EMAGE mouse gene expression spatial database

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EMAGE:12267

Kcna5 potassium voltage-gated channel, shaker-related subfamily, member 5 ( MGI:96662)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007013. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007013_01	euxassay_007013_02	euxassay_007013_03	euxassay_007013_04

euxassay_007013_05	euxassay_007013_06	euxassay_007013_07	euxassay_007013_08	euxassay_007013_09

euxassay_007013_10	euxassay_007013_11	euxassay_007013_12	euxassay_007013_13	euxassay_007013_14

euxassay_007013_15	euxassay_007013_16	euxassay_007013_17	euxassay_007013_18	euxassay_007013_19

euxassay_007013_20	euxassay_007013_21	euxassay_007013_22	euxassay_007013_23	euxassay_007013_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12267_wholemount_strong.wlz

12267_wholemount_moderate.wlz

12267_wholemount_weak.wlz

12267_wholemount_possible.wlz

12267_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12267_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vertebral axis musculature

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

pituitary gland

strong

regional

strong expression: see section 12 13 14 moderate expression: see section 11

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 12

diencephalon roof plate

strong

regional

strong expression: see section 11 13 14

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 08 09 10 14 15 16 17

pons mantle layer

moderate

regional

moderate expression: see section 07 08 16 17

midbrain ventricular layer

strong

regional

strong expression: see section 12 13

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 05 06 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 16 17 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 07 17 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 08 17 18 19 20

trigeminal v nerve

strong

regional

strong expression: see section 04 05 06 07 08 09 16 17 18 19 20 21

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 16 17 18 19

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 11 14 15 17 moderate expression: see section 07 08 16 18

vomeronasal organ

moderate

regional

moderate expression: see section 11

heart ventricle

strong

spotted

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16

esophagus

strong

spotted

strong expression: see section 12 13

tongue

strong

spotted

strong expression: see section 11 14

tongue muscle

moderate

regional

moderate expression: see section 11 12 13 14

stomach

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11

midgut

strong

spotted

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

kidney calyx

moderate

regional

moderate expression: see section 08 09 10 11 12 17 18 19 20 21 22

kidney pelvis

moderate

regional

moderate expression: see section 10 19

tail paraxial mesenchyme

moderate

regional

moderate expression: see section 15 18 19 20 weak expression: see section 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4335
Entity Detected:	Kcna5, potassium voltage-gated channel, shaker-related subfamily, member 5 ( MGI:96662)
Sequence:	sense strand is shown >T4335 GAGAGAGGCAGGGAGCAGGGGCAGCAGCTTCTTGACGTCAGGACCAGCCAAGGGGATCCAGCCAGCTACC CCAGCCAGCCTAGAGCCAGCGGGGGCCCTCGGCTGAAGAGGAAACCGCGAGGCGCGGAGCGTGAGTTGGG GTGTGGGAACCGGTTGTCTGGGGGCGCAGCATGCCCCTTGCCCCCGGGCCATGGAGATCTCCCTGGTGCC CATGGAGAACGGCAGTGCCATGACCCTCAGAGGAGGAGGGGAGGCAGGGGCAAGCTGTGTGCAGAGCCCC AGGGGAGAGTGTGGGTGCCCTCCGACGGCTGGACTCAATAATCAGTCCAAAGAAACATCACCGAGGAGGC GCGCCACACACGAGGATGCGGGCCAGGGTGGGAGGCCTTTGCCCCCTATGCCTCAGGAGCTGCCACAGCC TAGAAGGCCATCTGCTGAGGATGAGGAGGGAGAAGGCGACCCTGGCCTGGGCACAGTGGAGGAGGACCAG GCTCCTCAGGACTCAGGATCACTCCATCACCAGCGGGTCCTCATAAACATCTCGGGTTTGCGCTTTGAGA CGCAGCTGGGCACCCTGGCGCAGTTTCCCAACACCCTCCTGGGGGACCCAGTCAAACGCCTGCGCTACTT CGATCCCTTGAGAAATGAGTACTTCTTCGACCGCAACCGGCCTAGCTTCGATGGCATTTTGTACTACTAC CAGTCTGGGGGCCGCCTGCGCAGGCCGGTCAATGTCTCCCTGGATGTGTTTGCAGATGAGATCCGCTTTT ACCAGCTGGGGGACGAGGCCATGGAGCGCTTCCGGGAGGATGAAGGCTTCATCAAGGAAGAGGAGAAGCC CCTGCCCCGCAATGAGTTCCAGCGCCAGGTGTGGCTTATCTTCGAATACCCAGAAAGCTCTGGGTCAGCA AGAGCCATTGCCATCGTGTCGGTCTTGGTCATTCTCATCTCTATCATCACCTTCTGCTTGGAGACTCTGC CTGAGTTCAGGGATGAACGGGAGCTGTTACGCCACCCCCCAGTGCCGCCGCAGCCCCCAGCCCCTGCCCC AGGGGCAAATGGCAGTGGCTCTGGTGTCCTTTCCTCTGGCCCCACAGTGGCTCCGCTCCTGCCTAGGACA CTGGCTGACCCATTCTTCATCGTGGAGACCACATGTGTGATCTGGTTCACTTTTGAGCTGCTTGTGCGTT TCTTTGCTTGCCCCAGCAAGGCAGAATTCTCTCGGAATATTATGAACATCATTGATATTGTGGCCATCTT CCCCTACTTTATCACCCTGGGCACCGAGCTGGCAGAGCAACAACCAGGGGGTGGTGGTCAGAATGGGCAG CAGGCCATGTCCCTAGCCATCCTCAGGGTGATCCGCCTGGTCCGGGTGTTCCGAATCTTCAAGCTCTCCC GCCACTCCAAGGGACTGCAGATCCTGGGTAAGACCTTACAGGCGTCCATGCGGGAGCTCGGGCTGCTCAT CTTCTTCCTCTTCATCGGAGTCATCCTCTTCTCCAGCGCTGTCTACTTCGCAGAGGCAGACAATCAGGGG TCGCACTTCTCCAGTATCCCGGATGCATTCTGGTGGGCAGTAGTCACTATGACCACTGTAGGCTACGGGG ACATGAGGCCCATCACTGTAGGGGGCAAGATTGTGGGCTCACTGTGCGCCATAGCTGGGGTCCTCACCAT TGCTCTGCCTGTGCCCGTCATCGTCTCCAATTTTAATTACTTCTATCATCGGGAGACAGACCACGAGGAG CAGGCTGCCCTGAAGGAAGAGCAAGGCATCCAGAGGCGGGAGTCTGGGCTGGACACAGGAGGTCAACGGA AGGTCAGCTGCAGCAAGGCCTCCTTCTGCAAGACTGGGGGACCCCTGGAGAGTACTGACAGTATCAGAAG GGGTAGCTGTCCTCTAGAAAAGTGTCACCTAAAGGCCAAGAGCAACGTGGACCTGCGAAGGTCTCTGTAT GCCCTCTGTCTGGACACTAGCCGGGAAACAGATTTGTAAAGAGAGATCAAGGCAGACTTGCAACAGCAGA GCTGGGAACCGGTTCCTCTTGAGCTTGCTTATCTGCTTTACACCATAGAGTATTTAAGCCCACCTGGTCA CCCATCAAAGTCCGGATCCTGTCTTTCCCCGTTTACCCTTCCCACCCTCCTGCCCTCTAACTTCCCCATC CCCCCCCCTTTCCATAACACCAAGGGTCACCTATTTTTAAAAAGTATCGCATTCCATGACGCAGGAGCCG TTGAAGTGGTGAGCATTCACTGTAAGATGGATGTATTCATAGCCAGTTTTCTATACCCAGCAGAGGGATA ACTCAAACAAAATTGACTTTAAATAGCTCAGATCCCAAGAGAGTCCATAGCCCTCTCTATTCCTGTGTTC CAAATTTACTTTACACGTGATTATATTTGTATATGGCAAATATTATTCTTATGGCTGACGAGTGCATTTT GTACAGCAGTGCACATGAAGAATATTTGGGTGTGTTCTTAAGATGTACTTTGAATTATGAAGTCTTGGGG GGGGTCATCATTTCCTGGGACAACCCAGGGGCAGAGACACTAAAGAAGTTGTTCAAGTTTGTGTTCCCAA GCCTCCCATTATTCTGGGATGTACTATTGGTGCTTTGTGTCTAAGGTAGAGAATGTTCTAGAAGAGCAGC AAACCTGCTTTTTTTTTTTATTCCCCTTAAACATATCCTGTGACTTTCTTCACAGAAGCATTTTAAGGAT GTTGGAAGAAGGCTTTTGAGAATTCATTCTTTTCATTTTGATAGCAGGAAATAAAATGTGACTCTGTCGG GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4527103 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4527103 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12268 same embryo
	EMAGE:12269 same embryo
	EMAGE:12271 same embryo
	EMAGE:12270 same embryo
	EMAGE:12266 same embryo
	EurExpress:euxassay_007013 same experiment
	MGI:4825697 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source