EMAGE mouse gene expression spatial database

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EMAGE:12148

Card10 caspase recruitment domain family, member 10 ( MGI:2146012)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011116. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011116_01	euxassay_011116_02	euxassay_011116_03	euxassay_011116_04

euxassay_011116_05	euxassay_011116_06	euxassay_011116_07	euxassay_011116_08	euxassay_011116_09

euxassay_011116_10	euxassay_011116_11	euxassay_011116_12	euxassay_011116_13	euxassay_011116_14

euxassay_011116_15	euxassay_011116_16	euxassay_011116_17	euxassay_011116_18	euxassay_011116_19

euxassay_011116_20	euxassay_011116_21	euxassay_011116_22	euxassay_011116_23	euxassay_011116_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12148_wholemount_strong.wlz

12148_wholemount_moderate.wlz

12148_wholemount_weak.wlz

12148_wholemount_possible.wlz

12148_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12148_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

other

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

esophagus

strong

regional

strong expression: see section 14

lower jaw incisor

strong

regional

strong expression: see section 13

lower jaw molar

strong

regional

strong expression: see section 08

upper jaw incisor

strong

regional

strong expression: see section 13

upper jaw molar

strong

regional

strong expression: see section 08

urinary system

strong

spotted

strong expression: see section 05 06 07 23 24

metanephros

strong

regional

strong expression: see section 07 08 09 10 11 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9383
Entity Detected:	Card10, caspase recruitment domain family, member 10 ( MGI:2146012)
Sequence:	sense strand is shown >T9383 GCGCGGCTGGCCCGCAGAGCGGCCGGGAGGACGACCGGAGCGCAGCGGGTCGCGGGCAATGTTGGGTGGC CCGGGAGGCCGCCGGGCTGGGGGCGGAGCCAATGACCCCGAAGGCACCGCCCCGTCGCGCCTCCGGAGCC GTCGGCCGAGCACAGAGCCGGGAGCTCAGGGCAGCTGCAGCGCCCGTGGCTCGGAGGACGCGGCCATGCA GGGCCGGGCGGACGCGGGCGAGGCTGACGAGGAGGCTGGGGCGGGTTCGGGGTCGGAGGCCGAAGAGGAC GCGCTGTGGGAGCGGATCGAGGGCGTCCGGCACCGACTGACGCGCGCCCTGAATCCGGCCAAGCTCACGC CGTATCTGCGCCAGTGCCGCGTCCTGGACGAACAGGACGAGGAGGAGGTGCTCAGCACCTACCGTTTCCC GTGCCGCGCTAACCGTACCGGGCGCCTCATAGACATTTTACGCTGCCGGGGCAAGAGGGGCTTCGAGGCC TTCCTGGAAGCCCTGGAATTCTACTACCCAGAACACTTCACGCTGCTCACTGGCCAGGAACCTGCCCAAC GCTGCTCCATGATCCTTGATGAGGAGGGTCCCGAGGGCCTGACTCAGTTCTTGATGACTGAGGTCCGGAG GCTTCGGGAAGCTCGGAAGAGCCAATTGCACCGAGAGCAGCAACTGCAGGCCCGGGGCCGGGCCCTGGAG GAGGAGCGGGCAGGGCTAGAGCAGAGGCTTCGGGAGCAGCAGCAGGCACAGGAGCGCTGCCAGCGACTGC GGGAGGACTGGGAGGCTGGCAGCCTGGAGCTGCTGCGCCTCAAGGATGAGAACTACATGATCGCCATGCG CCTGGCTCAGCTCAGCGAGGAGAAGAATTCGGCTGTGCTGCGCAGCCGAGACCTGCAGCTGGCGGTGGAC CAGCTCAAGCTCAAGGTCAGCCGGCTGGAGGAAGAGTGTGCGCTGCTGAGGAGGGCCAGGGGCCCACCGC CTGGGGCTGAGGAGAAGGAGAGGGAGCCAGATGGCGCTGATCTTCTCTCTGAGCTTCGTGCAGAGAACCA GCGGCTGACGGCTTCACTGCAGGAGCTTCAGGAAGGTCTTCAGCAGGAGATGAGCCGGCCAGGAGCTGCA GGCTCTGAACGTATCCTACTGGACATACTGGAGCACGACTGGAGGGAGGCGCAGGACAGCAGGCAGGAGC TGTGTCAGAAGCTTCATGCTGTGCAGGGCGAGCTACAGTGGGCAGAGGAGCTGCGGGACAAGTACCTGCA AGAGATGGAGGATCTGCGGCTCAAACATCGAACGCTGCTGAAGGACTGTGACCTCTATAAGCACCGCATG GCCACTGTGTTGGCCCAGCTGGAGGAGATTGAGAAAGAGCGGGACCAGGCAATCCAGAGCCGAGACCGGA TCCAGCTGCAGTATTCTCAGAGCCTCATTGAGAAGGACCAGTACCGCAAGCAGGTGCGGGGGCTGGAGGC GGAGCGCGATGAGCTGCTCACCACAGTCACCAGCCTGGAGGGGACCAAGGCCATGCTGGAGGCACAGCTG CAGCGGACACAGGGGGGTTCCTGTCTCAAGGCCTGTGCCTCCTCCCACTCCCTGTGCTCCAACCTCAGCA GCACCTGGAGCCTCAGCGAGTTCCCATCGCCCCTGGGAGGCCCAGAAGCTACAGGAGAAGCCGGGGGATC TGAGCCCCACACCTCGGAGGAAGCCACAGACAGCGAGAAGGAGATCAACCGGCTCTCTATCCTGCCTTTC CCACCCAGTGCTGGTTCCATACTCCGCCGGCAGCGTGAGGAAGACCCGGAGCCCCCTAAGAGGTCTTTTA GCAGCATGTCGGATATCACAGGGAGTGTGACCCTTAAGCCATGGTCCCCTGGCCTCTCTTCATCATCCTC GTCTGACAGTGTGTGGCCTTTGGGAAAGCCGGAAGGCCTCCTGGCTCGGGGCTGTGGCCTGGACTTCCTC AATAGGTCTCTGGCCATCCGAGTGTCCGGCTGGAGTCCCCCCGCAGGCCTGGATCCCCAGGATAAGAGTC CAGATAGCATGCCGGGCCTTGGAGACAGGTGGTCTGGGGCGGTGGTGCGAAGGGTGTTGTCTGGGCCTGG GTCAGCCAGGACAGAACAGAAAGAGCCGAGGGCGGAAGGCACTGGTCTGGAGGGGGCAGGCCTGGAAGCG GAGGCCCAGCAGAGGACCTTGCCCTGGAACCAGAGCTCCACCCTCCCCTTCCTGCTGGACTCAAAGGCCT GTCATTCCTTCCATGAGGCCCTGGATGCCTGGGCAAAGGGACCGGGTGCCGAGCCCTTCTACATTCGAGC CAACTTCTCCCTGCCCGAGCGGTCGGATCCCCATGCCCTTTGTGTGAAAGCCCAAGAGATCCTGCGGTTG GTGGACCCAGCCCACAAGCGGAGGCAGGAGTGGTTCTGTACTCGAGTGGACACCCTCACCTTACGGGACC TGGACCGGGGCACCGTGCCCAATTATCAGAGAGCTCAGCAGCTCCTAGAAGTCCAGGAAAAATACTTGAT CTCCAGCAGACACCGAAGTCCTCGAAGCAACCTGAAGAAGCGAGCCCTAGGGTTGGTGAGGCCCAAGCCT GCAGGGGGCACTGCAGGAGACTCAGCAGAACAGCTCCCTGCAGAGCCCTGCTCAGAGCTAGAACGAAGCC TAAAACCCTACAGCCTGGTGCGACCGCTGTTGGTGTCTGCCTTGAGGCCTGTGGTCCTCCTGCCTGAGTG CCTGGCGCCTCGCCTCATCCGTAACCTGCTGGATCTGCCAAGCTCCAGGCTGGATTTCCAAGTGTGCCCA GCAGAGAGCCTCTCTGGGGAGGAACAGTGTACGTCCTCAGCTCCCGGAGCCCCCAAAGCCTGGCCGGCCA CTGCTGGGCTAGGCAGCAGGATCCGTGCCATCCAGGAGTCTGTCGGGAAGAAGCACTGCCTGTTGGAGCT GGGTGCACGGGGAGTGCGGGAGTTGGTGCACAGCGAGGTCTACCCCATTGTCATCCACGTGGAGGTGACT GAGAAGAATGTCCGTGAGATCAGGGGCCTGTTGGGCCGGCCGGGCTGGCGGGATTCAGAACTGCTCCGTC AGTGTCGGGGCTCAGAACAGTGGCTCTGGGGACTGCCGTGTTCCTGGGTGCAGGTGCCTGCGCATGCGTG GGGCCATGCAGAGGAGCTGGCCAAGGTGGTGCGTGGCCGAATCTTGCAGGAACAGGCTCGCCTTGTGTGG GTGGAGCGAGGCAGCAGCAGAGGGGGCAGTGGCAGCAGTAGTGAGGCCTGAGGACCCATCTGATGCCCAG GCTCTTCCAGCCACTGTCTAGACCTCCTCCATCAGATGCCTAGGCCTCCTCCATCAGATACCTGGGCCTC CTCCATCAGATGTCTGGGCCTCCTCCATCAGATGCCTAGGCCTCCTCCATCAGATGTCTGGGCCTCCTCC ATCAGATGCCTAGGCCTCCTCCATCAGATGCCTGGGCCTCCTCCATCAGATGTCTGGGCCTCCTCCATCA GATGTCTGGGCCTCCTCCATCAGATGCTTGGGCCTCCTCCATCAGATGCCTGGGCCTCCTCCATCAGATG TCTGGGCCTCCTCCATCAGATGCCTGGGCCTCCTCCATCAGATGCCTGGGCCTCCTCCATCAGATGTCTA GGCCTCCTCCATCAGATGCCTGGGCCTCCTCCATCAGATGCCTAGGCCTCCTCCATCAGATGTCTGGGCC TCCTCCATCAGATGCCTGGGCCTCCTCCATCAGATGCCTGGGCCTCCTCCATCAGATGTCTGGGCCTCCT CCATCAGATGCCTGGGCCTCCTCCATCAGATGTCTGGGCCTCCTCCATCAGATGCCTGGGCCTCCTCCAT CAGATGTCTGGGCCTCCTCCATCAGATGCCTGGGCCTCCTCCAGCTGGTGCCTGGGCCTCCTCCATCAGA TGCCTGGGCCTCCTCCATCAGATGTCTGGGCCTCCTCCAGCTGGTGCCTGGACATTCACCGTTTATACAT GGGCTCACACCCGTGTGAGCAGAGGAGACCCTGTGTTTTCAGTGGAATCAGATCTTCCTCAGTTGGGGCC TTCCTTGTCCCCCCGTGGACTCTCCAATGTGGGACCTCGGTCCTGGGCTCTCACCGGGGTCTGTCATTGG CTCTGCACAGGCTGCATGAACCCACGCACCTGTCCCTGACCTGCTGCTCTGGTCTCCTCACTAGCTCCAA TGTTCCCATGTTTCCATTTGTGTGTCTTATACGTGTATGTGTGCCCTCTCCTTACATACACACCCTGGAA TAGAGTTCTCACACCGTTCAATTTTGCACATATGTCCTCATGGATCCCCAGTTTTGTCCCTCCACTTGGG CACACGAGGTCTCAGGGGTCCTCAACCTGGGTCTGTGCCCCCAGCTGTGCCCAGCACCACGGGACCTCCA CAGTCCAATGGCATTGCCTGTGTGTGCTGTCATGAAGATACCACGGCTGTTCTGGTCAGCTCCTACCCTT ACATCTCTGTGTGTGTCGTCCCTCATCCCGTGTAAATATCACCATCCTTGTGACACTGGCACTAAGCAAT GTGTGCTCTGCCCAGCACACCTGGACATGTTCTCTCGGCTGGCTTTCTGTTCCCCGCCTGCCTCCAGGAG GCCAGGGTGGTGGTGCTGCTAGGCCATCCCAATGTTAGGCTTGTGGGTGATTCCCCGGGCCTTTTCATTC AAGCTGCACACAATGAGTCATTAAAGTTTGTCATAGAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6855024 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6855024 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12149 same embryo
	EMAGE:12147 same embryo
	EurExpress:euxassay_011116 same experiment
	MGI:4823635 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source