCTGCACGCTG TGGCAGACTGTTCCCTCTGGACGCCCTTCAGTCGCTGCCACACAGCCGGACGGATATTCTCCTCTGACAG CTACATCTGCTTTGCCAGCAGAGAGGATGGCTGCTGTAATGTTGTGCTTCCCCTGAGAGAGGTAGTGAGC ATTGAGAAGATGGAAGACACGAGCCTGCTGCCCAACCCCATCATCGTGAGCATCCGCAGCAAGATGGCCT TCCAGTTCATTGAGCTCAAGGACAGAGAGAACTTGGTGGAGGGCCTGCTGCTGAGACTGAAGCAGGTCCA CGCCAACCACCCTGTGCACTATGAGACCTCGCCAAGTGATGATGACATGGCTTCACCTGTGTTTTACTCA GCAAGCATCTGCACTGACAAGTTTGGGGATCTTGAGATGGTGGCTTCTCAAAGTAGCGAAGAGAGAGAGG AGAAGCGCCCCCTGCCACACCCCGAGCCCCTGACTGCCGTCTTCCAGCAGTCTGGCAGCCAGAGCCCCGA CTCCCGCCTGTCCCGGGAACAGATAAAAATCAGTCTGTGGAACGACCACTTTGTAGAGTACGGCAGGACC GTGTGCATGTTCCGCACTGAAAAGATCCGGAAGCTGGTGGCCATGGGGATCCCCGAGTCGTTACGTGGAA GGCTGTGGCTGCTTTTCTCAGATGCTGTGACGGACCTGGCCTCACACCCTGGGTACTATGGGAATCTGGT GGAGCAGTCTCTGGGGCGCTGCTGCCTGGTGACAGAGGAGATAGAGAGGGACCTGCACCGCTCTCTCCCT GAGCACCCCGCCTTCCAGAATGAAACTGGCATTGCTGCCTTGAGGCGGGTGCTGACAGCGTATGCCCACC GGAACCCCAAGATTGGATACTGCCAGTCCATGAACATCCTGACCTCTGTGTTGCTGCTGTATGCCAAAGA GGAGGAAGCCTTCTGGCTGCTGGTCGCTGTGTGTGAGCGGATGCTGCCTGATTACTTCAACCACCGGGTG ATTGGGGCTCAGGTGGACCAGTCTGTGTTTGAGGAGCTCATCAAGGAGCAACTCCCAGAGCTGGCGGAGC ACATGAGCGACCTCTCGGCGTTAGCGTCCATCTCCCTCTCCTGGTTCCTGACTCTGTTCCTCAGCATCAT GCCGCTGGAGAGTGCTGTGCACGTCGTTGACTGCTTCTTCTACGATGGGATCAAGGCCATCTTCCAGCTG GGGCTGGCTGTGCTGGAAGCCAATGCAGAGGAACTGTGCAGTAGCAAAGATGACGGACAGGCCCTGATGG TCCTCAGCAGGTTTCTAGATCACATTAAGAATGAAGACAGCCCGGGGCCCCCCATTGGCAGCCACCATGC CTTTTTCTCTGATGATCAGGAGCCATATCCTGTGACCGACATTGCTGACCTGATCCGGGACTCCTATGAG AAATTTGGAAACCAGTCTGTGGAGCAGATTGAACATCTACGCTGCAAGCACAGGATCAGGGTCCTGCAAG GACACGAGGACACCACCAAGCAGAATGTGCTCCGTGTCGTTATCCCAGAAGTCTCCATTCTTCCTGAAGA CCTTGAGGAACTCTATGACTTATTCAAGAGAGCACACATGATGAGCTGTTACTGGGAGCACCACAGGCCC ATGGCCCTGCGCCATGATCCCAGCAGGCCCTATGCTGAACAGTACCGCATCGATGCCCGGCAGTTTGCAC ACCTATTCCAGTTGGTATCGCCATGGACCTGTGGGGTCCATACAGAGATCCTTGCTGAGAGGCTCTTCAG GCTTCTGGACGACAACATGGACCAGCTCATAGAGTTCAAAGCCTTCACAAGCTGTCTAGACATCATGTAC AATGGAGAAATGAACGAGAAGATTAAGCTGCTGTACAGGCTTCATATCCCCCCAGCACTCACTGAAAATG ACAGAGATAGCCAGTCACCATTGAAGAATCCTCTGTTGTCAACTTCAAGACCCCTGGTCCTGGGCAAACC CAATGGTGACACAATTGACTATCAGAAACAGCTGAAACAGATGATTAAGGATTTAGCCAAAGAAAAGGAT AAAATGGAGAAAGAGTTGCCCAAAATGAGTCAGAGAGAATTTATCCAGTTCTGTAAAACTCTGTACAGTA TGTTCCATGAAGATCCAGAGGAAAATGACCTGTATCAAGCCATTGCCACAGTAACCACTCTGCTACTGCA GATTGGAGAAGTGGGCCAGCGAGGTAGCAGTTCAGGAAGCTGCTCCCAAGAATGTGAGGAGCCGCAGGCC TCGGCTCCTCCCGAACAGGACTCAGTTTTTGCTGAGGCTGGAAAGAGTCCCCAGGCCTTCCCCGAGACTG AAGGGGACTGGACTGTGTCCCTTGAACATATTTTGGCATCCCTTCTCACTGAGCAGTCACTGGTGAATTT TTTTGAAAAGCCACTCAACATTAAATCCAAACTTGAAAACGCCAAGCTCAATCAGTACAGTCTCAAAGTT TTGGAAATGAGCCACCCACCACAGGCAGAACTGAAGCTCAATGACCTGTAGGCCCTGAGCTGCTGGAGAA CACACTGGAGCCGACCACACCAAAGCAAGAGCTGTCAGCCTACAACCAGGGAGCTCCACCACCTCAGTCA CAGCTGCAGGTCTGACGCATTTCTATTTATTAAGAGGACAGGTGAGTGGTGGGACGCCCTGTTTACATAC ACTGTGACTATCAACTTTGCTGAGACCAGTGTTTCTGTTGTGATCCTGATAGTGAGTCTGCCCTTTGATG AAGAAAAAGATGCCACCGTAACTAAGAGACACCAACCCCAGGCTCATGTTTCCACTGGTCAGAAACAAGA GATCCCAGAACCATTGCCTGTACAACTGTATTTGGCTTTTTTTTGCACTAATTTTGTTTCAATGCTGGTA ATTGAAACCATTTAATATATCTCGTATTCACTTTATATGCCCTTACAAAAACATGTTCAAACCATGCCTT TAATGTTTGTCCTCAAGGTTTTTAATAAAAATCGTTCTTTTGTACTGAAAAAAAAAAAAAAAAAAAAAAA A
Notes:	The probe template was PCR amplified from IMAGE:3594100 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3594100 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11981 same embryo
	EurExpress:euxassay_011229 same experiment
	MGI:4828609 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source