EMAGE mouse gene expression spatial database

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EMAGE:12336

Ppm1l protein phosphatase 1 (formerly 2C)-like ( MGI:2139740)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008476. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008476_01	euxassay_008476_02	euxassay_008476_03	euxassay_008476_04

euxassay_008476_05	euxassay_008476_06	euxassay_008476_07	euxassay_008476_08	euxassay_008476_09

euxassay_008476_10	euxassay_008476_11	euxassay_008476_12	euxassay_008476_13	euxassay_008476_14

euxassay_008476_15	euxassay_008476_16	euxassay_008476_17	euxassay_008476_18	euxassay_008476_19

euxassay_008476_20	euxassay_008476_21	euxassay_008476_22	euxassay_008476_23	euxassay_008476_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12336_wholemount_strong.wlz

12336_wholemount_moderate.wlz

12336_wholemount_weak.wlz

12336_wholemount_possible.wlz

12336_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12336_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

strong

regional

strong expression: see section 01 02 03 04 05 22 24

upper leg muscle

strong

regional

strong expression: see section 01 02 03 18 19 20 21 22 23 24

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

forearm rest of mesenchyme

strong

regional

strong expression: see section 01 02

hand

strong

regional

strong expression: see section 01 02 03 22 24

foot

strong

regional

strong expression: see section 01 02 03 16 17 18 19 20 21

lower leg rest of mesenchyme

strong

regional

strong expression: see section 01 02 20 21 22 23 24

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

thymus primordium

strong

regional

strong expression: see section 11 12 13 14 15

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

strong

regional

strong expression: see section 04 05 19 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 18 19

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 17 18 19 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 08 18

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 06 07 18 19 21

trigeminal v nerve

strong

regional

strong expression: see section 09 10 17

spinal cord

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17

cervico-thoracic ganglion

strong

regional

strong expression: see section 10 16 17

cervical ganglion

strong

regional

strong expression: see section 09 10 14 15 16 17

thoracic ganglion

strong

regional

strong expression: see section 12 13

dorsal root ganglion

strong

regional

strong expression: see section 06 07 09 10 11 12 17 18 19 20

cornea

strong

regional

strong expression: see section 03 04 23

eye skeletal muscle

strong

regional

strong expression: see section 04 05 06 07 20 21 22

neural retina

strong

regional

strong expression: see section 01 02 03 23 24

anterior naris

moderate

regional

moderate expression: see section 13

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 15 16 17 18 19

vomeronasal organ

moderate

regional

moderate expression: see section 12 16

esophagus

strong

regional

strong expression: see section 13

tongue muscle

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17

stomach

strong

regional

strong expression: see section 03 04 05 06 07 08 09 moderate expression: see section 01 02

midgut

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15

liver

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 17 18 19 20 moderate expression: see section 15 16 21 22 23 24

left lung

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12

right lung

strong

regional

strong expression: see section 13 14 15 16 17 18 19 20 21 22 23 24

trachea

strong

regional

strong expression: see section 13 14

tail paraxial mesenchyme

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9259
Entity Detected:	Ppm1l, protein phosphatase 1 (formerly 2C)-like ( MGI:2139740)
Sequence:	sense strand is shown >T9259 GGAGAGGCAGGCAGAGCCTGCCGGCGGCTCCGCACCCATCGTGCTGCGTTTCCCAGCTCGGTGTCCCCCT GCCTTTGCCCAGGCACCGGCAAAACTTTTATTGTTTCGGGGACCCCCCCTGGATGTGTGACGAAATCGCA ACGCTCCGCTGTCTCGTCCCCCAAGCTCGCCTGCAGCGGCGATCGAGGGAACGTGCAACCCTCGCCTCCG GGCTCTTGCGCCCTGCGCTTCCTCACCTGCCGATCGCTCCCGGGAACCGCGAGGGGGGACCCCTAAAGGG ACCGCGCGGGCATCGGCACCCCGAAATCCTCTTCACCGTTGCGGACCGGCTCGGCGCAACTTTAACTTGA TTCCTGTCGCCCACCCGGGGCACCCGCGGCGGCCGCCGCCGCCGCCGTCGCTGCGGTCTCACAAAGAGCG CGGGGAGGGAAGGCGGCGCAGGCGGCAGGCACGGGCCTTCGGGCTGCCCAGACAAAAGGGAAACTGCCAT CGTTCTGGACGACTGTTCCGAGCGGCCGTGGGTTCGTTCACCCTGTCGGCCCGGGCTGCCTCTCCGCCTT GGTCCCCGCGGGCAGTGCCCGCAGTACTCGGACCCCGCGAGTTGCGGGGCACGCGTCGCTGCTGCTGGTC CGGGCTCTAGGGATAATAAATGATAGAGGATACAATGACTTTGCTGTCTCTGCTGGGTCGCATCATGCGC TACTTCTTACTGAGACCCGAGACGCTTTTCCTGCTGTGCATCAGCTTGGCTCTCTGGAGTTACTTCTTCC ACACGGACGAAGTGAAGACCATCGTTAAGTCTAGCCGGGACGCGGTGAAGATGGTGAAGGGCAAGGTGGC CGAGATTATGCAGAACGATCGTCTTGGAGGACTTGATGTGCTGGAGGCCGAGTTTTCCAAGACCTGGGAA TTCAAGAGCCATAATGTGGCGGTGTACTCCATCCAGGGCCGGAGAGACCACATGGAAGATCGCTTCGAAG TTCTCACCGATCTAGCCAACAAGACGCACCCGTCCATCTTCGGAATCTTTGATGGGCACGGCGGCGAGAC TGCAGCGGAATATGTAAAATCTCGACTCCCAGAGGCTCTTAAGCAGCACCTTCAGGATTATGAGAAGGAC AAAGAAAACAGTGTTCTGACCTACCAGACCATCCTCGAGCAGCAGATCTTGTCAATTGACCGGGAAATGC TGGAAAAGTTGACAGTCTCCTATGATGAAGCAGGCACAACGTGTTTGATCGCTCTACTCTCAGATAAAGA CCTCACCGTGGCCAACGTTGGTGACTCTCGGGGAGTCTTGTGTGACAAAGATGGCAATGCCATCCCCTTG TCTCACGATCACAAGCCTTACCAGCTGAAGGAAAGGAAGAGGATAAAGAGAGCTGGTGGGTTCATCAGCT TTAATGGCTCCTGGAGGGTCCAGGGAATCCTAGCCATGTCTCGATCCCTGGGAGACTATCCACTGAAAAA TCTCAACGTGGTCATCCCAGACCCAGACATCTTGACCTTTGACCTGGACAAGCTGCAGCCGGAGTTCATG ATCTTGGCCTCAGATGGCCTGTGGGATGCTTTCAGCAATGAAGAAGCGGTTCGATTCATCAAGGAGCGCT TGGATGAGCCCCACTTTGGGGCCAAAAGCATCGTCCTGCAGTCCTTTTACAGAGGCTGCCCTGACAACAT CACTGTCATGGTGGTGAAGTTCAGGAATAGTAGCAAAACAGAAGAGCACTGAACCCTGCCAGATCTCAGC TGCCCCAAACTAGAGGACTCTCAACATACTGTTCTCTTCATGTAGTAAAAGGTGTGGGTATAATTAGGAT CATGCGTCCCAACACAGAACCCCCTTCCCTGATGGCCTTGAATCCCTTTGGAGTACTGAGCAGAGGGTTC CCTTGCTGACACCGCAGAGGCTGCTAAGTTTGTGTCCCCCCAGCCCTTCTATCAGTGTTTGAAACACATA CATAGGTAGCCACAGATCCCACATATGAGGCAAAGGAAAGGCAGGCCATATGTTTTCCTTCTTAATAATG TACTTTTCATGATCTCTTCTGTGGTCCTTCAGGGACCTGACTTGGCGTCCTCTCTGAACCTGGGCAGGGC AAGCTGGTGATGCCAACACTTCACCATGACAATGAGGTGGCCATGCCCTCTGCCTTGAAGCAGTTTCCTC AGAAATGCTCTCCCCTTCTGAGTCTTGGTCTTCAGTACTCCAGCATCCTGGAAGGAGACATGCAACACTT TGTAATTTGTTGCCTGCTCTTAGAGACTTGAGAGTTGGGAAGCCAGTGCAGCTTCCTGCACGCCAGACCT CCCCGCTTCCGCTCGCTTCCTTCATTAGTTCAGTAGAGTCGGGATGCAGTTAGCCAGCCCTTACGCCTCT CATGGAGTGCAGCCTCGCCATTTGTTCACACCTTACGATGCATCGAGGGCTGCCGCTGACCTCACCGTGA GACGTGAGTTTTTGTCTCCCCTCACCAAGACTCCCCAGAGAGGGGAGGAAGCAGGACGGAAAGCACATCG CAGTACAGTATGCCCTTCCATCTTCAAGGAGGAATTAGGCTGGGATGGTTCTCCATGACCTTTTGAAAAT TGCTGTCTGCACTGGAGAGGAAGGCAGGAGTCCTGGGGTCACCAGTCACACTCACCAACTGCTGGTTTCT GCACAGCCCTTCCCAAGCCCACCCTACCTAGTAGCTTTCATGCCTTTCAAACAGAGGTGGGAGAAACAGC AACCTAGAATCACCCACGGGTGCTGTTCCCAGCTACCACCACAAGTTGGTATCGGGGCGCAAACTACTGG GACGTGCTTCCTGGCAAGAGAAAGACCTTTCATGGTCAATTGCAAATCTCTGTCCATACCCAGGCAAATA GGAACTGTCTGCTGTGACCTGGAGGGAAAGAGCAGACTGGCTCCTGGGGACCTGTGTGCACTGGTTGGGA GTTCAAGCAGGAACCCTCAGAGATAAGCAGCAGAGTTATTTGGGGCCTAGATTACTGCTCTTCCCTTACT AACACCAAAGTTGTGGGGGTTTTTTTCCCAGTCTCTGTACTTTGTAAGTTAATTTATAGGACATATTAGA TTGTCCTGTTTGCTGATTGAACCAGCCCCAAAGAGCCACGATTTTCATTGCAGACCTACACCAGAGCCGG CCTTGTTTTGTTCTCTCAGAGAGGCTGTGTGTCGGCATGACTTCTCAAGCCCTGCTCATTAGCTCTCCCT ACCCCGGCCCTCTCCTTCAGCTGCAGAGCTTGTAGGAAGCCGAGTCTGGAGAATGAAAGACTTGGACTCT GAGGACTGCAAACTGTCTGGATTCCTCTGGGAACTTGCTTTCCTTTGGCTGCATCAAGAATTAATAATGG CTTCCCAGGAAATCTGCTTACTTTTGTATCTTGTGTTTTCAACATTGGAATTAGGGTCTTTTCTGCTTCT CAGAATGTTTTAGTACAAAACACATTCTATCATGTTAGACAGAAGCATTGATTGTGGAGAGACGTGCACT GATGGGTGTGTATTATGGGTTAGGTGGGTGGGGAACAGAGAAGTCAGGTGTGGGTTTAACCAGTGATGGG ATGAGCTTGTTGGCTTAGGATGCTCTTACCAGAGAGTACCCAAAACCCATCTTCTAACTAAAGGTCCAGA CTCAACAGCCTATACAGTCAACTTCCCTTCTGTATTTGCTTAACACAAAAGTATATAACAAGAGGAAAAA AATAAATAAATTTGAGACACAAATGTTTGGGTGAAGAAAAAAAGAAAACTGCCAAGTTAGTTGGTTGGTA CTCCTTGAGCAAGCAGGGTTTCTTAACCAGCATTGCTGAACCCCCCTAGTTCCAGAGCCTTCTAACTGAG GTTTATAGATATAGATATTATAGATCGATCTTGTACATTTGATCTTGATTTATAGATCTTGTACATTTTG CTTTGCAGAAGTAACTTATTTAACCAAATAGGAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6837066 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6837066 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12332 same embryo
	EMAGE:12333 same embryo
	EMAGE:12335 same embryo
	EMAGE:12334 same embryo
	EurExpress:euxassay_008476 same experiment
	MGI:4827366 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source