EMAGE mouse gene expression spatial database

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EMAGE:12025

Gpr125 G protein-coupled receptor 125 ( MGI:1917943)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008255. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008255_01	euxassay_008255_02	euxassay_008255_03	euxassay_008255_04

euxassay_008255_05	euxassay_008255_06	euxassay_008255_07	euxassay_008255_08	euxassay_008255_09

euxassay_008255_10	euxassay_008255_11	euxassay_008255_12	euxassay_008255_13	euxassay_008255_14

euxassay_008255_15	euxassay_008255_16	euxassay_008255_17	euxassay_008255_19	euxassay_008255_20

euxassay_008255_21	euxassay_008255_22	euxassay_008255_23	euxassay_008255_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12025_wholemount_strong.wlz

12025_wholemount_moderate.wlz

12025_wholemount_weak.wlz

12025_wholemount_possible.wlz

12025_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12025_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 10 11 15 16 17 19 20 21 22 23 moderate expression: see section 12 13 14

forearm mesenchyme

strong

regional

strong expression: see section 01 02 03

upper arm mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 21 22 23 24 moderate expression: see section 07

hand

strong

regional

strong expression: see section 02 03 04 05 21 22 23 24

foot

strong

regional

strong expression: see section 02 03 04 05 19 20 21 22 23 24

lower leg mesenchyme

strong

regional

strong expression: see section 01 02 21 22 23 24

upper leg mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 19 20 21 22 23 24

submandibular gland primordium

strong

regional

strong expression: see section 08 09 10 11 12 19 20 21

vibrissa

strong

regional

strong expression: see section 06 07 08 09 10 22 23 24

otic capsule

strong

regional

strong expression: see section 08 09 10 17 19 20

cochlea

strong

regional

strong expression: see section 08 09 10 17 19 20 21

utricle

strong

regional

strong expression: see section 05 06 07 08 21 22 23 moderate expression: see section 24

nasal septum

strong

regional

strong expression: see section 15 16 17

naso-lacrimal duct

strong

regional

strong expression: see section 05 06 07 08 09 10 11 20 21 22 23 24

viscerocranium

strong

regional

Expression in the turbinate bone.

tongue muscle

strong

regional

strong expression: see section 15 16 moderate expression: see section 13 14 17

mandible

strong

regional

strong expression: see section 04 05 06 07 08 09 10 13 14 15 16 17 19 21 moderate expression: see section 02 03 11 12 20 22 23 24

lower jaw incisor

strong

regional

strong expression: see section 13 14 15 16 19

lower jaw molar

strong

regional

strong expression: see section 08 09 20 21

maxilla

strong

regional

strong expression: see section 04 05 06 07 08 09 10 13 14 15 16 17 19 21 moderate expression: see section 20 22 23 24

upper jaw incisor

strong

regional

strong expression: see section 13 14 15 19

upper jaw molar

strong

regional

strong expression: see section 08 09 20 21 22

renal cortex

strong

regional

strong expression: see section 06 07 08 09 10 17 19 20

axial skeleton

strong

regional

strong expression: see section 09 10 11 16 moderate expression: see section 12 13 14 15

basisphenoid bone

strong

regional

strong expression: see section 13 14 15 16 17

temporal bone petrous part

strong

regional

strong expression: see section 05 06 21 22 23 moderate expression: see section 02 03 04 24

orbito-sphenoid

strong

regional

strong expression: see section 02 03 04 05 07 08 09 10 21 22 23 moderate expression: see section 24

sternum

strong

regional

strong expression: see section 15 moderate expression: see section 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35189
Entity Detected:	Gpr125, G protein-coupled receptor 125 ( MGI:1917943)
Sequence:	sense strand is shown >T35189 AAGTCACCAAGAAAGCCAAGAGATGCCAGGATCCAGATGAGCCACCCGCTCCTCCACGACCGATGCTGAG GTTCTACCTGATTGGTGGTGGGATCCCCATCATAGTGTGTGGTATCACCGCGGCAGCAAACATCAAGAAC TATGGCAGTCGGCCCAGTGCACCGTATTGCTGGATGGCCTGGGAACCGTCCTTGGGAGCCTTCTACGGAC CTGCCAGCTTCATCACTTTTGTAAACTGTATGTATTTTCTAAGCATATTTATTCAGTTGAAAAGACACCC TGAGCGCAAATATGAGCTCAAGGAGCCGACAGAAGAGCAGCAGAGATTGGCAGCCAATGAAAATGGTGAA ATCAACCATCAGGACTCCATGTCTCTGTCTCTCATCTCTACGTCCACGTTGGAGAACGAGCACAGTTTTC AGTCTCAGCTTCTGGGCGCCAGCCTTACTTTGCTTTTGTATGTCATCTTGTGGATGTTTGGGGCCATGGC TGTTTCTCTGTATTACCCTCTGGACTTGGTTTTTAGCTTCTTCTTCGGAGCCACTTGTTTAAGCTTCAGT GCTTTCATGATGGTGCACCACTGCATCAACAGGGAGGACGTGAGACTTGCGTGGATCATGATGTGCTGCC CAGGGCGGAGCTCGTACTCCGTGCAAGTCAACGTCCAACCTCCCAACTCAAGCGCCACTAATGGAGAGGC TCCAAAGTGCACCAATAGCAGCGCAGAGTCTTCGTGCACGAACAAAAGCGCATCGAGCTTCAAAAACTCT TCCCAGGGCTGCAAGCTGACAAACTTGCAGGCTGCTGCGGCACAGTACCACAGCAATGCCCTACCTGTGA ATGCCACGCCGCAGCTTGATAACAGTCTGAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 78738. Forward Primer - name:078738_F_cDNA_Gpr125, sequence:AAGTCACCAAGAAAGCCAAGAG; Reverse Primer - name:078738_N_SP6_cDNA_Gpr125, sequence:GTCAGACTGTTATCAAGCTGCG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12022 same embryo
	EMAGE:12026 same embryo
	EMAGE:12027 same embryo
	EMAGE:12024 same embryo
	EMAGE:12023 same embryo
	EurExpress:euxassay_008255 same experiment
	MGI:4825171 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source