EMAGE mouse gene expression spatial database

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EMAGE:12321

Ppp1r3b protein phosphatase 1, regulatory (inhibitor) subunit 3B ( MGI:2177268)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011061. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011061_01	euxassay_011061_02	euxassay_011061_03	euxassay_011061_04

euxassay_011061_05	euxassay_011061_06	euxassay_011061_07	euxassay_011061_08	euxassay_011061_09

euxassay_011061_10	euxassay_011061_11	euxassay_011061_12	euxassay_011061_13	euxassay_011061_14

euxassay_011061_15	euxassay_011061_16	euxassay_011061_17	euxassay_011061_18	euxassay_011061_19

euxassay_011061_20	euxassay_011061_21	euxassay_011061_22	euxassay_011061_23	euxassay_011061_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12321_wholemount_strong.wlz

12321_wholemount_moderate.wlz

12321_wholemount_weak.wlz

12321_wholemount_possible.wlz

12321_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12321_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foot mesenchyme

moderate

regional

moderate expression: see section 04 05 06 07 22 23 24

lower leg rest of mesenchyme

moderate

regional

moderate expression: see section 02 03 04

head mesenchyme

moderate

regional

moderate expression: see section 24 weak expression: see section 01 02 03 23

adrenal cortex

moderate

regional

moderate expression: see section 08 09 10 18 19

cochlea

moderate

regional

moderate expression: see section 03 04 05 06 07 08 15 16 17

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 21 22 23

cornea

moderate

regional

moderate expression: see section 03 04 23 24 weak expression: see section 01 02

nasal septum

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17

viscerocranium

moderate

regional

Expression in the turbinate bone.

esophagus

strong

regional

strong expression: see section 11 12

stomach

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09

mandible

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 15 16 17 19 20 21 22

lower jaw incisor

moderate

regional

moderate expression: see section 16 17

maxilla

moderate

regional

moderate expression: see section 05 06 07 17 19 20 21 22

bladder

weak

regional

weak expression: see section 13 14 15

renal cortex

moderate

regional

moderate expression: see section 07 08 09 10 11 17 18 19 20 21

urethra of male

strong

regional

strong expression: see section 14 15

larynx

weak

regional

weak expression: see section 11 12

lung

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

nucleus pulposus

moderate

regional

moderate expression: see section 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9413
Entity Detected:	Ppp1r3b, protein phosphatase 1, regulatory (inhibitor) subunit 3B ( MGI:2177268)
Sequence:	sense strand is shown >T9413 GGAAAGTTAAAGTGAGCCACAGATTGCTGGAGCTGGAATCCAGAGTGCTGCAATCCAGTGAGCAGACTGC TAGCCGGCTCGCCTAGCGTGATGGCTGTGGACATCCAATACAGCTACAGCAGTATGGCCCCTTCTTTACG CAGAGAGCGCTTCACCTTCAAGATCTCCCCCAAACTGAGCAAGCCCCTGAGGCCTTGTATCCAGCTGGGC AGCAAGGATGAAGCCAGTGGAATGGTGGCCCCCGCAGTGCAGGAGAAGAAGGTGAAGAAGCGGGTGTCCT TCGCCGACAACCAGGGGCTGGCCCTGACAATGGTGAAAGTGTTCTCGGAATTCGATGACCCACTAGATAT TCCCTTTAACATCACCGAGCTCCTAGACAACATCGTGAGTCTGACGACAGCAGAGAGCGAGAGCTTTGTT TTGGATTTTCCGCAGCCTTCCGCAGATTACTTAGACTTCAGAAATCGCCTTCAGACCAACCACGTCTGCC TAGAAAACTGTGTGCTGAAGGATAAGGCCATCGCGGGCACCGTGAAGGTCCAGAACCTGGCCTTCGAGAA GGTTGTGAAGATCAGGATGACATTTGACACCTGGAAAAGCTTCACAGATTTCCCTTGTCAGTATGTGAAG GACACTTACGCTGGTTCAGACAGGGACACATTCTCCTTTGACATCAGCTTACCCGAGAAAATTCAGTCTT ACGAAAGAATGGAGTTTGCCGTGTGCTACGAGTGTAACGGCCAGGCGTACTGGGACAGCAACAAAGGCAA AAACTACAGGATCACCCGGGCTGAACTCCGATCCTCTCCGGGAAAGATTGAGCCGTACAATGGACCAGAT TTTGGAATCTCTTTTGACCAGTTTGGGAGCCCTCGGTGTTCCTTTGGCCTGTTTCCAGAGTGGCCTAGTT ATCTGGGATATGAAAAGCTGGGGCCCTACTACTAGTGACTGCAGGTGACAGTCCGTGGCTCAGATCAAGA TGGAGGCGAGAGAACGGAAGGCCAGAGGAAAGTTGAACTGCCACTAGGCGTGGTTTTTGAAAAGACAGGC TCCTGTTCACCTTTTTCTTAAACCAGCAAGTCCAGCGTGTTTCCATCACACCCGGTTTCTTGCTCAGAGG AGTGGAGCGGTGCTGATCCTCTGTGGCATCCGGGTTGGCCACAAGGGAATGCGCAGGATGGTGCCAGAAG GGTCTGGAGAGGAGCCAAGCTGGCAAGGACAGAGGAAGCTGGCCTCTCTTCCACACTTGGACTCTGAAAG TCACCCAGAAACCAATCTCCAGCCTTACCATTGACCTCGAGGTCCTGTCCCCGCTCATGGCTTTCCATGC GTCTCCTTCATTCCACGAGTACTCCGAAGGGAGCCACCTCAGTTCTGGGACACACTGGCCTGATTGATAC CCAGCAGCCTCAGAAGAGGGCCGGGGGCGGGCGCTTTCTGTGCGAGCTCTTTCACTACACTTCCTCCCGC TACAGTCTATCCAGTGATCTGGACAAGAGAGACAAAGCAAATAAGTAGCTCGTGTCTGGGATAGGCATTC CCTCCCCCCTCTCTCCTCCTGACCCCTCCTTTATATTCCAGAAGAGAGCAACATATTAGTTACTAGAAAG TCATTGGTTCCCATGTGGGGTCACAGGGTACAGCACTGGGACAAGGTGGGACAGTCTGAACTTTTAGCTT TTCCTCTTGGATGTTGGGGGAGCTTTTGAAGCACCGTTTCACCCGGAGTTACCAGAAGAGGACTCAATGA CAAGATGCATTTCCACTTGCCTGGCCGGTGTGTGTGAACTGTTTGTGTTTGAAGAGAGTCATAGCTCTCA TGTCTCCTGGAGCCTCCACTTCAGTGCCTTGTCTGAGGCTTTCAAATAGCAAGACTGGCATCTCCTCGAG TCCTCCAGACTGTGCTTAGATATATATTCATATTCAGGATGCTTTATGAACCCCCAAGGACATTGATCGG GTGTTTTTATTTATGGTGTGTATTATAAAAAGATAGGGGAGAAAAACCTCACGTTTTTCTATGTTATTTT AAATGTTTGTCAACTCTGAGGTATCAGATGAAGTTTTCTTAACCATATTGGAGTGCAATGCGCTTTCATA CGCTAGCGTGAGACTCCTGGAGTTCTTACCCTTGGGTGAACCCCCAAACCCTGTGATTTTTCTCTAGGGC AAATTAGAATTATAGGGGAGATTTTTTTAAATGCGAGTTTGTAGAATTAGGGGTGTTGTTTTGTTTTGGA AGTTTGATAAAAGATGCCTGTTTCATTTCCTTACTGAAGTGATGTTTCCCTGAGACAAGACAGAAAGGAG CAGAGTCTCATGGTTTCGGAGGTGGGGGCCACTGGACGTAGCCCTGTGCCCTCTGGCTTTATAAGTTAGC ATCAGGCAGACATCAAATGTTTTTAATCCTAGCATGTGTGGTTTATTTTTTATCTTAACTTTTTCACTTT TGAAGATTCAAATGTTTTGACTTTTTAAATGTTATTTTCTATGATTTCTTATTCCTAAAACTGGTACTAG TCTGTAATCTGTGCTTTGTATATTGTGCCTCTCCCAGATGGGTGGCGATCTGGGATTGCATGCTGATTAT CTGTGAGGAAATCATCTTTGTGCCATGAGGCTGAAGCTGGGGCCAGGAGTTTGTGTTTTATCTAAGCAGG CCATGTATCTCCTAGATGGGTGTACTTTAAATGTTCCGGATTTGTTGACCAGAGCAGCTAGGTTGAAATT GGATAGAAAGCAAAACTGCTCTCTATGGGTCATGCATTGTGTGTTAAGAAGGAAGGCAGCCAAGGGCACT GTTTCTTACATAGATGCAGACTAAAGATCATAAAATGGCTGGGGGCGGGGGCGAGGTACGGAATGGAATC TGTTCAGTGGTATGGATGATCTTCAGACATTCCGAGTCAAAATATAAAATATGGTAATTCCATGTTATAT GTCCCTCCTAAAGCAGACATACCCTCACCCCCTTTTGCCCAGATTTGCCCCAGCTTTCTTGCTCACGAGC ACTGCTTGAAAGCCCCCCAGCCAGCACACCTGGGGTGCACACCCACTGCTCAGCTCCAGGGGCATGTTGT GCCTTTGCCTGCTCACCAGGACCCTTCCCTTGAAGCAGTCCGTGGGCTGTGCTTGACTTCCTTATCAAGG CTGTCACTCAGAGACAGAAGAGTCTTCCTTCTGCTTTCTGCCTCCAATACAGCCCTCCTTCTTTTGACCC AGTCTGTCCCATGACTCTGAGATAGCCTCGGGAGCTGCATTTAGAAACTCTATGCATTGTTCAGAGTTCA CCACTGTAGACAAATTTACGCAGCTCAGAAGACGACAGGCCCCTTGCTTGAAGATCCCACGGCAAGTTTG ACCTGGAGGTCTGTCTTCATGCAGTTCTTGGCTAGGATGATGGATGGATCCTAATCCCCAGACATTGTGA TCCAGGAAGGGAAGGCAACCCCATTCTTTGTTCCATATTTATTTCCCCAAATTCCCTGGGGTGCTTTGGC AAAATGGGTACAATAAATAAGCTAGACCATAAGACATGTTTCTTGTAAGAAGCCGTTTGAGTGTTGTAAG AATATTTTCTCTAGAAAGCTAGGAAGGCATAATGGAAGTCTGTAGTTCTGCAGAAACTAAAGCAACTTGA GTCAATGGTGGGAGTTATTTTATCCCTGAGATGTAAGCTTGGTGGCTCCTGATTCTGGGAAATCTGCACT CAGCCGCATCTCCTGGCATGATCACAGAAACTGCAGATAAGATGTTCCCGAAATTCTGGGATCTTACCTC TAACGGGATCGTCCAGAGTCACTCAAAGACATTTCTCTTGCTGAGGTTCTGTGAGATGCCTCCAGCTCCA TCAGCAAAGCTGTGAGTGTTTCCACGGTTTTGCTGCAGTTCCCAGAGGCCCCTGGGGCACGTGGACTTCT AGAGTGCACACGTGATGATGTTACACTTTTGCTTTAATGTGAACATTCAACTGACGGGCTTGTCGTCATT GTATGCCTACATGTGCCTTGTTCAGAAACTTCTGCATGTTTGTAAGACATATTTTTGTATACATTGGTAG AGGGGAAATGCACTTTAAAAAAAATCACAAGAATGGATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT GTGTGTGTGTGTGTGTGTGTGTGTGGTGGAAGAGAATGATTTCTTTGTATCTCAAAAGCAAAAACGTAAT AAATATAGAAAGAAAAGAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30357193 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30357193 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12322 same embryo
	EMAGE:12323 same embryo
	EurExpress:euxassay_011061 same experiment
	MGI:4827376 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source