EMAGE mouse gene expression spatial database

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EMAGE:12312

Arhgap39 Rho GTPase activating protein 39 ( MGI:107858)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010956. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010956_01	euxassay_010956_02	euxassay_010956_03	euxassay_010956_04

euxassay_010956_05	euxassay_010956_06	euxassay_010956_07	euxassay_010956_08	euxassay_010956_09

euxassay_010956_10	euxassay_010956_11	euxassay_010956_12	euxassay_010956_13	euxassay_010956_14

euxassay_010956_15	euxassay_010956_16	euxassay_010956_17	euxassay_010956_18	euxassay_010956_19

euxassay_010956_20	euxassay_010956_21	euxassay_010956_22	euxassay_010956_23	euxassay_010956_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12312_wholemount_strong.wlz

12312_wholemount_moderate.wlz

12312_wholemount_weak.wlz

12312_wholemount_possible.wlz

12312_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12312_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

weak

regional

weak expression: see section 04 05 22 23 24

upper leg muscle

weak

regional

weak expression: see section 04 05 06 07 08 20 21 22

diaphragm

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

lower leg rest of mesenchyme

weak

regional

weak expression: see section 04

vertebral axis musculature

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

brain

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 17 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 07

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 17 18 19 20

spinal cord

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 11 16 17

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 13 15 16 17 weak expression: see section 09 10 11 12 18 19

vomeronasal organ

moderate

regional

moderate expression: see section 13 16

tongue muscle

weak

regional

weak expression: see section 12 13 14 15 16 17

stomach

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11

midgut

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16 17 18 19

metanephros

weak

regional

weak expression: see section 07 08 09 10 11 18 19 20 21

tail paraxial mesenchyme

weak

regional

weak expression: see section 10 11 12 13 14 15 16 17 18 19

tail dorsal root ganglion

moderate

regional

moderate expression: see section 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9337
Entity Detected:	Arhgap39, Rho GTPase activating protein 39 ( MGI:107858)
Sequence:	sense strand is shown >T9337 GGGGGACGCGCGGCCGCAGCGCACTGGACCAGCGGCGGAGACCCAGGACGTGAAGCGCTGGCTGCGGCGG CGACGGCGGCCGGGAGCCGCCGGGAGCTGGGGCGCAGCGTGCGGTCGGTGATGAGGGGCAGTGGCGGCGC TCCGGGCGCAGGGCCGGCTGTGTGGCCTCTTCCTGTGTGATGCTGGAGGAGGCAACCTGATGATCCTCTG ACAACTGTTCACGTCAGCACTAAGGAAGCAGATGGCAATGTCACAGGCACAGGATTATGAATGCAGAAGC CATCATGTCGATGAGCAGGAGCCCAGGATTCCGGGGTCGAGTACCAGGTTGGAGTGGGTGGAGATTATCG AACCACGCACGCGCGAACGTATGTATGCCAACCTGGTCACCGGAGAGTGCGTGTGGGATCCACCAGCCGG TGTGCGCATCAAGCGCACCAGCGAGGACCAGTGGTGGGAGCTCTTTGACCCCAATACATCACGCTTCTAC TACTACAGTGCTGCTTCCCAGCGCACCGTGTGGCATCGCCCACAGAACTGCGACATCATTCCTCTGGCCA AACTGCAGACGCTGAAACAGAACACTGAGTCTCCTCGTGCCTCTGCGGACAACAGCCCAGGGAGGGGCAG CCGTGATGGTAGCACTGGCTCCTCGCTGGAGCCAGAGCTGGAAGAAAGGACACAGGAGCTGCCAGTTCGC AGTGGGCGGGCAACAACCTTGGTCACGTCAAAGGAGGATACTAGCAGTTGTTCGCCACCAGGTGTGCTCC TGGAGAAGGACTATGAGGTATACCGGGATTACAGTGCCGACGGCCAGCTCCTTCACTACAGGACCTCCTC ACTGCGATGGAACTCTGGTAACAAGGAACGCATGCTCATCAAGGTTGCCGACCGTGAACCCAGCTTCCTC ACTCCCCAGGGCAATGGCTATCCCGCAGACAACCAACCAGGGGGCCATCACCGCAGACCATCTGGCAGCC AGCACTCACCTAACCTGCAGACCTTTGTCCCTGACACTGATGGCACTGTCTTTTTTCCAGAGAGGCGGCC CTCACCCTTCCTAAGGAGGGCTGAGCTCTCTGGGAACTGTTCTCCACTGCTGATACAGCCCCGGAAACCC TCCAGTGACTCACAGCCCTCCTCCCCTCGCTATGGTTATGAACCCCCACTCTATGAGGAGCCTCCAGTGG AATACCAGGCCCCTATTTATGATGAGCCCCCCATGGACGTGCAGTTTGAAGCCAACAGCCCTTACCAGAC AGGCTCACCACAGAGGTCCCCAGGCCGAAAGCCCCATCCATTCCTGCAAACTACCAAACAGACCCCAACA TCACCCTGCCAGCAGCTGATGCGCACCAAGCAAAAGTGCCCTGAGCGTTTTCTGAGCCTGGAATATAGTC CTGTGGGCAAGGAGTACGTGCGGCAGCTGGTGTATGTGGAGCAAGCAGGCTCCAGCCCCAAGCTTAGAGC TGGGCCCAGGCACAAGTATGCACCCAACCCAGGCGGGGGCACCTACTCCCTGCAGCCCAGCCCCTGCCTG CTGAGAGACCAGCGCCTGGGGGTCAGGTCAGGAGACTACAGCACCATGGAAGGGCCCGAGTCACGCCCAA GCCAACCACCCACACCACTGCCCCAAGCCCAGGAGGATGCCATGTCCTGGTCCAGTCAGCAGGACACCAT GTCTTCCACAGGCTACTCCCCGGGCACTCGCAAGAGAAAGAACAGAAAACCCTCTCTGTGCCAAGTCCCT AGCACCTCGTCCACTGATGGTGCTGGGGGCCTGCTAGGAGAACAGCCTCTGACTGAAGAGCGCTCTCCAT GCAGGGCCAGCCTTACCCCAGTAAAGGCTGAGGCCGACCTAGTCCGAGGCACACCTGAGCCCTTCCTGGC ACAGGCCCGGTTAGCCTGGGAGGCACAGCAAGCCCACTTCCACATGAAACAGAGGGGCAGCTGGGACTCC CAGCAAGATGGCTCAGGGTATGAGAGTGATGGCGCTGTCCCACTGCCCATGCCTGGGCCTGTGGTGCGAG CATTCAGTGAGGATGAGGCGCTGGCGCAGCAGGACAGCAAACACTGGAAGCGGAGCACCTTTGACAAGCT TGGCTTCCCTCAGATCCTGCTGGAGAAAAGCGTCTCTGTGCAGACCAACCTGGCCTCACCGGAGCCCCAC CTCCACCCCTCACAGTCTGAGGACCTTGGTGCCTGCGCCCAGTTTGAGAGCAGCCGACAGAACCGAAGCG CAATGCCCAGCTCCAGCTGTGTCTTCCCCACATTCACGCTGCGGAAGCCCTCCTCAGAGACGGACATCGA GAACTGGGCCTCCAAGCACTTCAACAAGCACACCCAGGGGCTCTTCCGGCGCAAGGTGTCCATAGCTAAC ATGTTGGCCTGGAGCAGTGAGTCCATCAAGAAGCCCATGATTGTGACTAGTGACCGGCACGTGAAGAAGG AGGCCTGCGAGATCTTCAAGCTAATCCAAATGTATATGGGGGACAGGCGCGCCAAGGCAGACCCACTGCA CGTAGCCCTAGAGATAGCCACCAAAGGCTGGAGTGCACAGGGCCTGCGGGATGAGCTGTATATCCAGCTG TGCCGGCAAACCACAGAGAACTTCCGCCTAGAGAGCCTGGCCCGTGGCTGGGAGCTCATGGCCATCTGCC TGGCTTTCTTCCCACCAACCCCCAAGTTCCACTCCTACCTAGAGGGCTATATCTACCGGCATATGGACCC TGTTAACGACACCAAAGGGGTGGCAATAAGCACCTATGCGAAGTACTGTTACCACAAACTGCAGAAGGCA GCTTTGACTGGGGCCAAGAAGGGCCTGAAGAAGCCTAATGTGGAGGAGATCCGACATGCCAAGAACGCAG TGTTCAGCCCATCCATGTTTGGCAGTGCACTGCAGGAGGTGATGAGCATGCAGAAAGAGCGCTATCCTGA CAGGCAGCTGCCCTGGGTACAGACACGGCTCTCCGAGGAGGTCCTGGCACTCAACGGTGACCAGACAGAG GGTATCTTCAGGGTCCCTGGGGACATTGATGAGGTGAATGCCCTCAAGTTGCAGGTGGATCAGTGGAAGG TGCCTACAGGCCTGGAGGATCCCCATGTCCCTGCATCGCTGCTGAAGCTGTGGTATCGGGAGCTGGAAGA GCCCTTGATTCCTCATGAATTCTACGAACAATGCATTGCGCACTACGAGAGCCCGGAGGCTGCTGTGGCC GTGGTGCACGCGCTACCGCGCATCAACCAAATGGTGCTGTGCTACCTCATTCGCTTCCTCCAGGTGTTCG TGCAGCCAGCCAACGTAGCCATCACCAAAATGGACGTCAGCAACCTGGCTATGGTGATGGCACCCAACTG CCTGCGCTGCCAGTCGGATGATCCTCGCGTCATCTTCGAGAATACACGAAAGGAGATGTCCTTCCTTCGA GTGCTCATTCAGCATTTAGATACCAGCTTCATGGAGGGCGTGCTATAGCACGCGGGCTATGGACGCCTGC TAGGGTGACACCCGGGAAGGTGGGAGGGAAGGCCCCAGCGCGAGGCAGCATGCCAGGCCCCGCCAGACGC CGGCCCCGCCCTAGGCCCTCCCGGGGCCAAGCAGCCGCGCCATATCTCGCGGCCAGTTCCTGCAGGTACC GGCTCGCCCTGCCAGTCGCAAGCCAGCCCCGAGAAGTGCCTTCTGTTTCCCGGAGCCGAGCGACACTGCC CTCCTGGTCGGCTGAAGCACATACCGCGTTGCTCAGGGCAACAGGACCTTGTCCCCTCTTCAGGGCAATC CTCCACCAAGGCACTCTCGCCCTGATCCCTCCCACCCTAGTCTAGGGGCCTCTGGCTGGGGCCAGAAGGA GAGTTAGAGGCGTTCTGGAGAGCTGTGCTGGCCACTACTAGGACTTGGCTCCGTCGGCTCGATGCCTAAT AGGTCTTTGTGCAAGGGGAGGCCCCAAACCAAAGCCCTCTGGGTTAGGGCCAGGGCTAGGCAGGCTTTCT TGGGGCGGGTGAGGGAGGGCGAGAGGTTTTTTGTTTTTTGACTACAGAATCTATCCGATACCCAGCCTAC TTGTGTATAAAGATATAAATAGAACGACAGAACTAAGTTTGCTAAGGACATAGTTTTAACCAGATGCTAT TTAGCTCTTGAACTGCCATTGCGGTCCTCGCGCTGAGCAAGTTAGCACCACCCTCCTTTGACCTCGCGGC CTCTGACCAAACTCCACACACGGGCGGCGCGTGGATGAGACCTGCCTGGACTCGTTTGCGTTTTCCATTC TGGAATCTGAGTTCCAACAGTTGTCAAACTTCATTTCTCCTGTTTATATATATATATTTTTAGAGTTTTT ATTTATTAAAAACAAAAGTCCCGTCATGCGGAGGTCCTGGCCTCTCTCTCCAGGGCCTCTCGGCCGCAGG CGCTCCCTCGTACGTGTGGCTCGAGGGGACAGTGCTCCGTGTGAATAAAGTTCAAGTGCACACTCAAAAA AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6849400 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6849400 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12311 same embryo
	EMAGE:12314 same embryo
	EMAGE:12313 same embryo
	EurExpress:euxassay_010956 same experiment
	MGI:4824197 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source