EMAGE mouse gene expression spatial database

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EMAGE:12438

Kcnd2 potassium voltage-gated channel, Shal-related family, member 2 ( MGI:102663)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011123. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011123_01	euxassay_011123_02	euxassay_011123_03	euxassay_011123_04

euxassay_011123_05	euxassay_011123_06	euxassay_011123_07	euxassay_011123_08	euxassay_011123_09

euxassay_011123_10	euxassay_011123_11	euxassay_011123_12	euxassay_011123_13	euxassay_011123_14

euxassay_011123_15	euxassay_011123_16	euxassay_011123_17	euxassay_011123_18	euxassay_011123_19

euxassay_011123_20	euxassay_011123_21	euxassay_011123_22	euxassay_011123_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12438_wholemount_strong.wlz

12438_wholemount_moderate.wlz

12438_wholemount_weak.wlz

12438_wholemount_possible.wlz

12438_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12438_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hand

strong

regional

strong expression: see section 01 02 03 moderate expression: see section 04 21 22 23

hindlimb digit 1

strong

regional

strong expression: see section 01 02 20 21 22

hindlimb digit 2

strong

regional

strong expression: see section 01 02 03 18 19 20 21 22

hindlimb digit 3

strong

regional

strong expression: see section 01 02 03 04 05 06 18 19 20 21 22

hindlimb digit 4

strong

regional

strong expression: see section 01 02 03 04 05 06 18 19 20 21 22

hindlimb digit 5

strong

regional

strong expression: see section 03 04 05 18 19 20 21

vibrissa

strong

regional

strong expression: see section 05 06 07 20 21 22 weak expression: see section 23

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

medulla oblongata alar plate mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

rest of cerebellum mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

pons mantle layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

midbrain mantle layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

spinal cord mantle layer

strong

regional

strong expression: see section 09 10 11 12 13 15 16 17

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 10 17

cervical ganglion

moderate

regional

moderate expression: see section 08 09 17 weak expression: see section 18

thoracic ganglion

moderate

regional

moderate expression: see section 12 weak expression: see section 13

extrinsic ocular muscle

moderate

regional

moderate expression: see section 05 06 20 21 weak expression: see section 02 03 04 22 23

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 14 15 16 17 18 19

vomeronasal organ

moderate

regional

moderate expression: see section 11 14

metanephros

moderate

regional

moderate expression: see section 07 08 09 10 16 17 18 19 20 21

male reproductive system

weak

regional

weak expression: see section 11 15

pelvic girdle skeleton

weak

regional

weak expression: see section 08 09 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9411
Entity Detected:	Kcnd2, potassium voltage-gated channel, Shal-related family, member 2 ( MGI:102663)
Sequence:	sense strand is shown >T9411 TGTGAGGGAAACCTGACCTTCTTGAGTACTGTGACTTGACCTGCGCCACCTTGGAATAATCGTGTTCCTG TGAGCAGTGTCCCCCCAGTGTTTTGCCTGAGGTGAAGAAGTGGGGGGCACTTTCTTACTTTCATTGTGTT ATTTTGGGTGACCCCCCTGCTCACTCTCGTGACCTCCACTTCCAGACAACATGGCAGCCGGTGTTGCAGC ATGGCTGCCCTTTGCCAGGGCAGCCGCCATTGGGTGGATGCCTGTTGCTTCGGGGCCTATGCCTGCGCCC CCAAGACAGGAGAGAAAAAGGACTCAGGACGCTCTGATAGTGCTGAACGTGAGTGGCACCCGTTTCCAGA CATGGCAAGACACCCTGGAACGATACCCAGACACTCTGCTGGGTAGTTCTGAGAGAGACTTTTTCTACCA CCCAGAGACCCAACAATACTTCTTTGACCGTGACCCGGACATCTTCCGCCACATCCTCAACTTCTACCGC ACGGGGAAGCTTCACTATCCCCGCCATGAGTGCATCTCGGCTTATGATGAAGAACTGGCCTTCTTTGGCC TCATCCCAGAAATTATTGGCGACTGCTGTTATGAGGAGTACAAGGACCGCAGGCGGGAGAATGCTGAGCG CCTCCAGGATGATGCAGACACTGACAATACAGGAGAGAGTGCGCTGCCCACCATGACTGCTAGGCAGAGG GTCTGGCGGGCCTTTGAGAATCCCCACACCAGCACCATGGCCCTGGTGTTCTACTATGTGACTGGGTTCT TCATTGCCGTCTCAGTCATCGCGAATGTGGTGGAAACAGTTCCATGTGGGTCTAGCCCAGGCCACATAAA AGAACTGCCTTGTGGGGAGAGGTATGCGGTGGCCTTCTTCTGCTTGGATACCGCCTGTGTCATGATCTTC ACAGTTGAGTACTTGCTTCGCCTGGCCGCAGCACCTAGTCGTTACCGTTTCGTGCGCAGTGTCATGAGTA TCATCGACGTGGTGGCCATCCTACCCTATTACATTGGGCTGGTGATGACAGACAATGAGGATGTCAGTGG AGCCTTTGTCACACTCCGAGTCTTTCGAGTCTTCAGGATCTTTAAGTTTTCCCGCCACTCTCAAGGGCTG CGTATACTGGGGTACACCCTGAAGAGCTGTGCATCAGAACTGGGCTTCTTGCTCTTTTCTCTCACAATGG CTATCATCATTTTCGCTACCGTTATGTTCTACGCAGAGAAGGGCTCTTCAGCAAGCAAGTTCACCAGCAT CCCGGCAGCCTTCTGGTACACCATCGTCACCATGACAACACTGGGGTATGGCGACATGGTACCAAAAACC ATAGCAGGGAAGATTTTTGGGTCCATCTGCTCACTGAGCGGAGTCTTGGTCATTGCGCTACCCGTGCCTG TGATCGTGTCGAACTTCAGTCGGATCTACCACCAAAACCAACGAGCAGACAAACGAAGGGCACAGAAGAA AGCCAGGCTGGCCAGGATCCGAGCAGCCAAAAGCGGGAGTGCAAATGCCTACATGCAGAGCAAGCGGAAT GGGCTACTGAGCAACCAGCTGCAGTCCTCGGAGGATGAACCGGCCTTCATAAGCAAATCTGGATCCAGCT TTGAGACACAGCACCACCACCTGCTTCACTGCCTGGAGAAAACCACGAACCATGAGTTTGTCGATGAACA AGTCTTTGAAGAAAGCTGCATGGAAGTTGCCACCGTCAATCGCCCATCAAGTCACAGTCCCTCTCTCTCC TCACAACAAGGAGTCACCAGCACTTGCTGCTCACGGAGACACAAAAAGACTTTCCGCATTCCTAATGCCA ATGTGTCGGGAAGCCATAGAGGCAGTGTGCAAGAACTCAGTACAATTCAGATCAGATGTGTGGAGAGAAC TCCACTATCCAACAGCCGATCCAGCTTAAATGCCAAAATGGAAGAGTGTGTTAAACTAAACTGTGAACAA CCTTACGTGACCACAGCAATAATAAGCATCCCAACACCTCCAGTAACCACCCCAGAAGGCGACGACAGGC CCGAGTCTCCTGAGTATTCGGGAGGAAATATCGTCAGGGTGTCTGCCTTGTAAGACAATTGCCGCAGGTC TGAGAGAATTTGAGCCCTGACTATGGAAAGAATCTAAAGGAGAAGAAAGAAGAAATAATAACCCCTTTTT GCATATTTAGACAGCAAAGCAAGAAGGTTGGCGATGAAACAAAAAAATCAAGATTTCAGAGTTGAAGATG TGAATCAAACCACCAAAAGGCATTTCTAGACAGCGTTCAACCTGTCCACAGAGGGTGACTCTGTGGCCCT TTGACTTTGTGAATGAGCACAATGAAATGCCATTTATTGATGCCTCTTATGATCAGAACTCTTTTTTAAT AAAACAAATAAAACAAATCTTTGGACATAATGTTCCAGTTGAATGCAAAACAAAAAAAATATGGAAAACA TTTTGATAAAAATTTTTCCTGTTAAAAACCATGAACATTGGCTCTGATGAAGATTGCTATTGATGAAAAA TTCATACGATCCATTTGTGTGGACTGAAGGAAACCATCCCAATGCATGCTAGAATTCTTTGGAGCAGTGA TCTCAGTTTCCTTATGTTGTCTTCAGAATAGGCATGACAAACTATACCTGTAGAAAGGGGAAATTTCTGT GCACTTACAATGAGCTGATTGTCCATGTTCTATGGTGGGCTGTGCAAATAAACTCCTTTTAGCACCACAG TGTTTCTCATGGGGATGCACACTAGTATATCTAAAGTGTTCGGATAGTTCAGTATTAATTATTGTTTAAC CTTGCACCTAGTCATTGTTATAACTGCAATAATTCATTCTATATCTGTTGTATCGGGAACCAGGATTTTT TTTTTGGACTTTCCATATATTCATAGATACAGTGAGAGCCACATTCATAGAAAAACTCCTGGTGTGGCCG GGTTTTAGCTGACTTTTTAATCCAAAACTACTGTGTCATAAATGTATTTTGGTAGTATTCTTTGGTCCAC TGTATTCCTGTGACCCAGTGCATTATCTGTCCCTGGATCTCTTTAGTGTCTTTCCACTGAGCTTACCCAC TAATGACAGGACTAGTGTCTACTGTAGTAGTAAAACAAACGTTCCCTGCCTTTGTATTTCTCAACCAGTT ATTATAAGAAGACTGTCACCAATCCCCTTGATAACAACTAGAGTAAAGGAGTTGACATGAAATATCTCCA GTGTTTCACTATTTGTAGAAAACCCAGACCCTGCAAACACCCATATGTGTTTTATGAGAGGCGTTAAATG GTACTTTTAAAAAAATCATAGGATGGATGATTTCAGAATCTAACTCAATGTATTAATTATTTTGCTTCAG ATCTTCAATACATTTTGAGGACAACTGGAATGCAGAGGAAAACTGAAGTGCTTTGTATAGCAATATTTGA TGAGAGCATGCTTTAAATGCCATCCAAGAAAGCAACAATGTATCTCAAAAGATTCTAGTGTATCACAAGG CACAAAGTTCTCTGCAAAATCAGGAAGAGGGAGTATGGTGATGTGAAGTCGACCACTGTGTACATTTAAG TGAAAAGTCTTGCTACCTTTTCACCCTTGAATATCCGCAGATGTGTCTGCACGTGACAATGTAAAAATAA AAAAAAAGTTTTATGTCAAATGAAAAGTTTTGTTCATTCCAAGCCACCACTGTAAGAACGAAAGCTTAGT TTCTATACATGAAAAAGAATTAATAATTAGTCCTCTGACATAGAGATTTTGAAATGCTTTTCACAATTTA TCATAAGAAAATGAATTAATCCAAACATTTTCAGGTAAAGCCAGAAATGGTCACTTCCCATTTATAGAAT AGCTTCTAGGACAGTGCTAGGCACACAGTAGCTCTTCATCAAAGCTGAGTGACAGGGAAAAGAACTCAGC TGACCCTCGGGAAGACCTGGCTTTACTCCTAGTGTGTCTTTGAAACAGTTACTAATATTCCTGAGACGTG AATATTAACAATTCTATTAACACCTGCCTGTTGTCACTTTATGCTTCTAGAGCAAATGTATAGTGAAACT TCTTTGATGGCACTTATTAAAAACTCTTTTAAAGTAAGTACAATCAGGAACATTTACCATCTTTTGATTC CCAATAAGAAATTCTATTTTCATGTTCCTAATAGGACATAAAACAACATAGACTTGGCTTATTTGGGTTG ATACTTATTATTCCTGCTTTGGTTTGTCGTTTATCACAAATTATTAATTTCTTCAGATTTTCAGAAGTGA AATTTTAAAAATAATCTTTTGGATGTTTTAGGTGATTTAAAATATACTGTGTTGGTGGTGAATGACTTTT GATGACGGTGTTAAGTGCAACTGTACTGTAAGTGAAATGTAATTATTTCTGTGTATCATATGGAGTAACT AAGGTCATTGTTTTTGACAATTTTGTTTGAAATTCATATATCTTATTTCAAAGGATAGCATAATATCTGC ATTATGCTGGAAAAAAATAGACCTTTGGAGAATACTTAAATAAAACATGTGCATGCTTGAACAGGAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30356567 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30356567 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12437 same embryo
	EMAGE:12434 same embryo
	EMAGE:12436 same embryo
	EMAGE:12435 same embryo
	EMAGE:12433 same embryo
	EurExpress:euxassay_011123 same experiment
	MGI:4825704 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source