EMAGE mouse gene expression spatial database

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EMAGE:12448

Cenpj centromere protein J ( MGI:2684927)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011118. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011118_01	euxassay_011118_02	euxassay_011118_03	euxassay_011118_04

euxassay_011118_05	euxassay_011118_06	euxassay_011118_07	euxassay_011118_08	euxassay_011118_09

euxassay_011118_10	euxassay_011118_11	euxassay_011118_12	euxassay_011118_13	euxassay_011118_14

euxassay_011118_15	euxassay_011118_16	euxassay_011118_17	euxassay_011118_18	euxassay_011118_19

euxassay_011118_20	euxassay_011118_21	euxassay_011118_22	euxassay_011118_23	euxassay_011118_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12448_wholemount_strong.wlz

12448_wholemount_moderate.wlz

12448_wholemount_weak.wlz

12448_wholemount_possible.wlz

12448_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12448_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 13 14 15 16 17 18

submandibular gland primordium

weak

regional

weak expression: see section 08 09 10 11 12 19 20 21 22 23

thyroid gland

weak

regional

weak expression: see section 13 18

pituitary gland

weak

regional

weak expression: see section 12 13 14 15 16 17 18

vibrissa

moderate

regional

moderate expression: see section 08 09 22 23 24

hypothalamus ventricular layer

weak

regional

weak expression: see section 13 14 15 16 17

diencephalon lateral wall ventricular layer

weak

regional

weak expression: see section 13 14 15 16

telencephalon ventricular layer

strong

regional

strong expression: see section 17 18 19 moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 weak expression: see section 02 03 04

rest of cerebellum marginal layer

strong

regional

strong expression: see section 17 18 moderate expression: see section 05 06 07 08 09 10 11 12 13 16 19 20 21 22 23 24

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 05 14 15 23 24

midbrain ventricular layer

strong

regional

strong expression: see section 17 18 moderate expression: see section 10 11 12 13 14 15 16 19

neural retina

moderate

regional

moderate expression: see section 01 02 03 weak expression: see section 04 05

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 11 12 13 14 17 18 19 20 21 22

vomeronasal organ

strong

regional

strong expression: see section 14 17

lower jaw incisor

moderate

regional

moderate expression: see section 13 14 17 18 19

lower jaw molar

moderate

regional

moderate expression: see section 08 09 21 22

upper jaw incisor

moderate

regional

moderate expression: see section 13 14 17 18 19

upper jaw molar

moderate

regional

moderate expression: see section 08 09 21 22

renal cortex

weak

regional

weak expression: see section 08 09 10 11 12 13 20 21 22 23 24

testis

weak

regional

weak expression: see section 07 08 23 24

lung

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9432
Entity Detected:	Cenpj, centromere protein J ( MGI:2684927)
Sequence:	sense strand is shown >T9432 CGATGGTTGTGGCGGCTCATTCGTAAATGCCGGGCCAGCTAAGTGGTGACTATCGCTGGTCGGAGCGGTC TGAACAGCTGTTTCTTCGAAGAACGGTGTTGGAAGTCAATAGGTGTTCTGGATTTTCCAGGGCTTAAATC GGGTCATTAGATTAAAATGTTCCTGATGCCAACCTCTTCAGAATTAAACAGTGGGCAGAATTTCCTAACC CAGTGGATGACCAGTCCTTCTCGGGCTGGGGTCATACTAAATCGAGGATTTCCTATTTTGGAAGCAGATG ATAAGCAAGCAGCTACAAATGTTTCTACGAGCTTCCCTGCTAAAGCCACCCATTTTTCAAATAGCTTCAG TATTAGTAGTGAAGAGGATTCATTTCATGAAGAACAGAAGTTGGAGGCTGGAGGACCTTATAAACCATGG TCAGAAAACCCTGAAGCTCCTCCGGTCTTTCCTTCAGTTAGAAAGGAGCCAATTGCATCACGTCAGGATG CCCCGGGATGCCAGGAGGATAACAACAATGATCTTACGCCTCATCTCGAGAGTGAATTCAAAGAAGTGGC TAATAAAAACCCACTTTTTAAAAAACTGGAACAGCTGAAAGAGATCCAGCAGAAGAAGCAGGAGCAGCTG AAGAGGCAGCAGCTGGAACAGCTGCAGAGGCTCATGGAGGAGCAAGAGAAGCTGCTCACCATGGTGTCTG CACAGCACGCGTTTCCAGGTACATTACTGCCTGATGATCAGAGCCAGAAGCATCGCTCTCCCGGAGATTT AACTCTGCCACCACACAGCTACTCAAATCCAACCCAAGAAAACTCCTGTGCTTCCAACGTTTTACCCGAT GAACAGAGTAATTTCTGTAGAGCCACTCAAGATTCCGTATTAACTTCAAAAAATGCTTCGGATTTGTTTT ACGAGTCACAGTATCAAGAAGCACATGTGAAAAGAAATGATTTAAAGGAGGAAAGTCCTGCCCACCCTTC AGGGGAAGGTGCTTTGCCACGTTGGGAAAAGAAGATGGGGAGGAGTCAGGAAGGGAAGGATGTGAACTTA CAAAAATGTGGAGATTCTTCAGAAGTGGTTAATATTGATGAAAGGCCCATCAAAGCTGCTGTTAGAGAGA AGCAGCAGACATTTGAGGACTACTTAGAAGAGCAGATCCAACTGGAAGAGCGGGAGCTTAGACAGAAGCA GCTGCAGGAAGCAGAAGGACCATTGCTAGCGAAAACGAAACCCAAGCAACCATTTTTAAAACGAGGAGAA GGTTTAGCTAGGTTTACTAATGCTAAATCTAAATTTCAAAAGGGGAAAGAAAGTAAGCTAGCAAGTACGC AGAGCCCATCAGAGGACCAGCCTGGGTCTAAAGTAGACAGACAGCATTTGCAGAGGAAAACTGCTCTCAT AAATAAAGACCTGTGTGCAGAAACCCCCACTGTTAAAAAGGACAGTAAAGCTAGACCCAAGGCTGGCTTT GCCTCACTCAGGCAGAAACCCAAAGTGACCAAGACCAATATGAGAGAAAGTCTCTCCCCACCAGGACTGA AAGTCCAGACGGGGAAGAAACGTGATGGACAGTTTAGACACCAGGTCAAAGGAGAACGAAATGCACATGC TAGTAATAAGGAAAATGTGCCTGCATGCATAAAGCCCTGGGATGCTGGTTGCAAGATGTGGAGTAAGACA CAGGGGAGAGAGAGACTTCCTCTTTCCACTGGACCAGTTGGCTGTGTGGTCTCTAGGAGCCCGATACGTG AGACAGACAGAGAAACAGAATCTTCCCTTGATTTTTCTCTTCAGAAAAAATTAGAGATTTGGGAACGAGA AAAAGAAAAGGAAAACTTGGAATTAGATGAATTTTTATTTTTGGAACGGGCTGCTGATGAAATATCATTT TCTAGTAATTCCTCATTTGTACTGAGAATCTTAGAAAGGGACCAGCAGATCTGCGACGGTCACAGGCTGT CTTCTACTCCTGTCAAAGCTGTACAGCAGCGTGAGGCACAGCAGGCAGATCCTAGGGGTCAGAGTAACTG CAGTGAGATTCCCCGTTACGGTGTCGCACATGAGAACGAGAGTGAATGCGAGGCCATGCTCTTGTCTTGG GGTTCAGGGTCACCTGATGGTTTGAGGGAACTGTCCTGTAAACGTAGTATGAAAGCCTTCCAAACCAGCA CTTCTGAGATCCAGAGCCAATGGGATGCAAGGGATGATGGCGTTGCCAACAGCGACAGTAGCACTGAGTC TGAGGAGCAGCATGACATTACCATCAAACCGTCCACCGAGGTTGGGGACAGGGTCTTCAGCAGCAGAGAG GATAGTCCACAAGTCTGTGATGCCAAAGGACCTATTAGGGACACCGGGGCTCAGGAAGATAAATGGAGAG ACGCAGACCTGGATCTGTCTGATAAAGAGTGTAGCAGTGACGAGTCTGTCATAGTTGAAAGCCTGAACAA TAAAGTATTGGAACCACTGAGACTGCCCTCCTCTCAAGCTGGGAGTAAAATTGACTTCGATGATGAAAGA TCGTGGACAGACCTTGAGGAGAATCCATACGAACATGGTGTTATCCACAGAGAGGAAGCCATATATGGTA CACCCCAGACACAGTGCCACAGTAAGAGTGAAGGGTGTGTTCTGGACAAAACAATAAAAAGGAAGATTGC ACCAGTCAAGAAGGGAGAAGACTTCAAGTGTGACAGGCGCATAAGCCCTCCTCCTCCATCTGACTTGATG GTAAAATTCTTCCCTTCTTTGAAACCAAAGCCAAAATTAGATTCACACTTGGAAAATGAATCCAAGTTAA ACCTGAGTCAAGATCAACCACCAGAATTTATGGTCTGTTTTATAGGTGACAGTGTTCGATCTCAGGTTTT AAGAGAGAAAGTTACTGAATTGGAATCAGAAATAGAAAAATTTAAAGCTGAGAACACATCTTTAGCTAAA CTTCGTATTGAACGAGAAAGTGCCTTGGAAAAACTGAGGAAAGAAATTGCTGACTTTGAGCAACAAAAAG CAAGAGAGCTGGCTCGAATAGAAGAGTATAGAAAGGAGGAGACAAGGAAACTACAAAAGGAGCGCAAAGT CTTTGAGAAGTACACAGCAGCTGCAAGAACATTCCCAGATAAAAAGGAGCGCGAAGAAATACAGGCCTTG AAACAGCAAATAGCAGATTTACAGGAAGACTTGAAAAGGAAGGAAACAAAGTGGTCAAGCACACAGAGTC GCCTCAGAAGCCAGATAGAGATGCTCGTCAAAGAGAACACAGACCTCAGGGAAGAGATAAAAGTAATGGA AAGGTTCCGACTGGATGCCTGGAAGAGAGCAGAAGCTATGGAGAACAGTCCCAAGGCCTGCCAGTACATG ATGGCCACAAAAAAGGATGAGTCCATGAACTCATCATTTCAATTTCAAAAGAGTCATGTTTCTTCAGGAG TCCAGGTAGAAAAATATAAGAAAAAGTATTTGCCAGCACAAGGAAATCTATCTAGAAGAATCAAGTCTGC GCCTCCTCGTGATTTAGGCAGCTCCGATAAAGGACAAGCTGCCTTGCCCAGAGAGCCTCTTCAACAAGTG AACTTCCCAGACCTTGAATATAAAAACAAAGAGGAAAAAGAGGAAGAAATTCAGGGAGAAATCAGTCATC CTGATGGAAAGGTAGAAAAAATTTATAAAAATGGGCGCCGGGTTGTTCTGTTTCCCAATGGAACTCGGAA AGAAGTGAGTGCGGATGGGAAGAGCGTCACCGTTACATTTTTCAATGGTGATGTGAAGCAGGTCATGCCA GATGAGAGAGTGGTCTACTACTATGCTGCTGCCCAGACCACACACACCACGTACCCAGAAGGACTTGAAG TTTTACATTTCTCAAGTGGACAAATAGAAAAGCATTTCCCAGATGGAAGAAAAGAAATCACATTTCCTGA CCAGACCATTAAGACTTTATTTGCTGATGGTCAAGAAGAGAGCATCTTTCCAGATGGCACAATTGTCCGA GTACAACGTGATGGCAACAAGATCATAGAATTTAATAATGGCCAAAGGGAGCTCCATACTGCCCAGTTCA AGAGACGAGAGTATCCCGATGGGACTGTTAAGACTGTCTATGCAAATGGTCATCAAGAGACAAAGTACAC ATCTGGCCGAGTTAGAGTGAAGGACAAAGACGGTAATGTGCTAATGGACACAGAAATGTGAAGATCTTTT GTGGGATTAATCATGAAGTAACACTAAGTGCTTTTTCTGTTCAGAGTATGTACATCTCTTATATGTTCAA TTGTTTAATTTATATAATGTGTGTGTCTAAATAAAAAGATTATGTTTGAATTTTAAAATTAAAGTTTTAC CATACTGCAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30549055 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30549055 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12446 same embryo
	EMAGE:12449 same embryo
	EMAGE:12445 same embryo
	EMAGE:12447 same embryo
	EMAGE:12444 same embryo
	EurExpress:euxassay_011118 same experiment
	MGI:4823808 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source