EMAGE mouse gene expression spatial database

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EMAGE:12446

Opcml opioid binding protein/cell adhesion molecule-like ( MGI:97397)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011103. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011103_01	euxassay_011103_02	euxassay_011103_03	euxassay_011103_04

euxassay_011103_05	euxassay_011103_06	euxassay_011103_07	euxassay_011103_08	euxassay_011103_09

euxassay_011103_10	euxassay_011103_11	euxassay_011103_12	euxassay_011103_13	euxassay_011103_14

euxassay_011103_15	euxassay_011103_16	euxassay_011103_17	euxassay_011103_18	euxassay_011103_19

euxassay_011103_20	euxassay_011103_21	euxassay_011103_22	euxassay_011103_23	euxassay_011103_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12446_wholemount_strong.wlz

12446_wholemount_moderate.wlz

12446_wholemount_weak.wlz

12446_wholemount_possible.wlz

12446_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12446_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

epithalamus

strong

regional

strong expression: see section 13 14 15 16 17 18

diencephalon lateral wall mantle layer

weak

regional

weak expression: see section 10 11 12 13 17 18 19 20 21

telencephalon mantle layer

weak

regional

weak expression: see section 03 04 05 06 07 08 15 16 17 18 19 23 24

olfactory cortex mantle layer

weak

regional

weak expression: see section 18

olfactory cortex marginal layer

weak

regional

weak expression: see section 12 13 14 17 19 20

medulla oblongata alar plate mantle layer

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 21

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 21

rest of cerebellum mantle layer

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

pons mantle layer

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

midbrain mantle layer

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 17 18 19 20

facial vii ganglion

moderate

regional

moderate expression: see section 07 08 09 22 23 24

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 08 09 21 22

trigeminal v ganglion

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 20 21 22 23 24

trigeminal v nerve

moderate

regional

moderate expression: see section 11 12 19 20

spinal cord mantle layer

weak

regional

weak expression: see section 12 13 14 15 16 17 18 19

dorsal root ganglion

moderate

regional

moderate expression: see section 10 11 12 13 14 15 19 20 21 22

tongue

moderate

spotted

moderate expression: see section 14 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37329
Entity Detected:	Opcml, opioid binding protein/cell adhesion molecule-like ( MGI:97397)
Sequence:	sense strand is shown >T37329 TTCTAAAACTTCTCCCCTTCCCAAACCAATGCTAATTTGTTCTTCCACCAAAGTATGCCAATGAAAGTGC TAGTGTTCTGCCTCTGGCAAAGCCTGTTTTTTGAATAGTTTAATGTCAAGTGCCTGATACAGTCATCTGC AAGTTTAATCAAGAGTGTTTGGATTTTCTTTTTTTGTTCTCATTGGTTAGGTTGGAGACATAGTAGATTA GTTGTCAAAACATATACAGCTCTGACACAGAGAGCTAGGTATGTGGCTCTTCTGCTGTGGGCGAAGCTGT GTTCAACAGATGGAAATGGACATCTGTATGTCACCAAGATGGCTCATGCCTGTCCTTACACTGCTTTGGG CTGTTGTTCACAGGTTGGGAAGTTAGTTTTCAAAATATGGTCATAGGTTTGGTTTGGAATTCTAGGACCT TCATAACTGAGGCTGCATTTTAATGATCTCTTTCTATATCCATCTGGTCACATTGTCCTCAGCAAGAAGG AATAGCAAACCTGCCTACAATAGGAAAAATATCAAAAGAGCAGAGCCCCACCTTCCCCAAGTGGACACTG GATCCCAGAGAGTTTATCACAGGCACTGGATAAAGAAAAGTTGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 97179. Forward Primer - name:097179_F_cDNA_3732419F12, sequence:TTCTAAAACTTCTCCCCTTCCC; Reverse Primer - name:097179_N_SP6_cDNA_3732419F12, sequence:CCAACTTTTCTTTATCCAGTGCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12449 same embryo
	EMAGE:12448 same embryo
	EMAGE:12445 same embryo
	EMAGE:12447 same embryo
	EMAGE:12444 same embryo
	EurExpress:euxassay_011103 same experiment
	MGI:4826976 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source