EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12271

Wscd1 WSC domain containing 1 ( MGI:2448493)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007047. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007047_01	euxassay_007047_02	euxassay_007047_03	euxassay_007047_04

euxassay_007047_05	euxassay_007047_06	euxassay_007047_07	euxassay_007047_08	euxassay_007047_09

euxassay_007047_10	euxassay_007047_11	euxassay_007047_12	euxassay_007047_13	euxassay_007047_14

euxassay_007047_15	euxassay_007047_16	euxassay_007047_17	euxassay_007047_18	euxassay_007047_19

euxassay_007047_20	euxassay_007047_21	euxassay_007047_22	euxassay_007047_23	euxassay_007047_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12271_wholemount_strong.wlz

12271_wholemount_moderate.wlz

12271_wholemount_weak.wlz

12271_wholemount_possible.wlz

12271_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12271_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

moderate

spotted

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vertebral axis musculature

moderate

spotted

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

thymus primordium

strong

regional

strong expression: see section 11 12 13 14 15 16

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vagus x ganglion

strong

regional

strong expression: see section 08 18 19

optic ii nerve

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 15 16 17 18 19 20 21 22

spinal cord

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18 19

inner ear

strong

regional

strong expression: see section 04 05 06 07 08 09 17 18 19 20 21 22

retina

moderate

regional

moderate expression: see section 01 02 03 22 23 24

anterior naris

strong

regional

strong expression: see section 11 12 13 14 15 16

posterior naris

strong

regional

strong expression: see section 11 15

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 11 12 14 15 16 17 moderate expression: see section 08 09 10 18 19

vomeronasal organ

strong

regional

strong expression: see section 14

left ventricle cardiac muscle

strong

spotted

strong expression: see section 09 10 11 12 13 moderate expression: see section 06 07 08 14

right ventricle cardiac muscle

strong

spotted

strong expression: see section 12 13 moderate expression: see section 14 15 16

lower jaw incisor

moderate

regional

moderate expression: see section 11

lower jaw molar

strong

regional

strong expression: see section 06 07 19

upper jaw incisor

strong

regional

strong expression: see section 16 moderate expression: see section 11

upper jaw molar

strong

regional

strong expression: see section 05 06 07 19 20 21

urinary system

strong

spotted

strong expression: see section 06 23 24

kidney calyx

strong

regional

strong expression: see section 09 11 12 21 22 moderate expression: see section 10 18 19 20

kidney pelvis

moderate

regional

moderate expression: see section 20

ureter

moderate

regional

moderate expression: see section 11 19

male reproductive system

strong

regional

strong expression: see section 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8415
Entity Detected:	Wscd1, WSC domain containing 1 ( MGI:2448493)
Sequence:	sense strand is shown >T8415 GGTGTGAGCACCATGGTGCGTGCTAGAGCCGGCTAGGCTGCAGTGGTAGGCTGTGGCTGGGCGGGTGCAA CAGAGTGGTCAACGTCTTGTCTTGGCGGTGGCGAGTAGTGCGTGTCACCCGCGGTTCCTGAGACTGACGA GAGCCAGGAGAGCTAAGGAATGGGGAAAATGGTGACCCCTGGGCAGAAGCTACCAACAAGGGGCTTGTGG CTGCCGGAGAGAAGTGATGATGACATTTGAAACCCACAGGGACAGAAGCCCACAAGTGTGCTGACACCAC CGGTGTCAAGAGTCAGGATGCTGGGATGATGCCCTTGGAGGCCCTGGCGTCCCTCCTGCCCAGGTGCTAG GAGCCGTCCCCAGGCTCCAGCTTGGAGCCCTGCTGCCCAGGGGCATGGCCAAACCTTTCTTCAGACTCCA GAAGTTTCTCCGCAGAACACAGTTCCTGCTACTCTTCCTCACGGCCGCCTACCTGATGACCGGCAGCCTG CTGCTCCTGCAGCGGGCCAGAGTGGCCCTCCCACAGGCCCTCCGGGCTCCCGGCAGCCTGCAGGCCTTAC CAGTGGCCACCGTTGCACTGGGTGTGGGTCTACTGGACGGCAGGAGCCTTCGTGATCCCCACAGTAGTCC TGACCTACTACTTGATGTGGACACACTGAGGAGTCCTCTAGCCCGACTACCACCTGGAATCCGCTGGCCC CGGAGGAACCGCAGCTCGCTGCGGCGGCGTTGGCTCCACCACCTCACCAGTGACCCGCAGGGGCCTCCCA CCCTGAGTCCGGAGGCCTCTGGACCAGCCAACCATAATAGAGGCAATTACCTGGGATGCTTCAGTGAGGA GGGCCAGGAGAGAACTCTGAAGGGAGCAGTGTTTTACGACTTGAGAAAGATGACCGTGTCACACTGCCAG GATGCCTGTGCTGAGCGGTCTTATGTCTATGCTGGCTTGGAGGCTGGTGCAGAGTGCTACTGTGGGAATA GGCTGCCGGCCACACGTGTCAGCCTGAAGGAGTGCAATCAGGAGTGCAAGGGAGAGAAAGGCTCCATGTG CGGGGCTGTGCGCCGGCTCTCTGTGTACAGCGTGGGGCTGCAGCAGCCAGGCTCCAAGAAGCGGCGCACT GCCACCTACCGAGGGTGCTTCCCACTGCCTGAGAATGTCACCCACACCTTCTCCTCCTCCATGACACAGG CCAACATGACTGTGGAGACGTGTTCTGGATTTTGTTCCCAGAAAGAGTTTCCTCTGGCCATCCTCCGAGG CTGGGATTGCTATTGTGCCTACCCTACCCCCCAGTTCAGCCTGCGGGATGCTGTGGATGGAGCACTGTGC AGTCAGGCCCCAGAGACACAAGGGCTACCTGGCTACTGTGAGGTCTACCAGACACCCGTGCAAGACACCC GGTGCACAGACAGGAAGTTCCTGCCCGACAAGTCTAAGGTGTTTGTGGCTCTGTCGAGCTTCCCTGGAGC TGGGAACACATGGGCAAGGCACCTGATCGAGCACGCCACTGGCTTCTACACGGGAAGCTACTACTTCGAC GGAACCCTGTACAACAAGGGGTTCAAGGGTGAGAAGGACCATTGGCGGAGCCGGCGCACCATCTGTGTGA AGACGCACGAGAGTGGCCGGAGGGAGATTGAGATGTTTGACTCGGCCATCCTGCTGATCCGGAACCCCTA CCGGTCCCTAGTTGCTGAATTCAACAGGAAATGCGCTGGACACTTGGGCTATGCCCCTGACCGCAATTGG AAGAGCAAAGAATGGCCGGAGTTTGTGAATAGCTATGCCTCCTGGTGGTCCTCACATGTCCTAGACTGGC TCAAGTATGGGAAGCGGCTGCTGGTCGTGCACTATGAGGAGCTGCGGCACAGCCTGGTGCCCACACTTCG GGAGATGGTGGCTTTCCTCAATGTGTCCGTGAGCGAGGAGCGGCTTCTCTGTGTGGAGAACAACAAAGAG GGCAGCTTCAGGAGGCGTGGCCGGCGCCCTCATGACCAGGAGCCCTTCACCCCTGAGATGAAGGACTTGA TCAATGGCTATATCAGGACAGTGGACCAGGCCTTGCGTGACCACAACTGGGCTGGGCTGCCCAGAGAGTA TGTGCCCAGATGATAGGCCCACCCTGATTTCCTGCTCCTGCTGAGATGCGGTTATACCAGTGGGCTGAAC ACTGCTCTAGTGCATTGATCCATGGACCCCTGGGAAGCATGGGGGCTGGCACACAGGCAGCAGGGTGTTC TTGAAGGGGCTGCTTTGTGTGCAGGAGATCTGAGGCAGCACAAGGCTTACCCAGAGGGATGTGTATCACA GGACACTCCTTGCCGGACACTTACGCATCCTCAGACACACTTAGACATCACCCCATTCTCACAGTGACCA TTTTAATGTGCCTAAGCCACTTGAGTGGGGTGGGTCAGACACTCCCAGCTTTGCACACACAGACAAATGG ACACACACACACACACACACACACACACAATGTGCCAGAGACTGACTATCTGCCTCACACACGCTCCAGG CTTTGGGAGACCCTGGGTTTCCTGGGCTCAATTGGGCACAAATTGGCTTTTGGGGGCCCTTGGCTCTGGG ATATTGTGCTGGGGAGACACTATTGATCTGAGTAGTGCACTGAGGACTGGAGGTACATAGACAGACTGTT GCTGATGGGTACTGTATGACCCTGACTGCTTGTCTGACATCCCATAAATGTGTGTGCTGTGACATCCTGG CACTGCAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5687975 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5687975 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12268 same embryo
	EMAGE:12267 same embryo
	EMAGE:12269 same embryo
	EMAGE:12270 same embryo
	EMAGE:12266 same embryo
	EurExpress:euxassay_007047 same experiment
	MGI:4829232 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source