EMAGE mouse gene expression spatial database

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EMAGE:12436

Prune2 prune homolog 2 (Drosophila) ( MGI:1925004)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011113. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011113_01	euxassay_011113_02	euxassay_011113_03	euxassay_011113_04

euxassay_011113_05	euxassay_011113_06	euxassay_011113_07	euxassay_011113_08	euxassay_011113_09

euxassay_011113_10	euxassay_011113_11	euxassay_011113_12	euxassay_011113_13	euxassay_011113_14

euxassay_011113_15	euxassay_011113_16	euxassay_011113_17	euxassay_011113_18	euxassay_011113_19

euxassay_011113_20	euxassay_011113_21	euxassay_011113_22	euxassay_011113_23	euxassay_011113_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12436_wholemount_strong.wlz

12436_wholemount_moderate.wlz

12436_wholemount_weak.wlz

12436_wholemount_possible.wlz

12436_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12436_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

strong

regional

strong expression: see section 05 06 21 22 23

upper leg muscle

strong

regional

strong expression: see section 01 02 03 04 05 06 07 21 23 24

diaphragm

strong

regional

strong expression: see section 01 02 03 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 moderate expression: see section 04

hand

strong

regional

strong expression: see section 01

foot

strong

regional

strong expression: see section 21

lower leg rest of mesenchyme

strong

regional

strong expression: see section 24

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 moderate expression: see section 04

adrenal medulla

strong

regional

strong expression: see section 09 10 11 19 20

thymus primordium

strong

regional

strong expression: see section 13 14 15 16 moderate expression: see section 17

hypothalamus mantle layer

strong

regional

strong expression: see section 08 09 11 12 13 14 15 16 17

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 19

olfactory cortex marginal layer

strong

regional

strong expression: see section 11 12 13 15 16 17 18

medulla oblongata floor plate

strong

regional

strong expression: see section 13 14

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 09 10 11 17 18

metencephalon floor plate

strong

regional

strong expression: see section 13

rest of cerebellum mantle layer

strong

spotted

strong expression: see section 06 07 08 09 18 19

rest of cerebellum marginal layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 17 18 19 20 21 22

pons mantle layer

strong

regional

strong expression: see section 07 08 19 20

midbrain mantle layer

strong

single cell

strong expression: see section 07 17 18 19

tegmentum

strong

single cell

strong expression: see section 11

facial vii ganglion

strong

regional

strong expression: see section 05 06 07 08 20 21 22 23

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 19 20 21

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23 24

vagus x ganglion

strong

regional

strong expression: see section 09 19

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 10 18

trigeminal v nerve

strong

regional

strong expression: see section 10 18

ventral grey horn

strong

regional

strong expression: see section 12 13 14 15 16 17 18

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 11 12 13 16 17 18 19 20

neural retina

strong

regional

strong expression: see section 01 02 03 04 24

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 11 12 13 17 18 19

vomeronasal organ

strong

regional

strong expression: see section 13 16

esophagus

moderate

regional

moderate expression: see section 14 15

tongue muscle

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18

stomach

strong

regional

strong expression: see section 06 07 08 09 10 moderate expression: see section 02 03 04 05

midgut

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21

tail paraxial mesenchyme

strong

regional

strong expression: see section 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9330
Entity Detected:	Prune2, prune homolog 2 (Drosophila) ( MGI:1925004)
Sequence:	sense strand is shown >T9330 AACTAAAGTCACCCGTCCTAGATAGTCATGGAGACAAGTCACAGGAGAAACTTTGGAACATTCAACCTAA GCAGCTGGATTCAGATGCCAATCAACTCAGCCAGCTTGTGATCCTGGACCAAATGAAGGACAAAGATTCT GGACAGCAAACAGCCATGTCTCCTGCTGCTGGTGATCTTCCTGCAGAAACTCTCACCCAAGGGCAGGGCC GGGAATCTATGCTGTCTGTCTGGGACCGTGCAGAACCAGCATTAACTCACAGAGATGAAAATGGATGTGT TAGCACTGGGGTTTCACCCACTGAGTGCCAACAAGAGAACCAGTGGGAACCAGAAAAACCCTATCTCAGC CACGTGACACATTCAAGCACTCCCACAGAAAATGCCCTCGAGAGCAATGCCCCCACACAACTGATGAGGA AGCTGGACTCTGATTGGAATAGCCCCAGCCCCAGCGAACCCCAACATAACTTTGTTCCAGATATTTTACA TGGCAACTTCGAAGAGGGTGGGCAGCTGGCCTCGGCGTCACCTGATTTGTGGATGGATGTCAAGCAACCC TTCAGTTTTAAAGTAGATAGCGAGAATCCTGATATATTGACTCACTGTGACCATGACAGCAACTCTCAGG CATCCAGCAGCCCTGACGTGTGCCACGATTCTGAAGGGGAGCAAAAGATGGAGAAGCATACTGCTGTTTA CCTGGGACTTGAAGTGGAACCCAGTGAGTTTTCTCTCACCGAGCCAAACATGAATGATGAACCAACTTGG GAACCTGAGCAGGAAAGTCTCCCACACAATTCAGAACTCCATTCCGAACACGCAATGCCCTTGCCTCCAA TCGACAGTCAGAATGATATAAATAACTCATCTAAGCCTGCCTCTTCAAGAAGTTCCCCAGAACCCTCTGA TATGCGCGGAGACAACAACACAAGTGTAACATCGATGGAAGAAGACACCAACCCAGAGGTAGAAGCAGTG GACAGCGTGACGATCCCAGGTCACTTCCCCAGAAGTGAAGACGCTGACACTTTTGAAGCTCACCAAGAGG TCAGTGTGGAAGTCGACGACTCTTGGGTGTCAAAAGACCTTTGTCCTGAAAGTCAGACAGGCACTAGAGC TCTGCTTGACTGTGAGCAACCGTTTGCTTCCGAGAGCCCTGCTGTACTTACGGACATCTTCCTAACCTCA GACACCTGCCTGGATGTAAGCGAAGCTGCCTTGGATCACAGTTTCAGTGATGCCTCCGGCCTCAACACTT CCACAGGCACTATAGACGACATGAGTAAGCTGACTCTATCGGAAGGCCATCCCGAAACACCAGTTGACGG GGATGCAGGGAAGCAAGATGTCTGTTCATCTGAAGCTTCCTGGGGTGATTTTGAATATGATGCAATGGGT CAGAATATTGACGAAGAGCTGATGAGAGAGCCTGAACACTTCCTCTATGGTGGTGATCTTCCGTCAGAGG AAAGCGCGCTGAAGCAATCACTGACACCATACACACCCCCCTTTGATTTGTCCTATCTCACGGAACCTAC TGGATGCACTGAAACTGCACAGGGAGCTGAGTCTCCAGGTGATGAATCTCTGGGAAGTGACGCGGCAGAG ATGTTGCTTTCTGCTCTTCCAGATCACAGGGAGGAAGACAAGGCTGAGACCAACATCAGGAAACCTAGAT ACCAGATGACTGTGTTGCATATTCATGAAGACCCTGAGGCTCTTTCATCACCTGTGGGAGGAACAGGTTC CAATAATGAGTCATCTCCATCAAACATTGACTGGGAAATAGAAACAGATAATTCAGATTCACCAGCAGGT GGCGACATGAAACCACCAAACGGCAAGGAGATACTGGAGTTAGAAGAAGATGAGAAAGTAATTCCCACGA AAGGGCCAAAGCAAACAGAATTAGAATACAAAGAAGAAAAACAGCCAGAGCAAAGTGAAGACCATCAGGT ACTAGCTGTGGACTACATCCTTGTAAGCCATGAAAAAGATTCACCATTGAAACCAGAGGCCAGGGAAGCA AGAGAAAACATACCCGAATTGGAACAATTATCCATAGGTTCCAGAGAAACAGGGCTACCAGAGACTCAGT TATCAGGCACCCCAGACACATGTCAGTCTGAGTTTTTAAATGATGTCAAAGTTCATTCTGCAGAGAGAAT GTCTTCGTCTTCCAACCACGAGAGTGCATCTCTTGAAAACCCTGCACAAGACCAGAGTTGGATGGTCTTG AGCCATAGTGAGGTTGGTGACCCGCCTACAGAAACCAGGGACTCGGGGCCTGAGTCTCCTGGTAGAACTC CGGAGCCGTTCTTGAGTCTCAGTTTGGACAAGGGCCCCAAGTCCCAGGTTCTGGAAAGAAATAAGCCTCT AAATTCTTTAGCACTAGAGGAAGTTGCTGGTTTGAGCAGCCAATCAAGGAACATCGAGAGACAAGGTCAA GCTGGTCTAGATGCAGTTCCAACCCAGGCGGCCACCCACGACAATGAATGGGAAATGCTTTCGCCACAGC TCTCCCGGAAAAACAGGAACCCTCCACAGGAAATGGAAGAAGAGACACAGTGCCCTGAGCCTGGACCCAG AAAGCCAAGACTGAAAGGACCCCCGTCAGAGGATGAAGGAATGGATATCCACTTCGAGGAGGGAGTGCTG AGTCCCAGTGCCGCAGACATGAGGCCCGAACCTCCCAATTCTCTGGATCTCAATGGCAGTCACCCTAGAA GAATCAAGCTCACAGCCCCAAATATCAACCTCTCTCTGGACCAAAGTGAAGGATCTATTCTCTCTGATGA TAACCTAGACAGTCCGGATGAGATTGACATCAATGTGGATGAACTCGATACCCCTGATGAAGCAGACTCT TTTGAATATACTAACCATGAAGATCCCACGGCCAACAAAAGCTCTGGTCAAGAGTCAGAGTCTATTCCAG AATATACGGCTGAAGAGGAGCGAGAAGACAACCGGCTGTGGAGGACGGTGGTCATCGGGGAGCAGGAGCA ACGGATTGACATGAAGGTCATTGAACCCTACAGGAGAGTCATTTCTCACGGAGGATACTATGGGGACGGT CTGAATGCCATCATTGTGTTTGCTGCCTGCTTTCTGCCAGACAGCAGCCGGGCGGATTACCACTATGTCA TGGAAAACCTTTTCCTATATGTAATAAGTACCTTAGAGCTGATGGTTGCTGAAGACTACATGATCGTGTA CTTAAATGGTGCAACCCCAAGGCGGAAGATGCCAGGACTAGGTTGGATGAAGAAATGCTACCAGATGATT GACAGACGGTTGCGCAAGAATTTGAAGTCATTCATCATTGTTCACCCATCTTGGTTCATCAGGACAATCT TAGCAGTGACTCGGCCTTTTATAAGCTCTAAATTCAGCAGTAAAATTAAATATGTGACTAGCTTATCTGA GCTCAGTGGCCTGATCCCCATGGACTGCATCCACATTCCAGAGAGCATCATCAAGTATGATGAAGAGAAA TCTTTTAAGAGAAGTGTGAGACTGGATGAAGAACTGAGGGAGGCATCCGAAGCAGCTAAAACTAGCTGCC TTTACAATGATCCAGAAATGTCTTCTATGGAAAAGGACATTGACATGAAGCTGAAGGAGAAGCCGTAGCT GGCCTGCTGGAGGAGGACTCCCTTTCTGCTTGGTGATTTTGCTGGGACGTACCTACCTGGAAGCAACAGG GATGGTGACATCTCCACTTTGCACCACCTGGTGCCATTGGAAACTTTCAAGGAAAAGAGAATTTGTTTAC TGTTAACATATCCTATGAAATGTTGTGTCTTTTCCTATTTTGCCAATTACGTGCCTCAATGATTTTAGTT GAACCTTAGCAACTAAACCTTCCGTTTAGTTTTATGGACCTTCCATTTTTACTATTATGCTAATCTTCGG TGCAGTGGGACTTGGTTCCTTAGACTGGTTTTTACCAAAAGCATAGCCTAGTTCTGCTGTTAGACAGATG TGGATATGTTTCTGTAGACTGACTGAATCCCTACAGAGCTTCCTTCTAGATTCAGGATGCACAGGGCTTG CCTTGTCCCAGGAACATGGCTTTCTGGAACTCTATGAAATGCTGAACTTGAATAGGACAGACAGACAGAT ATAAAATCCTCATCAGCTAAGGCATCTGCCCCCTTCCTGTCCACATCTTTCAGTTTTTCAAAAGGACCCG GGCTTTCTAACCCATGCAAGCTTTACTTTATATTTCTGCATGTAAATTGCTCTTTTACAACATCATCTTC TAGTGAGCTTTGAAGAACTGCATCTTGTGAAACTTAAGACGAGCTATGCTTCAGGCCACAGAACAGGCTC CAACCTTTACACTTCGTCCAACTCCAAGGTGGCCCACTGAGAAATCACTGGGTAACATCGAATCGTCCAA AATTAACACTCAGATGCATTGCAAAAGCAATCTGCTTGAGGAAGTTTAAAATTAAATACAAGTATATACT AGCCTTTAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6848636 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6848636 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12437 same embryo
	EMAGE:12434 same embryo
	EMAGE:12435 same embryo
	EMAGE:12433 same embryo
	EMAGE:12438 same embryo
	EurExpress:euxassay_011113 same experiment
	MGI:4827461 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source