EMAGE mouse gene expression spatial database

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EMAGE:11981

Kcna6 potassium voltage-gated channel, shaker-related, subfamily, member 6 ( MGI:96663)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011220. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011220_01	euxassay_011220_02	euxassay_011220_03	euxassay_011220_04

euxassay_011220_05	euxassay_011220_06	euxassay_011220_07	euxassay_011220_08	euxassay_011220_09

euxassay_011220_10	euxassay_011220_11	euxassay_011220_12	euxassay_011220_13	euxassay_011220_14

euxassay_011220_15	euxassay_011220_16	euxassay_011220_17	euxassay_011220_18	euxassay_011220_19

euxassay_011220_20	euxassay_011220_21	euxassay_011220_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11981_wholemount_strong.wlz

11981_wholemount_moderate.wlz

11981_wholemount_weak.wlz

11981_wholemount_possible.wlz

11981_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11981_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

hypothalamus mantle layer

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15 16 17 18

diencephalon lateral wall mantle layer

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 19

cerebral cortex marginal layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

telencephalon mantle layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

olfactory cortex marginal layer

strong

regional

strong expression: see section 12 13 14 17 18 19

medulla oblongata basal plate mantle layer

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 19

rest of cerebellum mantle layer

moderate

homogeneous

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

pons mantle layer

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

midbrain mantle layer

moderate

homogeneous

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

facial vii ganglion

strong

regional

strong expression: see section 06 07 08 09 21 22

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 10 19 20

trigeminal v ganglion

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 18 19 20 21 22

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 08 09 10 11 18 19 20 21

trigeminal v nerve

strong

regional

strong expression: see section 10 moderate expression: see section 12 18 19 20

spinal cord mantle layer

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17

dorsal root ganglion

strong

regional

strong expression: see section 09 10 11 12 16 17 18 19

neural retina

moderate

regional

moderate expression: see section 02 03 04 05

stomach

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11

tail mesenchyme

moderate

regional

moderate expression: see section 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9266
Entity Detected:	Kcna6, potassium voltage-gated channel, shaker-related, subfamily, member 6 ( MGI:96663)
Sequence:	sense strand is shown >T9266 GGCAGCCAGTTTCACGCGCGCGACAGTTTGCGGGTTCCAGGCATCTCAGAAATCCTGAGCACGGAGGCGC GGCTATTGAGAGCCAGAGCCACTTCCCAGACCTAGCCTGGCAGAGGGACCAGCTGCAGGGCTCACCGACC TAACCGCCAGGTCAGAGCACGGGCCCCACCCTAAAGGAGGGCACAGCCGAAGCTGTGAAGCGGGTGCCGC TCTCTGGAGCTCGTGTCGTGGGCGCCGTCCTAGTGGCGGGGAGCGCACCGCCGAGGTGACATGAGATCGG AGAAATCCCTGACGCTGGCGGCGCCGGGGGAGGTCCGTGGGCCGGAGGGGGAGCAACAGGATGCGGGTGA GTTCCAGGAGGCCGAGGGCGGCGGCGGCTGCTGTAGTAGTGAGAGGTTGGTGATCAACATCTCCGGGCTG CGCTTCGAGACGCAGCTGCGCACCCTGTCGCTGTTCCCTGACACGCTGCTAGGAGACCCTGGCCGCAGAG TCCGTTTCTTTGACCCCTTGAGGAACGAGTACTTCTTTGACCGCAACCGACCCAGCTTCGACGCTATCCT TTATTACTATCAATCTGGAGGTCGCCTGCGCAGGCCTGTTAATGTGCCCCTGGACATTTTTATGGAAGAG ATCCGCTTCTATCAGTTGGGAGAGGAAGCCCTGGCGGCCTTCCGGGAGGATGAGGGTTGCCTGCCCGAAG GTGGCGAGGATGAGAAACCACTCCCCTCCCAGCCTTTCCAGCGACAGGTCTGGCTTCTCTTTGAGTACCC GGAGAGTTCCGGGCCCGCCCGAGGCATCGCCATCGTCTCAGTATTGGTCATCCTCATCTCCATCGTCATC TTTTGCCTGGAGACCTTGCCTCAATTCCGTGCAGATGGGCGCGGTGGAAGCAACGAGGGTAGTGGGACCC GCTTGTCTCCGGCCTCCAGGAGCCACGAGGAAGAAGATGAGGATGAAGATTCCTATGCATTTCCTGGTAG CATTCCCTCTGGGGGGTTGGGGACTGGAGGAACATCTTCACTTAGTACTCTCGGGGGTTCTTTCTTCACA GACCCCTTCTTCTTGGTGGAAACTCTGTGTATCGTCTGGTTCACGTTTGAGCTCCTGGTGCGCTTCTCTG CCTGTCCCAGCAAGGCGGCCTTCTTTCGCAATATCATGAACATCATTGACTTGGTGGCCATTTTCCCCTA CTTTATCACCTTGGGCACCGAGCTAGTGCAACGTCACGAGCAGCAGTCTGTGAGTGGTGGCAGTGGTCAG AATGGGCAGCAGGCCATGTCCCTAGCCATCCTCAGGGTGATCCGCCTGGTCCGGGTGTTCCGAATCTTCA AGCTCTCCCGCCATTCCAAGGGGCTGCAGATCCTGGGTAAGACCTTACAGGCGTCCATGCGGGAGCTCGG GCTGCTCATCTTCTTCCTCTTCATCGGAGTCATCCTCTTTTCCAGCGCTGTCTACTTCGCAGAGGCTGAT GATGTTGACTCGCTCTTCCCTAGCATCCCAGATGCCTTCTGGTGGGCTGTGGTTACAATGACCACGGTAG GTTATGGGGACATGTACCCCATGACGGTAGGGGGCAAGATTGTGGGCTCACTGTGCGCCATTGCTGGGGT CCTCACCATTGCATTACCGGTACCGGTCATTGTCTCCAATTTCAACTACTTCTACCACCGAGAGACGGAG CAGGAGGAGCAAGGCCAGTATACCCACGTCACTTGTGGGCAGCCCACTCCGGACTTGAAGGCAACGGACA ATGGGCTTGGCAAACCTGACTTTGCCGAGGCTTCACGGGAACGCCGGCCCAGCTACCTTCCGACTCCACA TCGAGCTTATGCGGAGAAAAGGATGCTCACCGAGGTTTGATGGATGCGGGCAGGCCTGCAGGAAAACAGG AGCTCTGAGCAAGTCATCTCTCAGGCTTCCTTCTCATGCTCACTACTTCCGCCTTAGCTCCAGAGGACCT CGAACCCCCCTCCCCCCTAACACAGTACATGGCATCTTGGACCAAATATCTGGACTGTAGACTGTTGCTC GATCCTCGCAGCATTCGAGGTTTCTCCATCTTAGTGACATTTTGGAAATTTCAACGGTGCCACCCAATCA TGCCCATCAGTCACGTGGATGAGATGCACGTTTCTGACTGATCTTCCCTCAAGACTGAGTTTTCATGCCT GGGTCTTGTAATATCTCTAGGAATGGTGACACCAGTAGAATAGGAAGCAGCTACACTCAGGTCCCTTCTT TAGTGTCCTTTGCTTTGGGTCATGCACCTTCCTTACTTAGCTTCTGGTCACTTGGTCACTGGCAGAATCT GGAGAATGGAAAAGATTTCAGCTTGGCATTTTAGTGCCCAAGCCATCTTCTGACTGGTAGAGCTATTAGC TGCACGGCTTTGAAAGATATGGCTAAATTTGGATGGAATTAGAGAAAATACTACCCAACTGGATTATTTG AAGGACATGAAGGTCAGTTTTAGACCAATGGGGGCCGGTGGATTTCTCTAGTTGTGATCTGTCACAGGAG ATGCTGGCAGGGGTCTACATTAGTCCTCACCACTCTGATTCCATGACTTCCTTGCGCTTCCAGGAAGAAA CTTGGCACTTCCTCAGAGCCAAGTTCTTTTTATGTGCAAGCGCCTTCGTGTGCAGAGGAACTGGAAAATA GAACTACAGAGACAGGAGAAAAGGCTGACCAGAGCCAAAGGACTGGCTGGACCACATATCTGAAGCAAGG TGCCACTTAAGCAGAGACTGTCACTTAGAACAGGCAGAGGGACTCTGCTGCAGACAGCAGCCAATTCCTC CAGATTAGCTCAGACCTCTTTAAGATATCCTCCACCCTTTTTCCTGGGAATCTGACCTAGGATTGCAAAT GGATTTGTTTTTATAAAGCAACCTCAGCTGTAACCTACAGACAGGTGAGTTCACACATTGAATGCCAGCA TAGCTTTCCTCTTATGCCACAGGCAACAGCCTTTGGTTGGGAGCCAGGGAAGGGCACGGTCATTTTGTTG GCAGCCTCTCTCTTAGAGGATTTTGCTGAGTCAAGCAAACGTTTGTCTGCTCAGCTCTTCCTTTGGAGCT GCATTGGGCCTGCTAAGGTAACTCGGTCGTTCCTACTTGGACCAAGCCATCTTCTCCCATTGTGAAATCA CTGCCACCCTCCCAGCTGCCCACTGCCCCCTCCCCCAGTAACATTGCCATTCAGTTTTGCCTTTGATAAA CTGTGATACACTCTTCTCAGTTCTCATGGGTACAGCTCTGAAACTCAAAGGACTGTTAACATGTTTTTAA TTTTCTATTGATGCTTTAAGACTCTTCGAAGGGAATGGCTTTTCAAAAGATTTTTTTTTTCCCTGAAGAA CAACTTAGGAAGGAGTGACCTTGATGGTTGGCTTGAGAGGCCACTGTGGCTCCACTCTGAGTTTTGCCAC AGCTTTACAGGGTAACTCTGGTGTCATGGTCCCTCTTTGTGCCTCAGTTCCCCCCACCCATTAAATGAGA ACATTAATGTCTTCGCCTCCTTACCTCATGGGGAATTATCAATAAACTCACCCAGGAGGGTGGGGAGGAT GCTATGCAAATGTGTGAATTATAGTAATGGCCTTGAGTTTTAGTTCACTATACTGCCTGCCTATCTCTCA AAGGCATGGCCTTTCCTCAGAACTTTCTACCATGGATCCAAAGCCTAAGACTAGGCCATCGTAGACCATC AGCTCTCTAAGTCGCTCCCTGCCATCTTGGGCTGGGTCTCTCCATCCTTTCTCTCACCCGTCAGAAAAGG AGTGTATTCTTGCATTAGACTTGGTGGCCAGAAGTCACCTTATCTGGGAGCTTAACTTCTCCAAGCCTGC AACATAGTAAAAGGCTGAGATGGTTGAGACTCAGAGATCTATTCTAAGGCCAGTAAGAAAGTACTTTCTG TTTCCAATGATTTTAGTTACTCCATGCCCACCCCAGTGGCCAAAGGGATGTCTCCAGCTGAGGGTGGATG GGGGGACATGTGTGCATGGACCTCTGTGTATGTATAGATTGTCACCCTCATCACTGTCCCTGTCCTTATG CAAACATTGCCTTTTCAAGTCAGAGCATGCCTGAAAGTAAAAGGTTTGTGGAGAGCCAGGCTCTCCAGGG ACTCTGGTTGCTGTAGCAACCTTCCAGACTGTTCTCTCTACTGATTTGGGTATCAAGGGAGAACAGACTA CATATCCCAGCTTCTCACACGGGAACACCTCCAATCTATTGGTTTAGGCATTGGCAGAACTAAAATAAGG ACTCAGGACCTTTGGCAATGTGGAGGGAGTAGATTCCCAACACTTCCTGCCTAGACCTTTCTTCCTGGTT CCCTGTATGGTCATGGAAGCCGCCACATCTTACTCCGAGTCTTCAGAAACTTGAATGCTTAAAACCTGCC CAATTCAGCCAGAAGCAAGGTTAAGGTTTTCAAAGGAAGACCTCAGAGAGGGGTATAAAATGTCCCCTCC ATCGCAGCCAGACTGGGCCTTGAGGACACAGATGACACCAATCTGGGATGTCATGGTATTCTCATCTCAG GCGCAGGTTCTTCCCAGCCCTAAGAGAGATCAAGAAGGATGAAACTCCTCCCTCCCAGGGAGCAACACTG CTCCTGGCAAAATTACAGAAGTGATTCTTAAGATGTCAGACAGAAACTGAGAGGCTGTGACTATCAAGTT TTTGTGGAGAATTTTGTCCAGTGAGCTCTCCCTGCTTCTGAGCCTCCATGTGGATGATGTAACCGTGTCA TCAAGAGACCATGCACCACCTTGTGCTGCCTCAACAGCTGGATCAATAGGACCACAGCTGCTCAAGAAGG TGGGGTTCAAGCCATCTCGATGACTGACGTAAGCCAGTGGGGGCTCCCAGAGGATACTCTGGGACTGCCA ATTTCTGCTTGGGATGAGGACAGCCCTCCTGCCTAGCATCCCAGAGGAGCAAGGGACAGCTGGCCTTTCT TGCCTCTGAGGGTTGTGCTGCACGAGGCCACTCTGGAAAACCTATGCATCCAAAGAGTACCCGATCTTGA ACTTCTCTGGAGGATGAGACCCCCGCCTCCCCACTGAATAACTCAGCCGGACAGCCCCTGGTGGCCTTCC ACAAGCAGTGACTGAGACGGAAGGAAAAGGAAAAGGCTGAGATGGGGGGGGGGGCTCCTTGCCAGTGAAC GATGGGATTCTGAGTGTTGGGGTGGGGCCAGAGGAGGAGAAGGAGAGTTGCTGGTCTGATCAAAGGAGAG GAGGTGTTAGGATGATCTGGGATCGGAAGAATTCCTGAGTGTGCTGCAGAGTCGCCCAGGCACTCGCTGG CTGAGGAAGAGTAACTAATAAATAAATGACAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:6838056 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6838056 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11980 same embryo
	EurExpress:euxassay_011220 same experiment
	MGI:4825698 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source