EMAGE mouse gene expression spatial database

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EMAGE:12236

Prokr1 prokineticin receptor 1 ( MGI:1929676)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008428. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008428_01	euxassay_008428_02	euxassay_008428_03	euxassay_008428_04

euxassay_008428_05	euxassay_008428_06	euxassay_008428_07	euxassay_008428_08	euxassay_008428_09

euxassay_008428_10	euxassay_008428_11	euxassay_008428_12	euxassay_008428_13	euxassay_008428_14

euxassay_008428_15	euxassay_008428_16	euxassay_008428_17	euxassay_008428_18	euxassay_008428_19

euxassay_008428_20	euxassay_008428_21	euxassay_008428_22	euxassay_008428_23	euxassay_008428_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12236_wholemount_strong.wlz

12236_wholemount_moderate.wlz

12236_wholemount_weak.wlz

12236_wholemount_possible.wlz

12236_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12236_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 13 14

telencephalon ventricular layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 23 moderate expression: see section 01

medulla oblongata alar plate ventricular layer

strong

regional

strong expression: see section 10 11 12 13 14 15

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 11 12 13

midbrain ventricular layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 18 19

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 17 18 19 20 21 22

spinal cord ventricular layer

strong

regional

strong expression: see section 12 13 14

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 11 17 18

neural retina

moderate

regional

moderate expression: see section 01 02 03 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9325
Entity Detected:	Prokr1, prokineticin receptor 1 ( MGI:1929676)
Sequence:	sense strand is shown >T9325 CTGCCACTCGAGCACGGAAAGACCCTCAGAAGACAGAGGTGCTCCTAGCCCTTGCTGCAGAGCTCCGGAG GAGCGTCTGAGATCCTCGCTCTGGTTCGCAGGTTGAAAATGGAACTCAGATACCGGGTCCCGCAGATGCT GGCTGGAAAGATCTGACTCACCGAGGGAGAGCCAAGTGCTTGCAGAGAGGAGTCGTCAACCTTGCGGATG CCAGTGGGACTGCTCCAGGGGTGTGCACCTGCCTAGATGGAGACCACTGTCGGGGCTCTGGGTGAGAATA CCACAGACACCTTCACCGACTTCTTTTCTGCACTCGATGGCCATGAAGCCCAAACCGGCTCGTTACCATT CACTTTCAGCTACGGTGACTATGACATGCCCCTGGATGAAGAGGAAGATGTGACCAATTCTCGGACTTTC TTTGCTGCCAAGATTGTCATTGGCATGGCTTTGGTGGGTATCATGCTAGTGTGTGGCATCGGCAACTTCA TCTTTATCACTGCCCTGGCCCGCTACAAAAAGCTCCGCAACCTCACCAACCTGCTTATCGCCAACCTGGC CATTTCAGACTTCCTCGTGGCCATCGTGTGCTGCCCCTTTGAGATGGACTACTATGTGGTGCGCCAGCTC TCCTGGGAGCATGGTCATGTCCTGTGCGCCTCTGTCAACTACTTGCGTACCGTCTCCCTCTACGTCTCCA CTAACGCCCTACTGGCCATTGCCATTGACAGGTATCTGGCCATTGTGCACCCGCTGAGACCGCGGATGAA GTGTCAAACAGCCGCCGGCCTGATCTTCCTGGTGTGGTCAGTATCCATCCTCATCGCCATTCCAGCTGCC TACTTCACCACTGAGACCGTGCTGGTCATCGTGGAGAGACAGGAGAAGATCTTCTGTGGTCAGATCTGGC CGGTGGATCAGCAGTTCTACTACAGGTCCTATTTCCTTTTGGTTTTCGGCCTCGAGTTCGTGGGCCCTGT AGTCGCCATGACCTTGTGCTATGCCAGGGTGTCCCGGGAGCTCTGGTTCAAGGCGGTGCCAGGCTTCCAG ACAGAGCAGATCCGCCGGAGGCTGCGCTGCCGCCGCAGGACTGTGCTGGGGCTCGTGTGCGTCCTCTCTG CCTATGTGCTGTGCTGGGCTCCCTTCTATGGCTTCACTATCGTGCGTGACTTCTTCCCCTCCGTGTTTGT GAAGGAGAAGCACTACCTCACCGCCTTCTATGTGGTGGAGTGCATCGCCATGAGCAACAGCATGATCAAT ACGCTCTGCTTTGTGACTGTCAGGAATAACACCAGTAAGTACCTCAAGAGGATCCTGCGGCTTCAGTGGA GGGCCTCTCCCAGCGGGAGCAAGGCCAGCGCTGACCTCGACCTCAGGACCACGGGAATACCTGCCACCGA GGAGGTGGACTGCATCCGACTGAAATAAGCAAAATGGTACCACAGCGCCCGGGTGCACACAGCAGCCATG AACTTGTTTTTCTGCGGAAGGCAGAGGAAAGAGACAACTACTTAGACACGCTATTCAAGGACCACTGAAG TGTGGAATTTCTGAAATGGAGACCTGAGATACTGTCACTAGTGGTCAGGGTTCACCGAATATCTAATTTT TGCAAAGACTAGACACCAGTGAAAGTCCTTTGACAAAGTAAAGTGGGGATTATAGCAAGGAAATATGGAT TGGAGTGTCTGGAAAAGGCTATTTCCAGAGAGAAGGCAGAGCAGGCTATGTAGGACTCTCCTGTCTGTGC TTAGGGTGGAGTACAGGGCTCTGTGTGGGCCTAGCTCACCTCTGGCAGGACTCTTGGGTTTTTGTTTATG TCTAGCATTAGCAAGCCCGGAGCAAGAGTATGGAAGAGGTTCCCAGATGTGCTGTAGTCCTTTTCTGCTT ACCTCAGAATCCCCACTGAGGGGAATCAGAAGCATGAGTGGACACTCCAGGACCCACGGCTAAGTTCTGC GCATCCACCCCCAACCTACGGTCAGATCTTAGGCCTAGGGCTCTGCACACAGACAGTGGGGGAGGGGACT TCTTTGGAGCACCGACTTGAGGGAGGAGCCACTTGGACTCTGGGTGCCATTTGCATAGGGAATTTGGGGT TCTGAGTCATAAGTTTGAGTTCAGGTGAGCATGATTCCCCCACTCATGGTCTACTCACTGCAGGGGAAAA GGAGGTGAAGAAGGGGAGGGTCAGGTGGGACCCCTGAAGGCATGCGTGCAGGTTAAAGGATGCTGTTGCT TCATCTCTCAGCATCTGTGCCCTGGACCACTCGTGCTTCTTGAGACTTCATGCCTCTGAGTTTATGGAGC ATAGACTCATGAAGGTAAACGTAGCACCTTGCTGTCCCGTTCCCACTAGCTCCCAAACACCGGTGTTTTC ATCTCAGGCAGCCTATTTGATGGGATTCCCTAACTGGTAAGACTGGGAGAAAGATTTCTTAACCAGTGGT ACCTGACTCTTGACCTGGTCATGGTCCTGGGAATGAAGACTTGCTGCTCTTTCCAAATGAAGGTGACCTT GGAGAAATCACCACATTTCTGCTCTTCAGCGCACATGAAGACTTGCACATCAGGGGTGCTGCCTGGACAC TCAGGAAACCGAAGATGACCAACAGTGAGCTCTAGGGACCCCACAGACTTATGAAAGTCTCATCCCATGT GATAACTCACCTTCTTAGCTTGGCATTTAGAATTCTGGCCTGAGAAAACCTGAGGCCTAGCTTTGACTAA GTCATCTACACAGACCTGAGATAAGACTTTCTAAAGATAAAGGTAATACTTAAGAAACCTTGTTAGAATT GTTGGGAGGTCAATCAAATAATCTGTGGAAGATAAGCTGTTAACTATCTGGATAGTAAAGTATTACCACC ATTTCCACCACCATCACCACCACCACCATCTCCAGCACCATCACCACCACCACCACCACCATCTCCACCA CCACCAGCAGCAACAGCAACAACAGTGAACTAGCAACTATGGAGTGACTGGACAAAGACAAACTTGTACA TATATTCTGTTTATGACTCTGTCAATCTAACCAACTTCTCTCAATGTTCTCCATGTGAATTATTTTCTCA TGCTTCTCAATACTGCATTGTGTCATCATAGCACATTGGTATCAATAAGACTGAGAATTTTCACGGCAGG TACATAAAGGAAGTGGTACCCAGTCTCCAGTCAGGACGTGAGCACCAGGCTCTGCAGTCTCAGGATCAGA GCATCCTTGTTGTCAGGTAGAAGCTGTTCCCACACATGCACCCAGAATCAGAGCATCCTCACTGGCCATC ATTCCGCACATGTCCCAGCCAGCATGAACAATGTCTCCCAGCTGCTTTTATTCTGAGTGTGCAGAGCAGC AGAGGGGCCGGGTCCCTCCCCTCAGATTTCTTTATAGGACAAGACATTAATTAAATTAACCTGTGTGTAT TTTCTTTCTGAATACAGGCAACTCTGTTTTTGACCACAACAATTTTTTTAGTTGTCTATTTCCCAGAAAA CACATTTCAAAGGACCATTTATGTATTCTTATTCAGCATGCAACAGGTTCACCTTACTTTAAGTCAGAAT TCTCTCAGAGAATAAAAATTCATTAATGTTCAAACCTAAATCAAACACAGAGAAACAAAACCCCTATTTT TTTATTTTAAACTTTATTACAAAAAATTGCAAAAAAAATAAAAATAAAAAAGGAAGAAAAGAAAATCACA TACCAAATTAACCAGACCAACAGACTCTTTTTTTTTTTTAATAGTAAAACCCCATATTGCATTAAAAATG GCTAAGTTAGTTTGGGGTGTAAAAATGCTTAGATGACAGGATTTATTGGGATCATGACACTGTTCCTCTT ACTAAAGCTAGATAAGAGTTTGTAGTTGCTAAATAGTTGTTGATTTTTTAAACTCATGACAAATTTTCTT CTGATTTTTCTGCTAAAATGTGTCAAAGTTTCACTTTCCTAAACTGTCTGTAATTCTTCCAGATATTGGT GTCTCCTTGTGATTTCACAGTGAATGCTTGTTATCAAATGGAATACTAGTAGATGTACACATACGAAAAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6848525 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6848525 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12233 same embryo
	EMAGE:12235 same embryo
	EMAGE:12232 same embryo
	EMAGE:12234 same embryo
	EurExpress:euxassay_008428 same experiment
	MGI:4827436 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source