EMAGE mouse gene expression spatial database

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EMAGE:12007

Cotl1 coactosin-like 1 (Dictyostelium) ( MGI:1919292)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010951. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010951_01	euxassay_010951_02	euxassay_010951_03	euxassay_010951_04

euxassay_010951_05	euxassay_010951_06	euxassay_010951_07	euxassay_010951_08	euxassay_010951_09

euxassay_010951_10	euxassay_010951_11	euxassay_010951_12	euxassay_010951_13	euxassay_010951_14

euxassay_010951_15	euxassay_010951_16	euxassay_010951_17	euxassay_010951_18	euxassay_010951_19

euxassay_010951_20	euxassay_010951_21	euxassay_010951_22	euxassay_010951_23	euxassay_010951_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12007_wholemount_strong.wlz

12007_wholemount_moderate.wlz

12007_wholemount_weak.wlz

12007_wholemount_possible.wlz

12007_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12007_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

thymus primordium

moderate

regional

moderate expression: see section 11 12 13 14 15

thalamus mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

thalamus ventricular layer

moderate

regional

moderate expression: see section 12 13

cerebral cortex mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

cerebral cortex marginal layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

medulla oblongata alar plate mantle layer

strong

regional

strong expression: see section 07 11 17

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 08 09 10 15 16 17

lens

strong

regional

strong expression: see section 01

neural retina

strong

regional

strong expression: see section 01 02 03 23 24

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

vomeronasal organ epithelium

strong

regional

strong expression: see section 11 14

esophagus epithelium

moderate

regional

moderate expression: see section 12 13

pharynx epithelium

moderate

regional

moderate expression: see section 11 12 13

stomach

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09

midgut

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

renal cortex

strong

regional

strong expression: see section 07 08 09 10 17 18 19 20

trachea

moderate

regional

moderate expression: see section 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36187
Entity Detected:	Cotl1, coactosin-like 1 (Dictyostelium) ( MGI:1919292)
Sequence:	sense strand is shown >T36187 CAGATTACCAGCACTTCATCCAGCAGTGCACAGATGATGTCCGGCTGTTCGCCTTTGTGCGCTTCACCAC CGGCGACGCCATGAGCAAAAGATCCAAGTTTGCCCTCATCACATGGATCGGGGAGGACGTGAGTGGACTG CAGCGTGCCAAGACGGGGACCGACAAGACGCTGGTGAAGGAGGTGGTCCAGAATTTTGCAAAGGAATTTG TGATCAGCGACCGGAAGGAGCTAGAGGAAGACTTCATCAGGAGCGAGCTGAAGAAGGCTGGGGGGGCCAA CTACGACGCTCAGTCAGAGTAGCTACAGCCCCACAGCCCAGAGCCATCTGCCTGCTCCGCCAAGGAGGAG ACTGCCGCCTCACGTCACCAGCCCACCAGGGAGGAGAAGCCATGAGAGGCACCAGCGCCACCAGTGTGTC CAACCCTCCCCTTCCCTCTCCCCCCTGTGGCCCCTCTCAAGCTCACCAAGCATCATTCTTTGAGACCCGC CCCCCCTTTTGTTTTAAAGAAGTCATTTTGGTGCCAGGGTCATGCATACCATCTGATCCCAGAAGCCTCT CTGTCACTGGCCCTGGACCTGGCATTTACCCCTGCCGTCACCGTGGCCTGCCTGGTCTAAGCTACCCTGG CCTTGAGTTGCTGAGGCCTGGTCAGGTGCCACCCCAGCTTGACCTGGCTGTTTCCTGCTAGCCCTACGCC GGCCACCAATACCAGGAGCTGTGCACACCTTACAGCCTTCATGAGCTCCCATGCGCGCGCATGCTCCTCA GAGCCCATAGTTGTGGCCCCAGGCCTCACCCCTGTCCTGTTCAGAGTGGCCTTGAGCTGTCCCTCTGGTA GACAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 91451. Forward Primer - name:091451_F_cDNA_Cotl1, sequence:CAGATTACCAGCACTTCATCCA; Reverse Primer - name:091451_N_SP6_cDNA_Cotl1, sequence:GTGTCTACCAGAGGGACAGCTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12010 same embryo
	EMAGE:12006 same embryo
	EMAGE:12009 same embryo
	EMAGE:12005 same embryo
	EMAGE:12008 same embryo
	EurExpress:euxassay_010951 same experiment
	MGI:4824014 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source